Immunocytochemistry/Immunofluorescence analysis of UAP1 in U-2 OS cells using UAP1 Recombinant Monoclonal Antibody (Product # MA5-38172) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of UAP1 in extracts of various cell lines. Samples were incubated with UAP1 Monoclonal antibody (Product # MA5-38172) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.