Western blot analysis of GABRB2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500289, 1/1,000) was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human Brian tissue lysate Lane 2: Rat cerebellum tissue lysate Lane 3: Rat Brian tissue lysate Predicted band size: 59 kDa Observed band size: 80/50/65 kDa (Glycosylation)