Fig 1: Gating strategy for panel B to identify naïve and activated CD4+ or CD8+ T cells(A) Lymphocytes are gated on the side (SSC) and forward (FSC) scatter plot, followed by a gate to identify singlets. Gated on singlets, CD45+ cells confirm the presence of leukocytes in the tissue (spleen is shown). Gated on CD45+ cells, live versus dead cells are distinguished using Zombie Aqua. Gated on live cells, CD4+ or CD8+ T cells are then gated on expression of CD62L, with naïve CD4+ or CD8+ T cells exhibiting a CD44loCD62Lhi profile and conventionally activated CD4+ or CD8+ T cells T cells exhibiting a CD44hiCD62Llo profile. Resting memory phenotype T cells have been also described as CD44hi. Representative plots of (B) CD11a+ splenic CD4+ and CD8+ T cells, and (C) PD-1+ CD8+ T cells in the skeletal muscle. Fluorescence Minus One (FMO) gating controls are shown.