Mouse/Rat Complement Component C3d Antibody AF2655 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse/Rat Complement Component C3d Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF2655
ApplicationsELISA Development,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Immunofluorescence,Immunocytochemistry,ELISA Capture
TargetComplement Component C3d
ReactivityHuman,Mouse,Rat,Transgenic Mouse
Conjugate/TagUnconjugated
IsotypeIgG
Quantity100 ug (also 25 ug)
HostGoat
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenE. coli-derived recombinant mouse Complement Component C3d, His1002-Arg1303 (Cys1010Ser), Accession # P01027
NCBI Full Gene Namecomplement component 3, complement C3
NCBI Gene AliasesCPAMD1, HEL-S-62p, ASP, ARMD9, Plp, HSE-MSF, C3a, C3b, AHUS5
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for detecting C3d in mouse retinal tissues?

Yes, the Complement Component C3d Antibody has been shown to work effectively in immunohistochemistry for detecting C3d in mouse retinal tissues, as demonstrated in studies analyzing retinal degeneration models ().

What is the recommended dilution for using this antibody in Western blot applications?

The antibody is recommended to be used at a dilution of 2 µg/ml for Western blot applications, as indicated in various studies that utilized this antibody for protein detection ().

Has this antibody been utilized in any studies related to neuroinflammation or neurodegeneration?

Yes, the Complement Component C3d Antibody has been referenced in studies investigating neuroinflammation and neurodegeneration, particularly in the context of retinal diseases and the complement system's role in these processes (, ).

Is this antibody suitable for use in ELISA assays?

Yes, the Complement Component C3d Antibody is suitable for use in ELISA assays, where it has been effectively employed to detect C3d levels in various experimental setups ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Knockout of Low‐Density Lipoprotein Receptor‐Related Protein 1 From Astrocytes in Adult Mice Accelerates Long‐Term Functional Recovery After Ischemic StrokeBrain and BehaviorApril 1, 2026Meng Wang, Sadiya N. Ahmad, Pamela Reed, Shane Sprague, Naomi L. Sayre18606). For determination of C3, two different antibodies were used. The first, goat anti‐C3d (Bio‐Techne AF2655) was used for initial studies in 24 h, 3 months, and 9 months post‐MCAO samples. Later experiments for

(2) The engineered probiotic strain Lactococcus lactis MG1363-pMG36e-GLP-1 regulates microglial polarization and gut dysbiosis in a transgenic mouse model of Parkinson’s diseaseNeural Regeneration ResearchMarch 1, 2026Mengyun Yue, Tingtao Chen, Wenjie Chen, Jing Wei, Bin Liao, Jie Zhang, Fangjun Li, Daojun Hong, Xin Fang,Western blotting GFAP,Mouse,1:10000,Proteintech,60190-1-lg,AB_2918946,Western blotting C3d,Goat,1:500,"R&D System, Minnesota, MN, USA",AF2655,AB_2066622,Western blotting β-Actin,Mouse,1:1000,CST,4970S,AB_3099713,Western blotting Anti-rabbit

(3) Regional Molecular Diversity in Chronic Spinal Cord InjuryCellular and Molecular NeurobiologyFebruary 21, 2026Chan-Il Choi, Ha Neui Kim, Hyesook Yoon, Maja Radulovic, Whitney L. Simon, Isobel A. Scarisbrick(S5545),AB_572263,1:4000 Arg1,Mouse,Santa Cruz (SC-271430),AB_10648473,1:200 C3D,Goat,R&D Systems (AF2655),AB_2066622,1:500 CC-1,Mouse,Millipore (OP80),AB_2057371,1:50 CD163,Mouse,Abcam (ab111250),AB_10888308,1:100 CD68,Rabbit,Abcam

(4) Cell-intrinsic vulnerability and immune activation cooperate to drive degeneration in a mitochondrial complex I deficiency model of optic neuropathyJournal of NeuroinflammationFebruary 6, 2026Daniela Santamaría-Muñoz, Raenier V. Reyes, Miranda R. Krueger, Andrea García-Llorca, Brennan Marsh-Armstrong, Xin Duan, Yang Hu, Derek S. Welsbie, Nicholas Marsh-Armstrong, Elisenda Sanz, et al.Antibody,Source,Catalog,Concentration Anti-C3d (goat),Biotechne,AF2655,1:50 Anti-Calretinin (goat),Swat,CG1,1:200 Anti-CCR2 (mouse),Biotechne,MAB150,1:50 Anti-CD3 FITC

(5) Mitochondrial Intercellular Transfer via Platelets After Physical Training Exerts Neuro‐Glial Protection Against Cerebral IschemiaMedComm (2020)February 1, 2026Toshiki Inaba, Nobukazu Miyamoto, Kenichiro Hira, Chikage Kijima, Yoshifumi Miyauchi, Hai‐Bin Xu, Kazo Kanazawa, Yuji Ueno, Nobutaka HattoriBD Biosciences; 558774), goat polyclonal anti‐C3d (inflammatory astrocyte marker; 1:50, R&D Systems; AF2655), rabbit polyclonal anti‐S100A10 (protective astrocyte marker; 1:100, Proteintech; 11250‐1‐AP), mouse

(6) Nicotinamide Attenuates Complement and Coagulation Pathways and Resultant Renal FibrosisFASEB JDecember 15, 2025Saori Kin, Yuji Oe, Taku Obara, Emiko Sato, Nobuyuki Takahashi, Mariko Miyazaki, Tetsuhiro Tanakatissue factor (1:200, #AF3178, R&D Systems, Minneapolis, MN), goat anti‐mouse C3d antibody (1:2000, #AF2655, R&D Systems, Minneapolis, MN, USA), rabbit anti‐human fibrin/fibrinogen antibody (1:4000, #A0080, Dako,

(7) The GLP1R Agonist Semaglutide Inhibits Reactive Astrocytes and Enhances the Efficacy of Neural Stem Cell Transplantation Therapy in Parkinson's Disease MiceAdvanced ScienceNovember 1, 2025Dan Song, Xiaoya Zou, Di Ma, Yuying Zhao, Tingting Liu, Bibiao Shen, Oumei Cheng(Proteintech, China, Cat#25859‐1‐AP), GFAP (Proteintech, China, Cat#16825‐1‐AP), C3d (R&D Systems, Cat#AF2655), NeuN (Abcam, UK, Cat#ab177487), Nestin (Abcam, UK, Cat#ab6142), TuJ1 (Abcam, UK, Cat#ab78078), and

(8) Intrathecal STAT3 inhibitor Bt354 ameliorates chronic constriction injury-induced nociceptive sensitization by modulating neuroinflammationNeurotherapeutics : the journal of the American Society for Experimental NeuroTherapeuticsOctober 1, 2025Cheng HJ, Chen NF, Tang YC, Shih PC, Chen WF, Chiu YJ, Sung CS, Wen ZH.monoclonal anti-CD86 antibody (Sc-28347, Santa Cruz, Dallas, Texas, USA), goat polyclonal anti-C3d (AF2655, R&D Systems, Inc., Minneapolis, MN, USA), rabbit monoclonal anti-LDHA (NBP2-67483, Novus Biologicals,

(9) Mesenchymal stromal cell secretome reduces lung injury and thrombo-inflammation induced by SARS-CoV-2 spike proteinStem Cell Research & TherapyJuly 1, 2025Anna Pezzotta, Alessandra Bovio, Barbara Imberti, Monica Locatelli, Daniela Corna, Domenico Cerullo, Sara Gastoldi, Ariela Benigni, Giuseppe Remuzzi, Marina Morigi, et al.anti-heparanase (1:150; ab85543, Abcam), goat anti-Factor H (1:100; A312, Quidel), goat anti-C3d (1:100; AF2655, R&D Systems), rat anti-GR1 antibody that recognizes the lymphocyte antigen 6 complex locus G6D (Ly6G;

(10) Luminal Vascular Dysfunction Drives Rapid Blood Brain Barrier Injury in Hyperglycemic Stroke: Key Roles for Luminal Glycocalyx and ComplementbioRxiv : the preprint server for biologyJune 28, 2025Chen H, Frank JA, Tan C, Lee AG, Kopchock R, Chiang T, Kim A, Galvan M, Fraser JF, Dornbos D, et al.

(11) Granzyme K activates the entire complement cascadeNatureMay 1, 2025Carlos A. Donado, Erin Theisen, Fan Zhang, Aparna Nathan, Madison L. Fairfield, Karishma Vijay Rupani, Dominique Jones, Kellsey P. Johannes, Accelerating Medicines Partnership RA/SLE Network, Jennifer Albrecht, et al.hour at room temperature followed by overnight incubation at 4 C with primary antibodies against C3d (AF2655, R&D Systems 1:100) and C4d (HP8033, HycultBiotech, 1:100). Secondary antibodies (Jackson ImmunoResearch)

(12) Moss-derived human complement factor H modulates retinal immune response and attenuates retinal degenerationJ NeuroinflammationApril 11, 2025Mandy Hector, Verena Behnke, Paulina Dabrowska-Schlepp, Andreas Busch, Andreas Schaaf, Thomas Langmann, Anne Wolfpowder in TBS‐T before incubation with primary antibody against C3d (1:2500 dilution, polyclonal goat, AF2655, R&D systems) or Actin (1:2500 dilution, monoclonal mouse, clone AC‐74, A2228, Sigma‐Aldrich). After

(13) Complement C3d enables cell-mediated immunity capable of distinguishing spontaneously transformed from nontransformed cellsProceedings of the National Academy of Sciences of the United States of AmericaDecember 24, 2024Platt JL, Zhao C, Chicca J, Pianko MJ, Han J, The S, Rao A, Keller ET, Garcia de Mattos Barbosa M, Naing L, et al.(0.01%). Slides were incubated overnight at 4 °C with goat IgG anti-mouse C3d antibody, (R&D Systems Cat: AF2655), diluted 1:125 in PBST containing 0.1% BSA. Slides were washed three times with PBST and incubated

(14) Analgesic Effect of Exercise on Neuropathic Pain via Regulating the Complement Component 3 of Reactive AstrocytesAnesthesia & AnalgesiaOctober 1, 2024Chenghao Wang, Hui He, Tianchi Gao, Xinzheng Sun, Lixia Du, Yayue Yang, Jianyu Zhu, Yachen Yang, Yanqing Wang, Wenli Mimouse antiGFAP (1:200, Cell Signaling Technology, 3670), goat antiComplement C3 (1:200, R&D Systems, AF2655), rabbit anti-Iba1 (1:400, Wako, 019-19741). After washing, free-floating sections were incubated for

(15) Complement C3d enables protective immunity capable of distinguishing spontaneously transformed from non-transformed cellsbioRxiv : the preprint server for biologyJuly 31, 2024Platt JL, Zhao C, Chicca J, Pianko MJ, Han J, The S, Rao A, Keller E, de Mattos Barbosa MG, Naing L, et al.

(16) Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasisBrain, behavior, and immunityJuly 1, 2024Jiao M, Wang C, Tang X, Dai C, Zhang N, Fan A, Qian Z, Liu S, Zhang F, Li B, et al.

(17) Gliosis-dependent expression of complement factor H truncated variants attenuates retinal neurodegeneration following ischemic injuryJournal of NeuroinflammationFebruary 22, 2024Josef Biber, Yassin Jabri, Sarah Glänzer, Aaron Dort, Patricia Hoffelner, Christoph Q. Schmidt, Oliver Bludau, Diana Pauly, Antje Groschenumber GFAP,Mouse,1:500,"Sigma Aldrich/Merck (Darmstadt, Germany)",G3893 C3d,Goat,2 µg/ml,"R&D Systems (Minneapolis, MN, USA)",AF2655 EGFP,Goat,1:200,"Rockland Immunochemicals (Limerick, PA, USA)",600 101 215 IBA-1,Rabbit,1:500,"Wako

(18) Effect of NLRP3 inflammasome induced astrocyte phenotype alteration in morphine toleranceFrontiers in pharmacologyJanuary 1, 2024Yuan Z, Lu B, Zhang M, Lu Y, Wang Z, Zhang W, Cheng H, Wu Z, Ji Q.overnight at 4°C with the following primary antibodies: Goat anti-C3d (1:2000; R&D Systems, United States, AF2655, RRID:AB_2066622), Rabbit anti-NLRP3 (1:2000; abcam, United States, ab263899, RRID:AB_2889890), Rabbit

(19) Mesenchymal Stem Cells Combined With Electroacupuncture Treatment Regulate the Subpopulation of Macrophages and Astrocytes to Facilitate Axonal Regeneration in Transected Spinal CordNeurospineDecember 31, 2023Rongyi Zhang, Junhua Wang, Qingwen Deng, Xingru Xiao, Xiang Zeng, Biqin Lai, Ge Li, Yuanhuan Ma, Jingwen Ruan, Inbo Han, et al.antichondroitin sulfate clone CS-56 (CSPGs, 1:100, Sigma Aldrich) for glial scars, goat anti-C3 (5 μg/mL; AF2655, R&D Systems, Minneapolis, MN, USA) for neurotoxic A1 reactive astrocytes, chicken anti-NF200 (1:1,000,

(20) Lethal adulthood myelin breakdown by oligodendrocyte-specific Ddx54 knockoutiScienceOctober 20, 2023Oizumi H, Miyamoto Y, Seiwa C, Yamamoto M, Yoshioka N, Iizuka S, Torii T, Ohbuchi K, Mizoguchi K, Yamauchi J, et al.monoclonal anti-S100A10,Abcam,Cat# ab76472; RRID: AB_1524359 goat polyclonal anti-C3d,R&D Systems,Cat# AF2655; RRID: AB_2066622 rabbit polyclonal anti-iNOS,"Novus Biochemicals, LLC",Cat# NBP1-50606; RRID: AB_10012794 rabbit

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Complement Component C3d Antibody

Complement Component C3d Antibody

Complement Component C3d Antibody

Complement Component C3d Antibody

Complement Component C3d Antibody

Mouse/Rat Complement Component C3d Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (74)

Reviews

Mouse/Rat Complement Component C3d Antibody from R&D Systems, a Bio-Techne Brand

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