Coagulation Factor III/Tissue Factor Antibody AF2339 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCoagulation Factor III/Tissue Factor Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF2339
NCBI Gene AliasesTF, TFA, CD142
ApplicationsELISA Development,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Electron Microscopy,Immunocytochemistry,CyTOF-ready,ELISA Capture
Quantity100 ug (also 25 ug)
IsotypeIgG
ReactivityHuman
Conjugate/TagUnconjugated
TargetCoagulation Factor III/Tissue Factor
HostGoat
NCBI Full Gene Namecoagulation factor III, tissue factor
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived recombinant human Coagulation Factor III/Tissue Factor, Gly34-Glu251, Accession # P13726
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human Coagulation Factor III/Tissue Factor in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this antibody be used to study tissue factor expression in human adipocytes?

Yes, the Coagulation Factor III/Tissue Factor Antibody has been shown to effectively detect tissue factor expression in human primary adipocytes as well as in differentiated 3T3-L1 adipocytes using Western blot analysis, as demonstrated in the study by Edén et al. ().

Is this antibody suitable for use in immunofluorescence applications?

The Coagulation Factor III/Tissue Factor Antibody has been utilized in immunofluorescence staining, as indicated in studies where it was applied to visualize tissue factor in various cell types, including in a vascular injury-on-a-chip model ().

How does this antibody perform in detecting tissue factor in plasma samples?

The Coagulation Factor III/Tissue Factor Antibody has been employed in Western blotting to measure tissue factor levels in plasma, particularly in studies evaluating extracellular vesicles and their procoagulant activity ().

What is the specificity of this antibody for human tissue factor compared to mouse tissue factor?

The antibody demonstrates less than 5% cross-reactivity with recombinant mouse Coagulation Factor III/Tissue Factor, indicating a high specificity for human tissue factor in applications such as direct ELISAs and Western blots.

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) The Proteome of Extracellular Vesicles Released from Pulmonary Microvascular Endothelium Reveals Impact of Oxygen Conditions on BiotraumaInternational journal of molecular sciencesFebruary 19, 2024Schaubmayr W, Hochreiter B, Hunyadi-Gulyas E, Riegler L, Schmidt K, Tiboldi A, Moser B, Klein KU, Krenn K, Scharbert G, et al.

(2) SARS-CoV-2 infection of human lung epithelial cells induces TMPRSS-mediated acute fibrin depositionNature communicationsOctober 11, 2023Erickson R, Huang C, Allen C, Ireland J, Roth G, Zou Z, Lu J, Lafont BAP, Garza NL, Brumbaugh B, et al.secondary antibodies used were polyclonal goat anti-human tissue factor antibody (R&D Systems, inc. Catalog AF2339) at 1:400 dilution and HRP-conjugated rabbit anti-Goat IgG secondary antibody at 1:2000 dilution (R&D

(3) Evaluation of four commercial ELISAs to measure tissue factor in human plasmaResearch and practice in thrombosis and haemostasisMarch 1, 2023Sachetto ATA, Archibald SJ, Bhatia R, Monroe D, Hisada Y, Mackman N.with an anti-TF antibody as the primary antibody (human anti-TF produced in goat, R&D Systems, Cat. #AF2339, 1:1000 in blocking buffer) and subsequently with an antigoat IgG-HRP as a secondary antibody (antigoat

(4) Mechanical Compression of Human Airway Epithelial Cells Induces Release of Extracellular Vesicles Containing Tenascin CCellsJanuary 13, 2022Mwase C, Phung TN, O'Sullivan MJ, Mitchel JA, De Marzio M, Kılıç A, Weiss ST, Fredberg JJ, Park JA.EV markers [13,44,50,57,58,59], including CD9 (D801A; Cell Signaling Technology) and tissue factor (AF2339; R&D Systems, Minneapolis, MN, USA). 2.9. Statistical Analysis For each condition, the results are

(5) PAR2-Induced Tissue Factor Synthesis by Primary Cultures of Human Kidney Tubular Epithelial Cells Is Modified by Glucose AvailabilityInternational journal of molecular sciencesJuly 14, 2021Humphries TLR, Shen K, Iyer A, Johnson DW, Gobe GC, Nikolic-Paterson D, Fairlie DP, Vesey DA.University of Queensland as previously described [52,53]. The human TF antibody from R&D Systems (Cat #AF2339) was used at a dilution of 1/2000. The horseradish peroxidase (HRP)-linked secondary antibodies were

(6) A Human Vascular Injury-on-a-Chip Model of HemostasisSmall (Weinheim an der Bergstrasse, Germany)April 1, 2021Poventud-Fuentes I, Kwon KW, Seo J, Tomaiuolo M, Stalker TJ, Brass LF, Huh D.Peprotech (300–01A). Human coagulation factor III/tissue factor antibody was purchased from R&D Systems (AF2339), Alexa Fluor 647 mouse anti-human CD31 (clone WM59) was purchased from BD Pharmingen (561654), and

(7) PAR2 Activation on Human Kidney Tubular Epithelial Cells Induces Tissue Factor Synthesis, That Enhances Blood ClottingFrontiers in physiologyJanuary 1, 2021Iyer A, Humphries TLR, Owens EP, Zhao KN, Masci PP, Johnson DW, Nikolic-Paterson D, Gobe GC, Fairlie DP, Vesey DA.23119 was obtained from Cayman Chemicals (MI, United states) The human TF antibody (R&D Systems, Cat #AF2339) was used at 1/2000 dilution. The ALG-2-interacting protein X (ALIX) rabbit polyclonal antibody was

(8) Adipocytes express tissue factor and FVII and are procoagulant in a TF/FVIIa-dependent mannerUpsala journal of medical sciencesAugust 1, 2019Edén D, Panagiotou G, Mokhtari D, Eriksson JW, Åberg M, Siegbahn A.caspase-3 D175, pHSL s563, and HSL from Cell Signaling Technology, USA; TF N1C3 from GeneTex USA; TF AF2339 and TF AF3178 from R&D Systems, USA; IRDye 680CW and IRDye 800CW from LI-COR Biosciences, UK. Human

(9) The Low Molecular Weight Heparin Tinzaparin Attenuates Platelet Activation in Terms of Metastatic Niche Formation by Coagulation-Dependent and Independent PathwaysMolecules (Basel, Switzerland)October 24, 2018Gockel LM, Ponert JM, Schwarz S, Schlesinger M, Bendas G.sodium azide, DPBS). To label TF, the cells were incubated for 45 min with a primary anti-TF antibody (AF2339; R&D systems, Minneapolis, MN, USA) at a concentration of 2.5 µg/106 cells. Subsequently, the samples

(10) Sensitive and Specific Detection of Platelet-Derived and Tissue Factor-Positive Extracellular Vesicles in Plasma Using Solid-Phase Proximity Ligation AssayTH open : companion journal to thrombosis and haemostasisJuly 1, 2018Thulin Å, Yan J, Åberg M, Christersson C, Kamali-Moghaddam M, Siegbahn A.(557273) from BD Pharmingen (San Jose, California, United States). Goat anti-TF polyclonal antibody (AF2339) and goat IgG (AB-108-C) were from R&D Systems (Minneapolis, Minnesota, United States). Antibodies for flow cytometry

(11) Tissue factor is induced by interleukin-33 in human endothelial cells: a new link between coagulation and inflammationScientific reportsMay 4, 2016Stojkovic S, Kaun C, Basilio J, Rauscher S, Hell L, Krychtiuk KA, Bonstingl C, de Martin R, Gröger M, Ay C, et al.

(12) Fixed single-cell transcriptomic characterization of human radial glial diversityNature methodsJanuary 1, 2016Thomsen ER, Mich JK, Yao Z, Hodge RD, Doyle AM, Jang S, Shehata SI, Nelson AM, Shapovalova NV, Levi BP, et al.Signaling Technology; 60A8), Goat anti-human Coagulation Factor III/Tissue Factor (F3) (R&D Systems; AF2339), mouse anti-Ki67 (BD Biosciences, B56), rabbit anti-Ki67 (Abcam, ab15580), mouse anti-phospho-vimentin

(13) Interferon-γ and tumor necrosis factor-α act synergistically to up-regulate tissue factor in alveolar epithelial cellsExperimental lung researchOctober 1, 2011Bastarache JA, Sebag SC, Grove BS, Ware LB.milk in PBS), incubated for 2 h with blocking buffer and either goat-antihuman TF (R&D Systems, Cat. AF2339) at 0.1μg/ml, mouseantihuman TNFR1 (R&D Systems, Cat. MAB625) a 0.5μg/ml, or mouse-antihuman TNFR2 (R&D Systems

(14) Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cellsNature biotechnologyJuly 31, 2011Kelly OG, Chan MY, Martinson LA, Kadoya K, Ostertag TM, Ross KG, Richardson M, Carpenter MK, D'Amour KA, Kroon E, et al.

(15) Performance characteristics of combinations of host biomarkers to identify women with occult placental malaria: a case-control study from MalawiPloS oneJanuary 1, 2011Conroy AL, Liles WC, Molyneux ME, Rogerson SJ, Kain KC.

(16) Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel DiseaseCellJanuary 1, 0001"Kinchen J, Chen HH, Parikh K, Antanaviciute A, Jagielowicz M, Fawkner-Corbett D, Ashley N, Cubitt L, Mellado-Gomez E, Attar M, et al."COX-2,Bio-Techne,Cat#AF4198; RRID: AB_2229909 Goat polyclonal anti-human Coagulation Factor III/Tissue Factor,Bio-Techne,Cat#AF2339; RRID: AB_442150 Mouse monoclonal anti-human SOX6,Bio-Techne,"Cat#MAB7759, RRID: AB_2737259" Goat

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Human Coagulation Factor III/ Tissue Factor Antibody