TIM-3 Antibody AF1529 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTIM-3 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1529
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunocytochemistry,CyTOF-ready
TargetTIM-3
Conjugate/TagUnconjugated
ReactivityMouse,Transgenic Mouse
Quantity100 ug (also 25 ug)
HostGoat
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse TIM-3, Leu22-Arg191, Accession # AAL65156
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
NCBI Full Gene Namehepatitis A virus cellular receptor 2
NCBI Gene AliasesCD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3
PurityAntigen Affinity-purified
ConcentrationLYOPH
IsotypeIgG
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects mouse TIM-3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human TIM-3 is observed and less than 5% cross-reactivity with recombinant mouse (rm) TIM-1, rmTIM-2, rmTIM-4, rmTIM-5, rmTIM-6, and rmTIM-7 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Glial TIM-3 Modulates Immune Responses in the Brain Tumor MicroenvironmentCancer ResearchMay 1, 2020Hyung-Seok Kim, Chi Young Chang, Hee Jung Yoon, Ki Sun Kim, Han Seok Koh, Sang Soo Kim, Sang-Jin Lee, Lawrence P. Kane, Eun Jung ParkCD8 (BD Bioscience, #551162, 0.01μg ml ‒1; #563898, 0.01 μg ml ‒1). Goat anti-TIM-3 (R&D Systems, AF1529, 2 g ml ‒1) and alexa647-conjugated secondary antibody (Molecular Probe , #A-21447, 2 g ml ‒1) were

(2) Transcriptomic Hallmarks of Tumor Plasticity and Stromal Interactions in Brain MetastasisCell reportsApril 23, 2019Wingrove E, Liu ZZ, Patel KD, Arnal-Estapé A, Cai WL, Melnick MA, Politi K, Monteiro C, Zhu L, Valiente M, et al.washed in PBS for 15 minutes. IF experiments, except those including anti-mTIM3 antibody (R&D Systems; AF1529), were blocked in 0.1% Triton X-100 (American Bioanalytical; AB02025) and 0.3% bovine serum albumin

(3) CB2 cannabinoid receptors modulate HIF-1α and TIM-3 expression in a hypoxia-ischemia mouse modelEuropean neuropsychopharmacology : the journal of the European College of NeuropsychopharmacologyDecember 1, 2016Kossatz E, Maldonado R, Robledo P.ab16066), anti-Iba-1 (1:500, Wako; cat. no. 019-19741) and anti-TIM-3 (1:500, R&D systems, cat. no. AF1529). The following day, sections were incubated for 2 h at room temperature with Alexa 647 donkey anti-mouse-IR, Alexa

(4) The HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxiaNature communicationsMarch 20, 2015Koh HS, Chang CY, Jeon SB, Yoon HJ, Ahn YH, Kim HS, Kim IH, Jeon SH, Johnson RS, Park EJ.fixed with methanol, washed with PBS-T and incubated at 4 °C with anti-TIM-3 antibody (R&D Systems, AF1529, 1 μg ml−1). TIM-3 promoter assay A 1,517-bp fragment of the mouse TIM-3 promoter (−1,517 to +1 relative

(5) Tim-3 expression defines a novel population of dysfunctional T cells with highly elevated frequencies in progressive HIV-1 infectionJournal of Experimental MedicineNovember 24, 2008R. Brad Jones et al.

(6) TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cellsBloodOctober 1, 2007Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, Dekruyff RH, Saito H, Galli SJ.

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Mouse TIM-3 Antibody

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TIM-3 Antibody

TIM-3 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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TIM-3 Antibody from R&D Systems, a Bio-Techne Brand

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