Western Blot using Transferrin Receptor Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41056) at 1:500 dilution. Lane A: Transferrin Receptor konckout Hela Whole Cell Lysate, Lane B: Hela Whole Cell Lysate. Lysates/proteins at 20 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 84 kDa. Observed band size: 85 kDa.

Transferrin Receptor Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg MCF-7 Whole Cell Lysate, Lane C: 0.5 mg HepG2 Whole Cell Lysate, Lane D: 0.5 mg Hela Whole Cell Lysate 2 µL with Transferrin Receptor Recombinant Rabbit Monoclonal Antibody (001) (Product # MA5-41056) and 15 µL of 50 % Protein G agarose. Primary antibody: Transferrin Receptor Recombinant Rabbit Monoclonal Antibody (001), at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 84 kDa. Observed band size: 100 kDa.