Immunohistochemistry analysis of ANKLE2 in paraffin-embedded human lung cancer. Samples were incubated with ANKLE2 Polyclonal antibody (Product # PA5-109389) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western blot analysis of ANKLE2 in U-87MG, Jurkat, HeLa. Samples were incubated in polyclonal ANKLE2 antibody (Product # PA5-109389) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Western blot analysis of ANKLE2 in extracts of various cell lines. Samples were incubated with ANKLE2 Polyclonal antibody (Product # PA5-109389) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Supplier Page from Thermo Fisher Scientific for ANKLE2 Antibody