ASCL2/Mash2 was detected in immersion fixed paraffin-embedded sections of human placenta using A3619-81B at 10ug/ml for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG HRP Antibody (HRP). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in extravillous trophoblast.