Western Blot analysis of rat eyeball lysate (20ug/lane) using 036387 (1:2000). Secondary IgG (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 120kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of formalin fixed, paraffin embedded human thyroid tissue using 036387 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of 036387 for immunohistochemistry.

Overlay histogram showing Hela cells stained with 036387 (green line). The cells were fixed with 2% paraformaldehyde (10mins). The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by 036387 (1:25) for 60mins at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Flow Cytometric analysis of Ramos cells using 036387 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.