Western blot analysis of AK2 on different lysates with Rabbit anti-AK2 antibody at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: HL-60 cell lysate Lane 3: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 26 kDa Exposure time: 2 minutes; 12 % SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody AK2 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.