Western blot was performed using Anti-IKAROS Recombinant Rabbit Monoclonal Antibody (JB50-38) (Product # MA5-34668) and 60, 52 and 40 kDa bands corresponding to IKZF1 was observed across positive cell lines (REH, Daudi, Ramos and Jurkat) tested. Whole cell extracts (30 µg lysate) of Reh (Lane 1), Daudi (Lane 2), Ramos (Lane 3), Jurkat (Lane 4), U-937 (Lane 5), HL-60 (Lane 6), SH-SY5Y (Lane 7) and A-431 (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).