Western blot analysis of G6PC3 in K-562, HeLa, 293T, mouse kidney, mouse heart. Samples were incubated in polyclonal G6PC3 antibody (Product # PA5-109749) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Immunofluorescence analysis of G6PC3 in U2OS cells. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of G6PC3 in extracts of various cell lines. Samples were incubated with G6PC3 Polyclonal antibody (Product # PA5-109749) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.