Fig 1: Differentially expressed genes (DEGs) in Per2 KO and WT mice. (A) DEGs between Per2 KO and WT mice before and after METH administration in GO functional analysis associated cellular components. Five genes (Chrm2, Ephb2, Htr1b, Oprm1, Stx1b; in red box) associated with receptors in the presynaptic membrane were downregulated only between drug-naive Per2 KO and WT mice, with no differences after METH treatment. (B–F) The selected five genes were validated using qRT-PCR in Per2 KO and WT mice. (G) Weight of Per2 KO and WT mice. (H) The percentage of brain/body. *p < 0.05, significantly different by t-test
Fig 2: Nonlinear fit analysis of the relationship between target protein expression levels and cognitive function in the Y-maze generated using the bell-shaped dose–response curve model. (A) PER2 Plateau1: 66.97, Plateau2: 52.52, Peak: 10.44, LogEC50_1: 2.41, LogEC50_2: 2.564, nH1:0.95, nH2: 3.91; (B) EPHB2 Plateau1: ~ − 294.5, Plateau2: 54.69, Peak: ~ − 308.9, LogEC50_1: 1.07, LogEC50_2: 2.75, nH1:4.40, nH2: 2.73; (C) OPRM1 Plateau1: 53.51, Plateau2: 72.48, Peak: 57.29, LogEC50_1: 4.68, LogEC50_2: 2.83, nH1:80.36, nH2: -4.15; (D) CHRM2 Plateau1: 63.11, Plateau2: 63.15, Peak: 28.39, LogEC50_1: ~ 1.03, LogEC50_2: 1.02, nH1: ~ 697.6, nH2: 12.63; (E) HTR1B Plateau1: ~130.8, Plateau2: ~-22.36, Peak: ~42.2, LogEC50_1: 0.47, LogEC50_2: ~1.28, nH1:-12.64, nH2: ~-0.09;. Relationship between (F) EPHB2 and PER2 and (G) OPRM1 and PER2. Correlations were presented as fold change relative to β-actin and cognitive abilities of WT mice over 24 h seen in western blot and Y-maze test
Supplier Page from MyBioSource.com for HTR1B Rabbit pAb