Mouse IGF-II/IGF2 Antibody AF792 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse IGF-II/IGF2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF792
Quantity100 ug (also 25 ug)
ApplicationsImmunohistochemistry,Western Blot,Immunohistochemistry-Frozen,Neutralization
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ConcentrationLYOPH
PurityAntigen Affinity-purified
ReactivityMouse,Transgenic Mouse
IsotypeIgG
TargetIGF-II/IGF2
HostGoat
Antibody TypePrimary Antibodies
NCBI Gene AliasesM6pr, Mpr, Peg2, Igf-2, Igf-II
NCBI Full Gene Nameinsulin-like growth factor 2
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant mouse IGF-II/IGF2, Ala25-Glu91, Accession # P09535
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

What applications has the IGF-II/IGF2 antibody been used for in scientific research?

The IGF-II/IGF2 antibody has been shown to work in applications such as Western blotting and immunohistochemistry. It has been utilized to study the protective role of IGF-2 in motor neurons in models of amyotrophic lateral sclerosis, as demonstrated in the research by Allodi et al. (2016), where IGF-2 was found to protect motor neurons from degeneration.

Can this antibody be used to detect IGF-2 in different experimental setups?

Yes, the IGF-II/IGF2 antibody has been demonstrated to detect mouse IGF-II in direct ELISAs and Western blots. Additionally, it has been cited in studies involving immunohistochemistry, where it was used to analyze IGF-2 expression in various tissues, including muscle and neuronal tissues.

Is there any information on the specificity of the IGF-II/IGF2 antibody regarding cross-reactivity with human IGF-II?

The antibody exhibits approximately 50% cross-reactivity with recombinant human IGF-II in direct ELISAs, which suggests that it can be used in studies involving both mouse and human IGF-II, although it is primarily designed for mouse applications.

How has the IGF-II/IGF2 antibody contributed to understanding muscle differentiation and regeneration?

The IGF-II/IGF2 antibody has been used in studies that explore the role of IGF-2 in muscle differentiation. For instance, research has shown that IGF-2 is crucial for muscle cell differentiation and that its expression can be regulated by factors such as Lin-28, which enhances the translation of IGF-2 mRNA during myogenesis (Polesskaya et al., 2007).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) IGF2 supports glioblastoma growth and immune evasion through a combination of tumor cell-intrinsic and -extrinsic mechanismsNeuro-Oncology AdvancesJanuary 12, 2026Kyle M Heemskerk, Samir Assaf, Xiaoguang Hao, Shannon Snelling, Mathieu Meode, Rozina Hassam, Orsolya Cseh, Smriti Kala, James Pemberton, Jennifer A Chan, et al.sonication. Blots were probed overnight at 4°C with antibodies specific to mouse IGF2 (R&D Systems—AF792) and β-ACTIN (Sigma-A5316) as a loading control. Blots were washed and incubated with HRP-conjugated

(2) Overexpression of Igf2-derived Mir483 inhibits Igf1 expression and leads to developmental growth restriction and metabolic dysfunction in miceCell reportsSeptember 24, 2024Sandovici I, Fernandez-Twinn DS, Campbell N, Cooper WN, Sekita Y, Zvetkova I, Ferland-McCollough D, Prosser HM, Oyama LM, Pantaleão LC, et al.

(3) Activation of Proneuronal Transcription Factor Ascl1 in Maternal Liver Ensures a Healthy PregnancyCellular and molecular gastroenterology and hepatologyJanuary 1, 2022Lee J, Garcia V, Nambiar SM, Jiang H, Dai G.immunohistochemistry. Primary antibodies against β-catenin (610153, 1:100; BD Transduction, San Jose, CA), IGF2 (AF792, 1:1000; R&D Systems, Minneapolis, MN), and Ki67 (RM-9106-S1, 1:100; Thermo Fisher Scientific, Waltham,

(4) Lacrimal gland budding requires PI3K-dependent suppression of EGF signalingScience advancesJune 1, 2021Wang Q, Tao C, Hannan A, Yoon S, Min X, Peregrin J, Qu X, Li H, Yu H, Zhao J, et al.(PerkinElmer Life Sciences, Waltham, MA). Antibodies used are Egfr (ab52894) (Abcam); IGF1 (AF791), IGF2 (AF792), IGF1R (AF305), and IGF2R (NB300-514) (Novus Biologicals); FGFR2 (#23328), p4EBP1 (#2855), p85 (#4257),

(5) Mononuclear diploid cardiomyocytes support neonatal mouse heart regeneration in response to paracrine IGF2 signalingeLifeMarch 13, 2020Shen H, Gan P, Wang K, Darehzereshki A, Wang K, Kumar SR, Lien CL, Patterson M, Tao G, Sucov HM.(goat polyclonal),Santa Cruz,SC8697,for IF (1:200) Antibody,anti-IGF2 (goat polyclonal),R&D Systems,AF792,for Westerns (1:1000) Antibody,anti-phospho-IGF1R/INSR (rabbit polyclonal),Invitrogen,700393,for Westerns;

(6) Maternal transmission of an Igf2r domain 11: IGF2 binding mutant allele (Igf2r(I1565A)) results in partial lethality, overgrowth and intestinal adenoma progressionScientific reportsAugust 6, 2019Hughes J, Surakhy M, Can S, Ducker M, Davies N, Szele F, Bühnemann C, Carter E, Trikin R, Crump MP, et al.blots incubated with Ponceau S to verify equal protein loading. For detection, anti-IGF2 (R&D systems, AF792) at 0.4 µg ml−1 was used as the primary antiobody with an HRP-conjugated secondary antibody (Dako, 1:2000). Immunofluorescence Tissue

(7) A CCR2+ myeloid cell niche required for pancreatic β cell growthJCI insightAugust 3, 2017Mussar K, Pardike S, Hohl TM, Hardiman G, Cirulli V, Crisa L.

(8) Differential neuronal vulnerability identifies IGF-2 as a protective factor in ALSScientific reportsMay 16, 2016Allodi I, Comley L, Nichterwitz S, Nizzardo M, Simone C, Benitez JA, Cao M, Corti S, Hedlund E.(SAB4504331),Rabbit,–,–,–,1:200,– β-catenin,Santa Cruz (E-5),Mouse,–,–,–,1:200,– HB9,Millipore (ABN174),Rabbit,–,–,–,1:200,– IGF-2,R&D Systems (AF792),Goat,1:40,–,1:20,–,– IGF-2,Abcam (ab9574),Rabbit,1:200,–,1:100,–,– IGF-1 receptor,R&D Systems (AF-305-NA),Goat,1:40,1:20,–,–,– pIGF-1

(9) Maternal transmission of a humanised Igf2r allele results in an Igf2 dependent hypomorphic and non-viable growth phenotypePloS oneJanuary 1, 2013Hughes J, Frago S, Bühnemann C, Carter EJ, Hassan AB.primary antibodies in blocking buffer containing 5% milk. Antibodies used were anti-IGF2 (R&D systems, AF792) at 0.4 µg ml− 1, anti-IGF2R (R&D systems, AF2447) at 0.1 µg ml− 1, anti-Cathepsin L (R&D systems, AF1515)

(10) Induction of a regenerative microenvironment in skeletal muscle is sufficient to induce embryonal rhabdomyosarcoma in p53-deficient miceThe Journal of pathologyJanuary 1, 2012Camboni M, Hammond S, Martin LT, Martin PT.were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibody to Igf1 (AF791) and Igf2 (AF792) were purchased from R&D systems (Minneapolis, MN, USA). All secondary reagents were purchased from

(11) Glucose regulated protein 94 is required for muscle differentiation through its control of the autocrine production of insulin-like growth factorsBiochimica et biophysica actaFebruary 1, 2010Ostrovsky O, Eletto D, Makarewich C, Barton ER, Argon Y.Biotinylated anti-mouse IGF-II (#BAF792), monoclonal anti-IGF-II (MAB792) and polyclonal anti-mouse IGF-II (AF792) were from R&D Systems (Minneapolis, MN). The anti-GRP94 monoclonal antibody 9G10 (SPA-850) from Stressgen

(12) Mice lacking dystrophin or alpha sarcoglycan spontaneously develop embryonal rhabdomyosarcoma with cancer-associated p53 mutations and alternatively spliced or mutant Mdm2 transcriptsThe American journal of pathologyJanuary 1, 2010Fernandez K, Serinagaoglu Y, Hammond S, Martin LT, Martin PT.inhibiting factor (AF 1457, rabbit pAb to human aa121-613), Igf1 (AF791, goat IgG to mouse IGF-1), Igf2 (AF792, goat IgG to mouse IGF-II), and actin (AF4000, sheep pAb to human actin) were purchased from R&D Systems

(13) The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivationMolecular biology of the cellMarch 1, 2009Ostrovsky O, Ahmed NT, Argon Y.biotinylated antimouse IGF-II (BAF792), monoclonal anti-IGF-II (MAB792), and polyclonal anti-mouse IGF-II (AF792) were from R&D Systems; and antibodies against cleaved caspase 3 (FITC-conjugated; no. 9667 and 9661)

(14) Role of IGF signaling in olfactory sensory map formation and axon guidanceNeuronMarch 27, 2008Scolnick JA, Cui K, Duggan CD, Xuan S, Yuan XB, Efstratiadis A, Ngai J.

(15) GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretionMolecular biology of the cellOctober 1, 2007Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y.

(16) Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiencyGenes & DevelopmentMay 1, 2007Anna Polesskaya, Sylvain Cuvellier, Irina Naguibneva, Arnaud Duquet, Eric G. Moss, Annick Harel-Bellan(positive control). Antibodies used were (IGF-2*) S1F2 (Upstate Biotechnology), 10 µg/mL; (IGF-2**) AF792 (R&D Systems), 5 µg/mL; (irr.) irrelevant, mixture of antibodies isotype-matched to the IGF-2 antibodies.

(17) Hypoxia-independent overexpression of hypoxia-inducible factor 1alpha as an early change in mouse hepatocarcinogenesisCancer researchDecember 1, 2006Tanaka H, Yamamoto M, Hashimoto N, Miyakoshi M, Tamakawa S, Yoshie M, Tokusashi Y, Yokoyama K, Yaginuma Y, Ogawa K.

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IGF-II/IGF2 Antibody

IGF-II/IGF2 Antibody

IGF-II/IGF2 Antibody

IGF-II/IGF2 Antibody

Mouse IGF-II/IGF2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (17)

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Mouse IGF-II/IGF2 Antibody from R&D Systems, a Bio-Techne Brand

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