Western blot analysis is shown using Affinity Purified Human WHIP to detect Human WHIP present in a HEK293 whole cell lysate.~30?g of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.kD is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution at room temperature for 2h followed by detection using Goat-a-Rabbit IgG diluted 1:5000 for 45 min. Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results.
Western blot analysis is shown using Human WHIP with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1.~30?g of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0kD is detected. The blot was incubated with a 1:1000 dilution at room temperature for 2h followed by detection using Goat-a-Rabbit IgG diluted 1:5000 for 45 min. Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. Other systems will yield similar results.
Supplier Page from United States Biological for Rabbit Anti-WHIP Antibody