Western blot analysis of GABRA4 in rat kidney. Samples were incubated with GABRA4 Polyclonal antibody (Product # PA5-109752) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.