Human IGF-II R/IGF2R Antibody AF2447 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman IGF-II R/IGF2R Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF2447
NCBI Gene AliasesMPR300, M6P/IGF2R, MPR 300, MPRI, MPR1, CI-M6PR, CD222, CIMPR, M6P-R
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,In vivo assay,Blockade of Receptor-ligand Interaction,Neutralization,Immunocytochemistry,CyTOF-ready
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Rat
HostGoat
Conjugate/TagUnconjugated
TargetIGF-II R/IGF2R
IsotypeIgG
NCBI Full Gene Nameinsulin like growth factor 2 receptor
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived recombinant human IGF-II R/IGF2R, Ser1510-Phe2108, Accession # P11717
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunohistochemistry in human tissues?

Yes, the IGF-II R/IGF2R antibody has been shown to work in immunohistochemistry applications, as demonstrated in studies where it was used to stain tumor tissues in xenograft experiments (, ).

What species does this antibody react with?

The IGF-II R/IGF2R antibody is reactive with mouse, human, and rat species, making it suitable for a variety of experimental models (, ).

Has this antibody been utilized in any specific disease models?

Yes, the antibody has been used in studies related to cancer, such as in the context of melanoma resistance to therapy, where it was involved in evaluating the expression of mannose-6-phosphate receptor in tumor cells ().

What is the recommended concentration for using this antibody in Western blotting?

In Western blotting applications, the IGF-II R/IGF2R antibody has been used at a concentration of 0.1 µg/ml, as noted in various studies (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Microglia-neuron crosstalk through Hex-GM2-MGL2 maintains brain homeostasisNatureOctober 1, 2025Frosch M, Shimizu T, Wogram E, Amann L, Gruber L, Groisman AI, Fliegauf M, Schwabenland M, Chhatbar C, Zechel S, et al.10 mM stock in DMSO), M6P (10 mM; prepared in neuron medium), IGF2R-blocking antibody (20 µg ml−1; AF2447, R&D Systems) or isotype control IgG (20 µg ml−1; AB-108-C, R&D Systems). Following preincubation, recombinant

(2) A role for CIM6P/IGF2 receptor in memory consolidation and enhancementeLifeMay 5, 2020Yu XW, Pandey K, Katzman AC, Alberini CM.rats were excluded due to incorrect cannula placement. Anti-CIM6P/IGF2R antibody (R and D Systems # AF2447, Minneapolis, MN, USA) or IgG control was dissolved in PBS and injected at the indicated times at 5

(3) Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophyEMBO molecular medicineJanuary 9, 2020Bella P, Farini A, Banfi S, Parolini D, Tonna N, Meregalli M, Belicchi M, Erratico S, D'Ursi P, Bianco F, et al.injected into the tail vein with 10 μg (low dose) or 100 μg (high dose) of Polyclonal Goat IgG Anti‐IGF2R (AF2447, R&D Systems, Minneapolis, MN) (Chen et al, 2011; Lee et al, 2015) and treated for 4 or 9 weeks. In

(4) Wilm's tumor 1 promotes memory flexibilityNature communicationsAugust 21, 2019Mariottini C, Munari L, Gunzel E, Seco JM, Tzavaras N, Hansen J, Stern SA, Gao V, Aleyasin H, Sharma A, et al.10 μM. The antibody against the IGF2 Receptor (IGF2-R Ab) was purchased from R&D solutions (catalog #AF2447) and used at a final concentration of 5 μg/ml. Transcriptomic profiling by mRNAseq For the mRNAseq

(5) Maternal transmission of an Igf2r domain 11: IGF2 binding mutant allele (Igf2r(I1565A)) results in partial lethality, overgrowth and intestinal adenoma progressionScientific reportsAugust 6, 2019Hughes J, Surakhy M, Can S, Ducker M, Davies N, Szele F, Bühnemann C, Carter E, Trikin R, Crump MP, et al.USA) was diluted 1 in 20000 in the blocking reagent. Antibodies used included anti-IGF2R (R&D systems, AF2447). Surface plasmon resonance Surface plasmon resonance was performed on a BIAcore® T200 for human IGF2

(6) Insulin Stimulates PI3K/AKT and Cell Adhesion to Promote the Survival of Individualized Human Embryonic Stem CellsStem CellsAugust 1, 2019Godoy-Parejo C, Deng C, Liu W, Chen G.Blocking Ab,Millipore,MAB1137Z IGF1R Blocking Ab,BD Pharmingen,555998 IGF2R Blocking Ab,R&D Systems,AF2447 TNNT2,DSHB,CT3 Integrin β1 Blocking Ab,DSHB,AIIB2 Integrin α6 Blocking Ab,DSHB,P5G10 GAPDH,Santa Cruz

(7) BRAF Targeting Sensitizes Resistant Melanoma to Cytotoxic T CellsClinical cancer research : an official journal of the American Association for Cancer ResearchMay 1, 2019Atay C, Kwak T, Lavilla-Alonso S, Donthireddy L, Richards A, Moberg V, Pilon-Thomas S, Schell M, Messina JL, Rebecca VW, et al.tissue were prepared and stained with polyclonal goat M6PR/IGF2R primary antibody (R&D Systems; Cat.no. AF2447; 5 μg/mL per samples) O/N at 4°C. Biotinylated 2nd antibody in 1:200 dilution was used for 30 min at

(8) The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's diseaseActa neuropathologica communicationsJanuary 31, 2019Gerber H, Mosser S, Boury-Jamot B, Stumpe M, Piersigilli A, Goepfert C, Dengjel J, Albrecht U, Magara F, Fraering PC.NY), PA5-51134 (for PTGFRN, 1:1000, Thermo Fisher, Waltham, MA), AF5320 (for M6PR-CD, R&D Systems), AF2447 (for M6PR-CI, R&D Systems). Same approach was used for the Western blot analysis of APMAP and associated

(9) IGF-II promotes neuroprotection and neuroplasticity recovery in a long-lasting model of oxidative damage induced by glucocorticoidsRedox biologyOctober 1, 2017Martín-Montañez E, Millon C, Boraldi F, Garcia-Guirado F, Pedraza C, Lara E, Santin LJ, Pavia J, Garcia-Fernandez M.

(10) mTORC1 regulates mannose-6-phosphate receptor transport and T-cell vulnerability to regulatory T cells by controlling kinesin KIF13ACell discoveryJanuary 1, 2017Ahmed KA, Xiang J.

(11) A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell lineInternational journal of oncologyOctober 1, 2014Benabbou N, Mirshahi P, Bordu C, Faussat AM, Tang R, Therwath A, Soria J, Marie JP, Mirshahi M.

(12) Maternal transmission of a humanised Igf2r allele results in an Igf2 dependent hypomorphic and non-viable growth phenotypePloS oneJanuary 1, 2013Hughes J, Frago S, Bühnemann C, Carter EJ, Hassan AB.milk. Antibodies used were anti-IGF2 (R&D systems, AF792) at 0.4 µg ml− 1, anti-IGF2R (R&D systems, AF2447) at 0.1 µg ml− 1, anti-Cathepsin L (R&D systems, AF1515) at 0.1 µg ml− 1. After extensive washing, the

(13) Elevation of IGF-2 receptor and the possible underlying implications in end-stage heart failure patients before and after heart transplantationJournal of cellular and molecular medicineMay 1, 2012Wei Y, Li J, Huang J, Zhang X, Zhao H, Cui C, Li Y, Hu S.previously [16]. Primary antibodies used included goat anti-human IGF-2R antibody (0.1 μg/ml, Cat. No. AF2447; R&D System, Inc., MN, USA), and mouse anti-human antibody for Glyceraldehyde 3-phosphate dehydrogenase

(14) IGF2 actions on trophoblast in human placenta are regulated by the insulin-like growth factor 2 receptor, which can function as both a signaling and clearance receptorBiology of reproductionMarch 1, 2011Harris LK, Crocker IP, Baker PN, Aplin JD, Westwood M.

(15) A critical role for IGF-II in memory consolidation and enhancementNatureJanuary 27, 2011Chen DY, Stern SA, Garcia-Osta A, Saunier-Rebori B, Pollonini G, Bambah-Mukku D, Blitzer RD, Alberini CM.

(16) Expression of growth factor receptors and targeting of EGFR in cholangiocarcinoma cell linesBMC cancerJune 18, 2010Xu L, Hausmann M, Dietmaier W, Kellermeier S, Pesch T, Stieber-Gunckel M, Lippert E, Klebl F, Rogler G.antibody, mouse monoclonal anti-human IGF1R antibody (#MAB391, R&D Systems), goat anti-human IGF2R (#AF2447, R&D Systems) antibody, mouse monoclonal anti-human VEGFR-1 antibody (IgG1, DM 3504, Acris Antibodies,

(17) CREG inhibits migration of human vascular smooth muscle cells by mediating IGF-II endocytosisExperimental cell researchNovember 15, 2009Han Y, Cui J, Tao J, Guo L, Guo P, Sun M, Kang J, Zhang X, Yan C, Li S.

(18) Urokinase induces survival or pro-apoptotic signals in human mesangial cells depending on the apoptotic stimulusThe Biochemical journalOctober 15, 2008Tkachuk N, Kiyan J, Tkachuk S, Kiyan R, Shushakova N, Haller H, Dumler I.

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IGF-II R/IGF2R Antibody

IGF-II R/IGF2R Antibody

IGF-II R/IGF2R Antibody

IGF-II R/IGF2R Antibody

IGF-II R/IGF2R Antibody

Human IGF-II R/IGF2R Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (18)

Reviews

Human IGF-II R/IGF2R Antibody from R&D Systems, a Bio-Techne Brand

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