EMMPRIN/CD147 Antibody AF972 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemEMMPRIN/CD147 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $539.00Supplier Page View Company Product Page
Catalog NumberAF972
NCBI Gene AliasesEMPRIN, OK, TCSF, CD147, HAb18G, EMMPRIN, 5F7
ApplicationsELISA Development,ELISA,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Simple Western,Immunocytochemistry, ELISA Capture
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
HostGoat
TargetEMMPRIN/CD147
ReactivityHuman,Mouse
NCBI Full Gene Namebasigin (Ok blood group)
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147, Thr25-His205, Accession # Q54A51
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ValidationKnockout Validated

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse EMMPRIN is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Dihydrotestosterone Augments the Angiogenic and Migratory Potential of Human Endothelial Progenitor Cells by an Androgen Receptor-Dependent MechanismInternational Journal of Molecular SciencesApril 29, 2024Mirel Adrian Popa, Cristina Maria Mihai, Viorel Iulian Șuică, Felicia Antohe, Raghvendra K. Dubey, Brigitte Leeners, Maya Simionescuanti-human: ab39256 (Abcam, Cambridge, UK); PlGF anti-human: ab196666 (Abcam); and EMMPRIN anti-human: AF972 (R&D Systems, Minneapolis, MN, USA). Then, the membranes were washed with TBS and incubated with the

(2) Potential protective effects of breast milk and amniotic fluid against novel coronavirus SARS-CoV-2 through decoy receptorsPediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and ImmunologyJanuary 1, 2022Rees A, Turner S, Thornton CA.significant. Immunoblotting analysis (ACE2 (15983), CD26 (67138), Cell Signaling Technologies; CD147 (AF972), Bio‐Techne; NRP‐1 (ab81321), Abcam) was used to confirm findings from the ELISAs and reveal different

(3) Dysbalance of ACE2 levels - a possible cause for severe COVID-19 outcome in COPDThe journal of pathology. Clinical researchSeptember 1, 2021Fließer E, Birnhuber A, Marsh LM, Gschwandtner E, Klepetko W, Olschewski H, Kwapiszewska G.Cell Signaling Technology, Boston, MA, USA), Furin (1:100, #sc‐133 142; Santa Cruz, Dallas, TX, USA), or CD147 (1:100, #AF972; R&D Systems, Minneapolis, MN, USA) at 4 °C overnight. The primary antibodies were detected with the

(4) Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoringNature communicationsSeptember 7, 2020Sun N, Lee YT, Zhang RY, Kao R, Teng PC, Yang Y, Yang P, Wang JJ, Smalley M, Chen PJ, et al.

(5) CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblastsBMC cancerSeptember 12, 2019Aoki M, Koga K, Miyazaki M, Hamasaki M, Koshikawa N, Oyama M, Kozuka-Hata H, Seiki M, Toole BP, Nabeshima K.

(6) Efficacy Evaluation of Combination Treatment Using Gemcitabine and Radioimmunotherapy with ⁹⁰Y-Labeled Fully Human Anti-CD147 Monoclonal Antibody 059-053 in a BxPC-3 Xenograft Mouse Model of Refractory Pancreatic CancerInternational journal of molecular sciencesSeptember 29, 2018Sugyo A, Tsuji AB, Sudo H, Koizumi M, Ukai Y, Kurosawa G, Kurosawa Y, Saga T, Higashi T.used as a control. Sections (1-µm thick) were immunostained with a goat anti-EMMPRIN/CD147 antibody (AF972, R&D Systems, Minneapolis, MN, USA, diluted 1:500) as described previously [15]. MMP2 and MMP9 were

(7) Structure of the human plasma membrane Ca²⁺-ATPase 1 in complex with its obligatory subunit neuroplastinNature communicationsSeptember 6, 2018Gong D, Chi X, Ren K, Huang G, Zhou G, Yan N, Lei J, Zhou Q.

(8) Dihydrotestosterone induces pro-angiogenic factors and assists homing of MSC into the cardiac tissueJournal of molecular endocrinologyJanuary 1, 2018Popa MA, Mihai MC, Constantin A, Şuică V, Ţucureanu C, Costache R, Antohe F, Dubey RK, Simionescu M.(Sigma-Aldrich Chemie GmbH); anti-human VEGF: MAB293R, anti-human Angiogenin: AF265, and anti-human EMMPRIN: AF972 (R&D Systems). After TBS washings, the membranes were incubated with the corresponding HRP-conjugated

(9) Overexpression of CD147 is associated with poor prognosis, tumor cell migration and ERK signaling pathway activation in hepatocellular carcinomaExperimental and therapeutic medicineSeptember 1, 2017Xiao W, Zhao S, Shen F, Liang J, Chen J.serum at 37˚C for 20 min. Slices were then incubated with anti‑EMMPRIN/CD147 antibody (catalogue no. AF972; R&D Systems; 5 µg/ml) overnight at 4˚C, followed by incubation at 37˚C for 30 min with biotin‑labeled

(10) Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf TherapyScientific reportsMay 23, 2016Gramlich OW, Burand AJ, Brown AJ, Deutsch RJ, Kuehn MH, Ankrum JA.

(11) CD147 and Ki-67 overexpression confers poor prognosis in squamous cell carcinoma of oral tongue: a tissue microarray studyOral surgery, oral medicine, oral pathology and oral radiologyMay 1, 2015Yu YH, Morales J, Feng L, Lee JJ, El-Naggar AK, Vigneswaran N.Ig 1:200 eBioscience Inc, San Diego, CA, USA (BMS 124) CD147 Goat Ig 1:200 R & D Systems, Minneapolis, MN, USA AF972 Glut-1 Rabbit Ig 1:250 Thermo Scientific. Waltham, MA, USA RB-9052 Author Manuscript Author Manuscript Oral

(12) Evaluation of (89)Zr-labeled human anti-CD147 monoclonal antibody as a positron emission tomography probe in a mouse model of pancreatic cancerPloS oneJanuary 1, 2013Sugyo A, Tsuji AB, Sudo H, Nagatsu K, Koizumi M, Ukai Y, Kurosawa G, Zhang MR, Kurosawa Y, Saga T.sections (10 µm thick) were fixed with cold methanol and were immunostained with goat anti-CD147 antibody (AF972, R&D Systems, Minneapolis, MN, USA). Nonspecific binding was blocked by Block Ace regent with 10% goat

(13) Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migrationAmerican journal of physiology. Heart and circulatory physiologyAugust 1, 2009Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B.

(14) Emmprin in epithelioid sarcoma: expression in tumor cell membrane and stimulation of MMP-2 production in tumor-associated fibroblastsInternational journal of cancerFebruary 15, 2007Koga K, Nabeshima K, Aoki M, Kawakami T, Hamasaki M, Toole BP, Nakayama J, Iwasaki H.

(15) Expression of emmprin and matrix metalloproteinases (MMPs) in peripheral nerve sheath tumors: emmprin and membrane-type (MT)1-MMP expressions are associated with malignant potentialAnticancer researchMarch 1, 2006Nabeshima K, Iwasaki H, Nishio J, Koga K, Shishime M, Kikuchi M.

(16) CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin AProceedings of the National Academy of Sciences of the United States of AmericaMay 22, 2001Pushkarsky T, Zybarth G, Dubrovsky L, Yurchenko V, Tang H, Guo H, Toole B, Sherry B, Bukrinsky M.

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EMMPRIN/CD147 Antibody

EMMPRIN/CD147 Antibody

EMMPRIN/CD147 Antibody

EMMPRIN/CD147 Antibody

EMMPRIN/CD147 Antibody

EMMPRIN/CD147 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (16)

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EMMPRIN/CD147 Antibody from R&D Systems, a Bio-Techne Brand

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