STING/TMEM173 Antibody AF6516 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSTING/TMEM173 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF6516
NCBI Gene AliasesMPYS, hSTING, hMITA, ERIS, STING, TMEM173, SAVI, STING-beta, NET23, MITA
IsotypeIgG
TargetSTING/TMEM173
ApplicationsCo-Immunoprecipitation,Immunoprecipitation,Western Blot,Simple Western,Immunocytochemistry
HostSheep
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse
NCBI Full Gene Namestimulator of interferon response cGAMP interactor 1
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant human STING/TMEM173, Ala215-Ser379, Accession # Q86WV6
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
PurityAntigen Affinity-purified
ConcentrationLYOPH

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human STING/TMEM173 in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study STING's role in viral infections?

Yes, the STING/TMEM173 antibody has been utilized in studies examining its role in viral infections. For instance, in the research by Bodda et al. (2020), the antibody was employed to investigate how the herpes simplex virus inhibits type I interferon expression by deubiquitinating STING, highlighting its importance in the immune response to viral DNA.

Is this antibody effective for immunocytochemistry applications?

The STING/TMEM173 antibody has been shown to work in immunocytochemistry, as demonstrated in studies such as Hansen et al. (2014), where it was used to visualize STING in human macrophages following stimulation with bacterial DNA, indicating its utility in cellular localization studies.

What species can this antibody detect, and is it validated for use in knockout models?

This antibody is reactive with both human and mouse STING, making it suitable for studies involving these species. Additionally, it has been knockout validated, ensuring its specificity and reliability in experiments involving genetically modified organisms.

How has this antibody been utilized in research related to autoimmune diseases?

The STING/TMEM173 antibody has been referenced in studies investigating its role in autoimmune conditions, such as systemic lupus erythematosus. For example, Prabakaran et al. (2021) utilized this antibody to explore STING's involvement in inflammatory responses, suggesting its relevance in understanding STING-related pathologies.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) TRIM23 mediates cGAS-induced autophagy in anti-HSV defenseNature communicationsMay 13, 2025Acharya D, Sayyad Z, Hoenigsperger H, Hirschenberger M, Zurenski M, Balakrishnan K, Zhu J, Gableske S, Kato J, Zhang SY, et al.custom made by GenScript), anti-p-S172-TBK1 (1:500, 558397; BD Biosciences), and anti-STING (1:500, AF6516; R&D Systems) diluted in PBS containing 1% (w/v) BSA at 4 °C on a rocking platform. Cells were washed

(2) Distinct pathogenic mutations in ARF1 allow dissection of its dual role in cGAS-STING signallingEMBO ReportsMarch 24, 2025Johannes Lang, Tim Bergner, Julia Zinngrebe, Alice Lepelley, Katharina Vill, Steffen Leiz, Meinhard Wlaschek, Matias Wagner, Karin Scharffetter-Kochanek, Pamela Fischer-Posovszky, et al.antibodies mouse anti-FLAG M2 (1:5000, Sigma-Aldrich, F1804), sheep anti-STING (1:1000, Bio-Techne, AF6516), rabbit anti-STING (1:1000, Proteintech, 19851-1-AP), rabbit anti-TFAM (1:1000, Proteintech, 22586-1-AP),

(3) STING-induced noncanonical autophagy regulates endolysosomal homeostasisProceedings of the National Academy of SciencesFebruary 25, 2025Tuozhi Huang, Chenglong Sun, Fenghe Du, Zhijian J. ChenRab10 (#8127) were purchased from Cell Signaling Technology. The antibody for STING immunofluorescence (AF6516) was purchased from R&D systems. Antibodies for ATP6V1A (ab199326), ATP6V1D (ab157458), FNIP1 (ab134969),

(4) The ubiquitin E3 ligase TRIM10 promotes STING aggregation and activation in the Golgi apparatusCell reportsApril 25, 2023Kong L, Sui C, Chen T, Zhang L, Zhao W, Zheng Y, Liu B, Cheng X, Gao C.

(5) STING nuclear partners contribute to innate immune signaling responsesiScienceSeptember 24, 2021Dixon CR, Malik P, de Las Heras JI, Saiz-Ros N, de Lima Alves F, Tingey M, Gaunt E, Richardson AC, Kelly DA, Goldberg MW, et al.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Rabbit polyclonal anti-Lamin A,ECS lab,3262 Sheep polyclonal anti-STING,R&D Systems,Cat# AF6516; RRID:AB_10718401 "Rabbit polyclonal anti-IRF3, FL-425",Santa Cruz Biotechnology,Cat# sc-9082; RRID:AB_2264929 Rabbit

(6) A STING antagonist modulating the interaction with STIM1 blocks ER-to-Golgi trafficking and inhibits lupus pathologyEBioMedicineApril 1, 2021Prabakaran T, Troldborg A, Kumpunya S, Alee I, Marinković E, Windross SJ, Nandakumar R, Narita R, Zhang BC, Carstensen M, et al.for immunoblotting anti‐STING (Cell Signaling, D2P2F/#13,647, 1:1000), sheep anti‐STING (R&D Systems, AF6516, 1:500), rabbit anti‐phosphoSTING (S366) (Cell Signaling Technology, #85,735, 1:1000), rabbit anti‐TBK1

(7) STEEP mediates STING ER exit and activation of signalingNature immunologyAugust 1, 2020Zhang BC, Nandakumar R, Reinert LS, Huang J, Laustsen A, Gao ZL, Sun CL, Jensen SB, Troldborg A, Assil S, et al.follows: rabbit anti-STING (Cell Signaling, D2P2F/#13647, 1:1,000), sheep anti-STING (R&D Systems, AF6516, 1:500), rabbit anti-pSTING (S366) (Cell Signaling Technology, #19781, 1:1,000), rabbit anti-TBK1 (Cell Signaling

(8) HSV1 VP1-2 deubiquitinates STING to block type I interferon expression and promote brain infectionThe Journal of experimental medicineJuly 6, 2020Bodda C, Reinert LS, Fruhwürth S, Richardo T, Sun C, Zhang BC, Kalamvoki M, Pohlmann A, Mogensen TH, Bergström P, et al.for immunoblotting were rabbit anti-STING (D2P2F/#13647, 1:1,000; Cell Signaling), sheep anti-STING (R&D Systems, AF6516, 1:500), rabbit anti-STING (rodent preferred; #50494, 1:1,000; Cell Signaling), rabbit anti-pSTING (S366;

(9) Neutrophil extracellular traps released by neutrophils impair revascularization and vascular remodeling after strokeNature communicationsMay 19, 2020Kang L, Yu H, Yang X, Zhu Y, Bai X, Wang R, Cao Y, Xu H, Luo H, Lu L, et al.-Zonula occludens-1 (ZO-1; 1 : 1000, 617300, Invitrogen), sheep anti-stimulator of IFN genes (STING; 1 : 1000, AF6516, R&D Systems). The membranes were incubated overnight with primary antibodies, followed by incubation

(10) Attenuation of cGAS-STING signaling is mediated by a p62/SQSTM1-dependent autophagy pathway activated by TBK1The EMBO journalApril 13, 2018Prabakaran T, Bodda C, Krapp C, Zhang BC, Christensen MH, Sun C, Reinert L, Cai Y, Jensen SB, Skouboe MK, et al.1:1,000), rabbit antiSTING (Cell Signaling, D2P2F/#13647, 1:1,000), sheep anti-STING (R&D Systems, AF6516, 1:500), rabbit anti-pSTING (S366) (Cell Signaling Technology, #85735), rabbit anti-TBK1 (Cell Signaling, D1B4/#3504,

(11) IFI16 is required for DNA sensing in human macrophages by promoting production and function of cGAMPNature communicationsFebruary 10, 2017Jønsson KL, Laustsen A, Krapp C, Skipper KA, Thavachelvam K, Hotter D, Egedal JH, Kjolby M, Mohammadi P, Prabakaran T, et al.were stained with antibodies directed against IFI16 (C-18, Santa Cruz sc-6050), STING (R&D Systems, AF6516) or IRF3 (Cell Signaling 4302, s). Secondary antibodies included Alexa Fluor 488 Donkey-anti-rabbit,

(12) cGAS Senses Human Cytomegalovirus and Induces Type I Interferon Responses in Human Monocyte-Derived CellsPLoS pathogensApril 1, 2016Paijo J, Döring M, Spanier J, Grabski E, Nooruzzaman M, Schmidt T, Witte G, Messerle M, Hornung V, Kaever V, et al.

(13) "Listeria monocytogenes induces IFNβ expression through an IFI16-, cGAS- and STING-dependent pathway"The EMBO journalJanuary 1, 0001"Hansen K, Prabakaran T, Laustsen A, Jørgensen SE, Rahbæk SH, Jensen SB, Nielsen R, Leber JH, Decker T, Horan KA, et al."The antibodies used were rabbit anti-cGAS (HPA031700 SIGMA, AntiMB21D1) and sheep anti-STING (R&D Systems, AF6516). Images were acquired on Zeiss LSM 710 confocal microscope, using a 63× 1.4 oil lens. Image processing

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