IL-10 Antibody AF519 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemIL-10 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF519
NCBI Gene AliasesIL10A, GVHDS, TGIF, IL-10, CSIF, IL10X, If2a
ApplicationsFluorescence Microscopy,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Neutralization,Immunocytochemistry
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
ReactivityMouse,Rat
TargetIL-10
HostGoat
NCBI Full Gene Nameinterleukin 10
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant rat IL-10, Ser19-Asn178, Accession # P29456
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse, rat, and cotton rat IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-10, recombinant guinea pig IL-10, recombinant viral IL-10 and recombinant canine IL-10 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) EXPRESS: Study on the Role and Mechanism of the IL-10-IRF-8 Signaling Pathway in Bone Marrow Mesenchymal Stem Cell Therapy for Pain in CCD RatsMolecular PainMarch 1, 2026Yican Yang, Mutong Wang, Shuai Guo, Yan Feng, Shou-Wei Zhang, Shouwei Zhangμl LV-IL-10-transfected BMSCs (1 × 106 cells) in combination with 20 μl IL-10 antibody (R&D Systems, AF519, USA). 10 To assess whether IL-10 exerts analgesic effects by downregulating IRF-8, 24 rats were randomly

(2) Engineering a streamlined virus-like particle for programmable tissue-specific gene deliveryNature communicationsOctober 15, 2025Han J, Bai H, Li F, Zhang Y, Zhou Q, Li W.ab233706) were 1:500 diluted, anti-CD31 antibody (Servicebio, GB15063-100) and anti-IL-10 (R&D Systems, AF519) was 1:200 diluted, anti-IL-1β antibody (H-153) (Santa Cruz Biotechnology, sc-7884) was 1:100 diluted

(3) Oral delivery of liquid mRNA therapeutics by an engineered capsule for treatment of preclinical intestinal diseaseScience Translational MedicineJuly 16, 2025Xiangang Huang, Chuang Liu, Shonit Nair Sharma, Xinru You, Shuying Chen, Yongjiang Li, Hai-Jun Liu, Bin Liu, Qimanguli Saiding, Wei Chen, et al.immunofluorescence and H&E staining of tissues.” Antibodies dilutions: Mouse/Rat IL-10 Antibody (1:40, AF519, R&D Systems), Villin Polyclonal antibody (1:200, 16488-1-AP, Cell Signaling Technology). For protein

(4) Amelioration of Inflammation and Metabolic Blockage in GALC Deficient Mice After Enzyme Replacement Therapy via Extracellular VesiclesInternational journal of nanomedicineJanuary 1, 2025Zelada D, Saldivia N, Samano S, George D, Chaudhari K, Nguyen D, Simchuk D, Van Breemen RB, Givogri MI.anti-MBP (1:600, a gift from Dr Ernesto Bongarzone, University of Illinois at Chicago), anti-IL-10 (1:500, AF519, R&D Systems), Isolectin GS-IB4 Alexa Fluor 488 Conjugated (Invitrogene), anti-NeuN (1:800, D4G40, Cell

(5) Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exerciseThe Journal of biological chemistryDecember 1, 2022Komori T, Morikawa Y.1:2000; catalog no.: AF5868, R&D Systems); goat anti-IL-10 antibody (diluted at 1:1000; catalog no.: AF519, R&D Systems); goat anti-OSM antibody (diluted at 1:1000, R&D Systems); goat anti-CCL2 antibody (diluted

(6) Cellular Sources and Neuroprotective Roles of Interleukin-10 in the Facial Motor Nucleus after AxotomyCellsOctober 9, 2022Runge EM, Setter DO, Iyer AK, Regele EJ, Kennedy FM, Sanders VM, Jones KJ.anti-GFAP (used in perfused WT tissue),Thermo Fisher PA3-16727,1:1000 Goat anti-IL-10,R&D Systems AF519,1:200 "Donkey anti-rabbit 568 (used for GFAP, IBA1)",Abcam ab175470,1:1000 Donkey anti-goat 488 (used

(7) Adenine Nucleotide Translocase 1 Expression Modulates the Immune Response in Ischemic HeartsCellsAugust 19, 2021Yergöz F, Friebel J, Kränkel N, Rauch-Kroehnert U, Schultheiss HP, Landmesser U, Dörner A.(Cat.No.: GWB-BIGCA2, Genway, Biotech INC, San Diego, USA), IL-6 (Cat.No.: AF506), IL-10 (Cat.No.: AF519), and IL-4 (Cat.No.: MAB504, R&D Systems, Wiesbaden-Nordenstadt, Germany), TGFβ1,2,3 (Cat.No.: sc-7892),

(8) Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injuryNature communicationsJuly 9, 2021Bao Z, Liu Y, Chen B, Miao Z, Tu Y, Li C, Chao H, Ye Y, Xu X, Sun G, et al.monoclonal, 1:1000), GAPDH (YI FEI XUE BIOTECH, YFMA0037, mouse monoclonal, 1:1000), Flag (Beyotime, China, AF519, mouse monoclonal, 1:1000), HA (Beyotime, China, AH158, mouse monoclonal, 1:1000), Fbxo10 (Novus, NBP1-91889,

(9) Ginsenoside RG1 enhances the paracrine effects of bone marrow-derived mesenchymal stem cells on radiation induced intestinal injuryAgingDecember 3, 2020Luo Y, Wang B, Liu J, Ma F, Luo D, Zheng Z, Lu Q, Zhou W, Zheng Y, Zhang C, et al.RRID:AB_2212821)), IL-6 (1 μg/ml; (R&D Systems Cat# AF506, RRID:AB_355398)), IL-10 (1 μg/ml; (R&D Systems Cat# AF519, RRID:AB_355408)), or their combinations. Cell counting Kit-8 (CCK-8 test) Cell proliferation was

(10) Inhibition of Serine Protease Activity Protects Against High Fat Diet-Induced Inflammation and Insulin ResistanceScientific reportsFebruary 3, 2020Kuo CS, Chen JS, Lin LY, Schmid-Schönbein GW, Chien S, Huang PH, Chen JW, Lin SJ.

(11) Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/β-endorphin pathwayJournal of neuroinflammationApril 13, 2019Mao XF, Wu HY, Tang XQ, Ali U, Liu H, Wang YX.

(12) PD-1/PD-L1 checkpoint blockade harnesses monocyte-derived macrophages to combat cognitive impairment in a tauopathy mouse modelNature communicationsJanuary 28, 2019Rosenzweig N, Dvir-Szternfeld R, Tsitsou-Kampeli A, Keren-Shaul H, Ben-Yehuda H, Weill-Raynal P, Cahalon L, Kertser A, Baruch K, Amit I, et al.anti-IL1β (1:100, Santa Cruz Biotechnology, SC-7884); goat anti-IL-10 (1:50, R&D Systems, Minneapolis, MN, #AF519); rabbit anti-MSR1 (1:100, GeneTex, #GTX51749). Secondary antibodies included: Cy2/Cy3/Cy5-conjugated

(13) Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision PainEvidence-based complementary and alternative medicine : eCAMJanuary 1, 2019Dai WJ, Sun JL, Li C, Mao W, Huang YK, Zhao ZQ, Zhang YQ, Lü N.1 h at room temperature and then incubated with a mixture of goat anti-IL-10 (1 : 200, R&D System, AF519, USA), rabbit anti-IL-10 RA (1 : 500, Abcam, Ab225820, UK), rabbit anti-Iba-1 (1 : 500, Wako Pure Chemical

(14) Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by NeuroinflammationFrontiers in pharmacologyJanuary 1, 2018Zhao YW, Pan YQ, Tang MM, Lin WJ.Cell Signaling Technology, Danvers, MA, United States. Rat anti-TNF-α No: AF-510-NA and Mouse/Rat anti-IL-10 No: AF519, 0.1 μg/ml, R&D Systems, Minneapolis, MN, United States) and secondary antibody (Goat anti-Rabbit No:

(15) Autocrine Interleukin-10 Mediates Glucagon-Like Peptide-1 Receptor-Induced Spinal Microglial β-Endorphin ExpressionThe Journal of neuroscience : the official journal of the Society for NeuroscienceNovember 29, 2017Wu HY, Tang XQ, Mao XF, Wang YX.

(16) ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain InjuryThe Journal of neuroscience : the official journal of the Society for NeuroscienceMay 3, 2017Yang Y, Liu H, Zhang H, Ye Q, Wang J, Yang B, Mao L, Zhu W, Leak RK, Xiao B, et al.

(17) Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during ColonizationJournal of immunology (Baltimore, Md. : 1950)April 15, 2017Viladomiu M, Bassaganya-Riera J, Tubau-Juni N, Kronsteiner B, Leber A, Philipson CW, Zoccoli-Rodriguez V, Hontecillas R.

(18) Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretionEMBO molecular medicineMarch 1, 2017Cambier L, de Couto G, Ibrahim A, Echavez AK, Valle J, Liu W, Kreke M, Smith RR, Marbán L, Marbán E.

(19) Breaking immune tolerance by targeting Foxp3(+) regulatory T cells mitigates Alzheimer's disease pathologyNature communicationsAugust 18, 2015Baruch K, Rosenzweig N, Kertser A, Deczkowska A, Sharif AM, Spinrad A, Tsitsou-Kampeli A, Sarel A, Cahalon L, Schwartz M.rabbit anti-IBA-1 (1:300, Wako, #019-19741); goat anti-IL-10 (1:20, R&D systems, Minneapolis, MN, #AF519); rat anti-Foxp3 (1:20, eBioscience, #13-5773-80); rabbit anti-CD3 (1:500, Dako, #IS503); mouse anti-ZO-1,

(20) Therapeutic effects of glatiramer acetate and grafted CD115⁺ monocytes in a mouse model of Alzheimer's diseaseBrain : a journal of neurologyAugust 1, 2015Koronyo Y, Salumbides BC, Sheyn J, Pelissier L, Li S, Ljubimov V, Moyseyev M, Daley D, Fuchs DT, Pham M, et al.#598; MBL), rat anti-CD45 mAb clone 30-F11 (1:25; #550539; BD Pharmingen), goat anti-IL10 pAb (1:100; AF519; R&D systems), and goat anti-MMP9 pAb (1:100; AF909; R&D systems). Secondary polyclonal Abs (donkey anti-mouse,

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IL-10 Antibody

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IL-10 Antibody

IL-10 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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IL-10 Antibody from R&D Systems, a Bio-Techne Brand

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