Western blot analysis of PIWIL4 using PIWIL4 Polyclonal Antibody (Product # PA5-116559) at a 1:3,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of PIWIL4 in extracts of various cell lines. Samples were incubated with PIWIL4 Polyclonal antibody (Product # PA5-116559) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.