Western blot analysis of ORP8 in mouse spleen, mouse kidney, rat thymus, rat kidney. Samples were incubated in polyclonal ORP8 antibody (Product # PA5-110065) using a dilution of 1:1000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Western blot analysis of ORP8 in extracts of various cell lines. Samples were incubated with ORP8 Polyclonal antibody (Product # PA5-110065) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.