Indirect immunostaining of PFA fixed mouse spleen section with rabbit anti-CD8a (cat. no. HS-361 003, dilution 1 : 100; green) and rat anti-CD4 (cat. no. HS-360 017, dilution 1 : 100; red). Nuclei have been visualized by DAPI staining (blue). Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) has been applied before staining. Magnification 200x.​

Indirect immunostaining of PFA fixed mouse spleen section with rabbit anti-CD8a (cat. no. HS-361 003, dilution 1 : 100; green) and rat anti-CD4 (cat. no. HS-360 017, dilution 1 : 100; red). Nuclei have been visualized by DAPI staining (blue). Antigen retrieval (10mM citrate, pH 6.0, overnight at 60°C) has been applied before staining. Magnification 400x.​

Indirect immunostaining of PFA fixed paraffin embedded mouse spleen section with rabbit anti-CD8a (cat. no. HS-361 003). Immunoreactivity was revealed using ABC secondary detection system and diaminobenzidine as chromagen. Nuclei were counterstained with haematoxylin (blue). ​

Indirect immunostaining of formalin fixed paraffin embedded mouse spleen section with rabbit anti-CD8a (cat. no. HS-361 003; green) and rat anti-CD3e (cat. no. HS-413 117; red). Nuclei have been visualized by DAPI staining (blue).​