Fig 2: Sensory nerve-derived Sema3A promotes osteogenesis and angiogenesis in SCS-mediated bone regeneration. (A) Micro-CT analysis of bone regeneration at 12 weeks. 3D reconstructed superficial and interior images of femoral condyle defects in different groups. Scale bar: 4 cm. (B) Quantitative analysis of bone volume/total volume (TV/BV), bone mineral density (BMD) and Trabecular Thickness (Tb.Th) (n = 6). (C) Representative images of immunofluorescence staining of Leptin Receptor and CGRP. (D) Representative images of immunofluorescence staining of CGRP and Emcn. (E) Representative images of immunofluorescence staining of CGRP and CD31. (F) Quantification of CGRP, LepR, Emcn and CD31 median fluorescence intensity (MFI) per field view. Nuclei were stained with DAPI (blue). Scale bar: 100 μm. For all charts, data represent means ± standard deviations (n = 6). Groups labeled with different lowercase letters are significantly different (p < 0.05).