GATA-6 (D61E4) XP ® Rabbit mAb 5851S from Cell Signaling Technology

Product Specs
ItemGATA-6 (D61E4) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number5851S
Quantity100 µl
ApplicationsW, W-S, IHC-P, IF-IC, FC-FP, ChIP, ChIP-seq, C&R, C&T
ReactivityH, M
TargetGATA6
HostRabbit
ClonalityMonoclonal
NCBI Full Gene NameGATA binding protein 6
Research AreaDevelopmental Biology
IsotypeIgG
CloneD61E4
Molecular Weight55

Questions and Answers

What role does GATA6 play in embryonic stem cell differentiation?

GATA6 is a crucial transcription factor involved in the differentiation of embryonic stem cells into endodermal lineages. It has been shown to regulate genes that are essential for pancreatic development and is implicated in the differentiation processes of human induced pluripotent stem cells, particularly in the context of cardiac and pancreatic lineage specification ().

Has GATA6 been studied in the context of cancer research?

Yes, GATA6 has been linked to pancreatic ductal adenocarcinoma through its role in pancreatic differentiation. Research has demonstrated that GATA6 is involved in the transcriptional regulation of genes necessary for pancreatic development, and its expression is critical for maintaining the identity of pancreatic progenitor cells ().

How does GATA6 expression affect the immune response in the context of chemotherapy?

GATA6 expression is associated with the phenotype of resident peritoneal macrophages, which play a significant role in the immune response. Studies have shown that GATA6+ macrophages are depleted following treatment with the chemotherapeutic agent CPT-11, indicating that GATA6 is important for the maintenance and recovery of these immune cells after chemotherapy-induced damage ().

Are there any known mutations in GATA6 that affect development?

Yes, damaging mutations in GATA6 have been associated with various developmental defects, including cardiac outflow tract defects and malformations of the pancreas and diaphragm. These mutations can disrupt the normal transcriptional and epigenetic regulation during differentiation, leading to significant developmental abnormalities ().

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(1) ALDH1A3 promotes the lung metastatic organotropism of pancreatic ductal adenocarcinoma through semaphorin signalingiScienceJune 19, 2026Shanshan Shen, Xuetian Qian, Zhao Shi, Yuting Wu, Zitong Zhou, Shuang Nie, Yuanyuan Yu, Siqi Zhou, Kun Liu, Quan Zhou, et al.,Cell Signaling Technology,"Cat# 9715, RRID: AB_331563" Rabbit anti-GATA6,Cell Signaling Technology,"Cat# 5851, RRID: AB_10705521" KRT7,, "Goat anti-rabbit IgG, HRP-linked",Cell Signaling Technology,"Cat# 7074,

(2) Nanoparticle-Loaded Injectable Hydrogel Alleviates Titanium Particle-Induced Osteolysis by Disrupting GATA6/DDX3X-Mediated Macrophage InflammationBiomaterials ResearchApril 16, 2026Sipeng Lin, Taihe Liu, Qi Zhu, Zhuji Ouyang, Yifan Yu, Haopeng Sun, Changchuan Li, Shixun Li, Chenhao Pan, Wing Cheuk Ko, et al.8570, Cell Signaling Technology), FIS1 (Rabbit, 32525, Cell Signaling Technology), and GATA6 (Rabbit, 5851, Cell Signaling Technology). RNA-seq Total RNA was extracted from the Trizol reagent kit (Invitrogen,

(3) Single-cell ATAC-seq analysis of human embryoid bodies reveals crucial transcription factor networks involved in early germ layer specificationCell & BioscienceMarch 29, 2026Linying Li, Xiaoyu He, Feng Zhang, Xiaodong Cai, Qiuhui Zhang, Tie Yang, Ran Tong, Shuang Li, Xiaoyan Ding, Yang Dong, et al.1:350); anti-SOX1 (Abcam ab109290, 1:100); anti-TBR2 / Eomes (Abcam ab216870, 1:100); anti-GATA6 (CST 5851, 1:1600); anti-SOX17 (Abcam ab224637,1:500). Secondary antibodies conjugated to either Alexa Fluor 488

(4) Molecular and epistatic interactions between pioneer transcription factors shape nucleosome dynamics and cell differentiationNature CommunicationsDecember 10, 2025Rémi-Xavier Coux, Agnès Dubois, Almira Chervova, Inma Gonzalez, Sandrine Vandormael-Pournin, Michel Cohen-Tannoudji, Pablo Navarro°C overnight rotating on-wheel with the following antibodies: anti-GATA6 (Cell Signaling technology #5851); anti-OCT4 (abcam ab19857); anti-SOX2 (Active Motif 39823); anti-ESRRB (Perseus Proteomics, H6-705-00);

(5) RepID promotes metastatic potential in osteosarcoma through regulation of the PRC1-GATA6 axisAnim Cells Syst (Seoul)December 8, 2025Dong-Hyun Jung, Jong-Uk Park, Jae-Hyun Jo, Dong-Kyu Kim, Yeong-Mu Kim, Min-Ju Kang, Suk-Hoon Hong, Hyo Je Cho, Jihoon Nah, Jun-Young Park, et al.(Cell Signaling, 13255, 1:2,000), anti-CDH1 (Cell Signaling, 14472, 1:2,000), anti-GATA6 (Cell Signaling, 5851, 1:1,000), anti-CDH2 (Cell Signaling, 13116, 1:2,000), anti-RepID (Abcam, ab86244, 1:1,000), anti-SLUG

(6) Stage-sensitive potential of isolated rabbit ICM to differentiate into extraembryonic lineages†Biology of ReproductionNovember 14, 2025Katarzyna Filimonow, Anna Chołoniewska, Jan Chołoniewski, Zofia E Madeja, Katarzyna Barłowska, Joanna Grabarek, Elżbieta Wenta-Muchalska, Berenika Plusa, Anna Piliszek,R&D systems,AF1924,AB_355060 GATA6,Goat,R&D systems,AF1700,AB_2108901 GATA6,Rabbit,Cell Signaling technology,5851,AB_10705521 List of primary antibodies used.

(7) Depletion of Sorcs3 Activates Totipotency in Mouse Embryonic Stem Cells by Modulating Key Signaling PathwaysAdvanced ScienceNovember 3, 2025Wenhao Zhang, Xinyu Mao, Yu He, Qingshen Jia, Yiding Zhao, Xiaomeng Dai, Xiaoyan Li, Shengyi Sun, Xiaoyan Sheng, Dan Ding, et al.(BioGenex, MU392A), anti‐EOMES (Abcam, ab183991), anti‐TFAP2C (Santa Cruz, sc‐12762), anti‐GATA6 (CST, 5851S), anti‐OCT4 (Abcam, ab81557), anti‐TPBPA (Abcam, ab104401), anti‐PROLIFERIN (Santa Cruz, sc‐271891),

(8) Transcription factor switching drives subtype-specific pancreatic cancerNature GeneticsOctober 30, 2025Shalini V. Rao, Lisa Young, Danya Cheeseman, Sean Flynn, Niklas Krebs, Dominique-Laurent Couturier, Stephanie Mack, Rebecca Brais, Jill Temple, Amy Smith, et al.HNF4A (Cell Signaling Technology, 3113S), PRMT1 (Atlas, HPA072136), GATA6 (Cell Signaling Technology, 5851; R&D Systems, AF1700) and FOXA1 (Abcam, ab5089). Beads were washed ten times in RIPA buffer (50 mM,

(9) CRISPR Screening Reveals a Novel Role for FOXH1 in Regulating Pluripotency of Porcine Embryonic Stem CellsAdvanced ScienceSeptember 1, 2025Peng Su, Linhui Wu, Delong Li, Wenting Song, Dagang Tao, Liang Liu, Qi Wang, Manxin Gao, Tian Xu, Xin Liu, et al.1:100; SOX1, CST, 41 945, 1:100; FGF5, Abcam, ab88118, 1:100; S0×17, R&D, AF1924, 1:200; GATA6, CST, 5851, 1:100) overnight at 4 °C. After washed in PBS three times, the cells were incubated with fluorescent‐dye

(10) Epiregulon: Single-cell transcription factor activity inference to predict drug response and drivers of cell statesNat CommunAugust 2, 2025Tomasz Włodarczyk, Aaron Lun, Diana Wu, Minyi Shi, Xiaofen Ye, Shreya Menon, Shushan Toneyan, Kerstin Seidel, Liang Wang, Jenille Tan, et al.#: 004-650) following the standard manufacturer’s protocol. GATA6 (Cell Signaling Technology, Cat #: 5851) and beta-actin antibodies were used at 1:50 dilutions. Incucyte growth assays A total of 3000 LNCaP

(11) Tumor Organoids Grown in Mixed-Composition Hydrogels Recapitulate the Plasticity of Pancreatic CancersGelsJuly 21, 2025Ioritz Sorzabal-Bellido, Xabier Morales, Iván Cortés-Domínguez, Maider Esparza, Lucía Grande, Pedro Castillo, Silvia Larumbe, María Monteserín, Shruthi Narayanan, Mariano Ponz-Sarvise, et al.Paraffin-embedded samples were cut at 5 μm thickness and immunostained for GATA6 (Cell Signaling, Danvers, MA, USA, 5851). Hematoxylin and eosin (H&E) staining was performed using standard protocols to assess tumor area.

(12) E-cadherin negatively regulates hESCs endodermal differentiation under varied substrate stiffnessesCellular and molecular life sciences : CMLSJune 23, 2025Zhang F, Zheng L, Wu Y, Ding Q, Lü S, Lü D, Long M.protein A agarose beads (9863), cell lysis buffer (9803), rabbit IgG isotype control (3900), anti-GATA6 (5851), anti-YAP (12395 and 14074), anti-GAPDH (2118), and anti-mouse IgG conjugated AF647 (4410) antibodies

(13) Protocol for differentiation of human pluripotent stem cells into definitive endoderm lineage in a chemically defined and growth factor-free systemSTAR ProtocJune 20, 2025He En, Yijie Guo, Zhiju Zhao, Xue Huang, Nan Cao, Jia Wang,Santa Cruz Biotechnology,Cat#SC25310; RRID: AB_627667 "GATA-6 (D61E4) XP rabbit mAb, dilution 1:200",Cell Signaling Technology,Cat# 5851; RRID: AB_10705521 "CD184 (CXCR4) monoclonal antibody (12G5), APC, eBioscience",Thermo Fisher Scientific,Cat#17-9999-42;

(14) Self-organization of mouse embryonic stem cells into reproducible pre-gastrulation embryo models via CRISPRa programmingCell Stem CellJune 5, 2025Gerrald A. Lodewijk, Sayaka Kozuki, Clara J. Han, Benjamin R. Topacio, Seungho Lee, Lily Nixon, Abolfazl Zargari, Gavin Knight, Randolph Ashton, Lei S. Qi, et al.,Cell Signaling Technology,Cat#12306; RRID: AB_2797879 Rabbit monoclonal anti-Gata6,Cell Signaling Technology,Cat#5851; RRID: AB_10705521 Rabbit monoclonal anti-Gata3,Cell Signaling Technology,Cat#5852; RRID: AB_10835690 Goat

(15) Proteogenomic reprogramming to a functional human blastomere-like stem cell state via a PARP-DUX4 regulatory axisCell ReportsMay 27, 2025Ludovic Zimmerlin, Ariana Angarita, Tea Soon Park, Rebecca Evans-Moses, Justin Thomas, Sirui Yan, Isabel Uribe, Isabella Vegas, Clara Kochendoerfer, Willem Buys, et al.,Cell Signaling Technology,Cat#36966; clone D3A3M Rabbit monoclonal anti-GATA6,Cell Signaling Technology,Cat#5851; clone D61E4 Rabbit polyclonal anti-HOXA13,ThermoFisher Scientific - Invitrogen,Cat#PA5–76440; RRID:

(16) HMGA2 and protein leucine methylation drive pancreatic cancer lineage plasticityNat CommunMay 26, 2025Stephanie Dobersch, Naomi Yamamoto, Aidan Schutter, Sarah M. Cavender, Tess M. Robertson, Nithya Kartha, Annie N. Samraj, Ben Doron, Lisa A. Poole, Cynthia L. Wladyka, et al.(LS Bio, #LS-B3423) 1:400, anti-HMGA2 (CST #5269) 1:100, anti-p53 (Vector VP-P956) 1:400, anti-GATA6 (CST #5851S) 1:100, and anti-phospho-S6K (Abcam # ab2571) 1:100 for human tissues. Stained slides were photographed

(17) Loss of GATA2 promotes invasion and predicts cancer recurrence and survival in uterine serous carcinomaJCI InsightMay 8, 2025Usha S. Polaki, Trey E. Gilpin, Apoorva T. Patil, Emily Chiu, Ruth Baker, Peng Liu, Tatiana S. Pavletich, Morteza Seifi, Paula M. Mañán-Mejías, Jordan Morrissey, et al.(Supplemental Figure 2). GATA6 IHC was performed using a commercial antibody (Cell Signaling Technology, 5851). Briefly, deparaffinization was carried out on the instrument, as was heat-induced epitope retrieval

(18) A spatiotemporal and machine-learning platform facilitates the manufacturing of hPSC-derived esophageal mucosaDevelopmental CellMay 1, 2025Ying Yang, Carmel Grace McCullough, Lucas Seninge, Lihao Guo, Woo-Joo Kwon, Yongchun Zhang, Nancy Yanzhe Li, Sadhana Gaddam, Cory Pan, Hanson Zhen, et al.anti-KRT5,BioLegend,Cat# 905904; RRID: AB_2721743 Rabbit monoclonal anti-GATA6,Cell Signaling,Cat# 5851; RRID: AB_10705521 Goat polyclonal anti-NCAM1,R&D systems,Cat# AF2408; RRID: AB_442152 Mouse monoclonal

(19) Treatment of Acute Liver Injury through Selective Tropism of High Mobility Group Box 1 Gene-Silenced Large Peritoneal MacrophagesACS NanoApril 1, 2025Dhaval Oza, Fernando Ivich, Kirsten Deprey, Kelsey Bittner, Keith Bailey, Sarah Goldman, Mikyung Yu, Mark Niedre, Ho-Chou Tu, Mansoor M. AmijiBiocare Medical) and peroxidase inhibitor before the application of the primary antibody (Gata6, Cell Signaling #5851S) at a 1:50 dilution for 1 h. After primary antibody incubation, an antirabbit secondary was applied

(20) GATA6 regulates WNT and BMP programs to pattern precardiac mesoderm during the earliest stages of human cardiogenesisElifeMarch 13, 2025Joseph A Bisson, Miriam Gordillo, Ritu Kumar, Neranjan de Silva, Ellen Yang, Kelly M Banks, Zhong-Dong Shi, Kihyun Lee, Dapeng Yang, Wendy K Chung, et al.using cells at day 2 of cardiac differentiation. 7 μg of the GATA6 antibody (Cell Signaling Technology 5851) was used to cross-link to the Protein A/G Magnetic beads and 1 mg of protein was used for the IP reaction

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12/21/2018Great Antibody to Detect GATA-6 ExpressionDr. Min Hee OhWe study tissue-resident macrophages. To define a specific subset of peritoneal macrophages, we analyze expression of the transcription factor GATA-6 as a marker for this population by immunoblotting.... Read Review

GATA-6 (D61E4) XP ® Rabbit mAb

GATA-6 (D61E4) XP ® Rabbit mAb from Cell Signaling Technology

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GATA-6 (D61E4) XP ® Rabbit mAb from Cell Signaling Technology

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