SLPI Antibody AF1274 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemSLPI Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF1274
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Mass Spectrometry,Neutralization,Chromatin Immunoprecipitation (ChIP),Immunocytochemistry,ELISA Detection
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ReactivityBacteria - Borrelia burgdorferi,Bacteria - Stenotrophomonas maltophilia,Human,Rat,Bacteria - Borrelia burgdorferi (Lyme disease spirochete)
HostGoat
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
IsotypeIgG
TargetSLPI
ClonalityPolyclonal
Antibody TypePrimary Antibodies
NCBI Gene AliasesALK1, ALP, BLPI, HUSI, HUSI-I, MPI, WAP4, WFDC4
NCBI Full Gene Namesecretory leukocyte peptidase inhibitor
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
PurityAntigen Affinity-purified
ConcentrationLYOPH
ImmunogenE. coli-derived recombinant human SLPI

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human SLPI in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with mouse SLPI is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Autologous cell therapy with CD133+ bone marrow-derived stem cells for Asherman Syndrome: a phase 1/2 trialNature communicationsJanuary 2, 2026Santamaria X, Pardo-Figuerez M, González-Fernández J, Querol S, Rodríguez L, Valcárcel D, González C, Fernandez E, Amorós D, Robles M, et al.with goat anti-ANGPTL2 primary antibody (R&D Systems, Cat# AF623) or goat anti-SLPI (R&D Systems Cat #AF1274), both diluted 1:100 in blocking buffer. After washing with PBS, sections were incubated for 1 h at

(2) Secretory leukocyte protease inhibitor influences periarticular joint inflammation in Borrelia burgdorferi-infected miceElifeMay 20, 2025Qian Yu, Xiaotian Tang, Thomas Hart, Robert Homer, Alexia A Belperron, Linda K Bockenstedt, Aaron Ring, Akira Nakamura, Erol Fikrigabove) at 4°C for 2 h. The binding was detected with goat anti-human or murine SLPI (R&D Systems, #AF1274 and AF1735) and Alexa Fluor 488 or Alexa Fluor 647 donkey anti-goat IgG (H+L) (Invitrogen, #A32814,

(3) Secretory leukocyte protease inhibitor influences periarticular joint inflammation in Borrelia burgdorferi-infected miceElifeMay 20, 2025Qian Yu, Xiaotian Tang, Thomas Hart, Robert Homer, Alexia A Belperron, Linda K Bockenstedt, Aaron Ring, Akira Nakamura, Erol Fikrigabove) at 4°C for 2 h. The binding was detected with goat anti-human or murine SLPI (R&D Systems, #AF1274 and AF1735) and Alexa Fluor 488 or Alexa Fluor 647 donkey anti-goat IgG (H+L) (Invitrogen, #A32814,

(4) Secretory leukocyte protease inhibitor influences periarticular joint inflammation in B. burgdorferi-infected micebioRxiv : the preprint server for biologyMarch 28, 2025Yu Q, Tang X, Hart T, Homer R, Belperron AA, Bockenstedt LK, Ring A, Nakamura A, Fikrig E.

(5) Protease-anti-protease compartmentalization in SARS-CoV-2 ARDS: Therapeutic implicationsEBioMedicineMarch 1, 2022McElvaney OF, Asakura T, Meinig SL, Torres-Castillo JL, Hagan RS, Gabillard-Lefort C, Murphy MP, Thorne LB, Borczuk A, Reeves EP, et al.AAT (1:2000, GR286990-3, Abcam), NE (1:1000, clone # 950317, MAB91671, R&D systems) and SLPI (1:2000, AF1274, R&D systems, RRID: AB_2302508), were incubated overnight at 4 °C with the target protein, then probed

(6) Neutrophil elastase selectively kills cancer cells and attenuates tumorigenesisCellJune 10, 2021Cui C, Chakraborty K, Tang XA, Zhou G, Schoenfelt KQ, Becker KM, Hoffman A, Chang YF, Blank A, Reardon CA, et al.anti-SERPINB1,Mybiosource,Cat# 128870 Goat anti-SLPI,R&D systems,Cat# AF1735 Goat anti-SLPI,R&D systems,Cat# AF1274 "Rat anti-CD45, APC efluor 780",ThermoFisher Scientific,Cat# 47-0451 "Rat anti-CD11b, PE-Cy7",ThermoFisher

(7) SLPI is a critical mediator that controls PTH-induced bone formationNature communicationsApril 9, 2021Morimoto A, Kikuta J, Nishikawa K, Sudo T, Uenaka M, Furuya M, Hasegawa T, Hashimoto K, Tsukazaki H, Seno S, et al.to mature osteoclasts. To assess the effect of SLPI antibody on cell-cell contact, SLPI antibodies (AF1274, R&D Systems; S0635, US Biological Life Sciences, Salem, MA, USA) 12.5 µg/mL, or control goat IgG (Santa

(8) Characterisation of the Major Extracellular Proteases of Stenotrophomonas maltophilia and Their Effects on Pulmonary AntiproteasesPathogens (Basel, Switzerland)June 28, 2019Molloy K, Smith SG, Cagney G, Dillon ET, Greene CM, McElvaney NG.

(9) Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversityNature communicationsMay 23, 2018Nguyen QH, Pervolarakis N, Blake K, Ma D, Davis RT, James N, Phung AT, Willey E, Kumar R, Jabart E, et al.

(10) Secretory leukocyte peptidase inhibitor expression and apoptosis effect in oral leukoplakia and oral squamous cell carcinomaOncology reportsApril 1, 2018Wang X, Jin Y, Li YX, Yang Y.Billerica, MA, USA) and incubated with a goat polyclonal anti-SLPI antibody (dilution 0.1 µg/ml; cat. no. AF1274; R&D Systems, Minneapolis, MN, USA). The loading control was the mouse monoclonal GAPDH antibody (dilution

(11) Neutrophils and Activated Macrophages Control Mucosal Immunity by Proteolytic Cleavage of AntileukoproteinaseFrontiers in immunologyJanuary 1, 2018Vandooren J, Goeminne P, Boon L, Ugarte-Berzal E, Rybakin V, Proost P, Abu El-Asrar AM, Opdenakker G.0.1% Tween 20, 50 mM Tris, pH 7.5). Next, the membranes were incubated overnight with goat anti-hSLPI (AF1274, R&D Systems, Minneapolis, MN, USA), mouse anti-hNE (MAB91671, R&D Systems, Minneapolis, MN, USA), or

(12) Secretory leukocyte protease inhibitor reverses inhibition by CNS myelin, promotes regeneration in the optic nerve, and suppresses expression of the transforming growth factor-β signaling protein Smad2The Journal of neuroscience : the official journal of the Society for NeuroscienceMarch 20, 2013Hannila SS, Siddiq MM, Carmel JB, Hou J, Chaudhry N, Bradley PM, Hilaire M, Richman EL, Hart RP, Filbin MT.

(13) Secretory leukocyte protease inhibitor (SLPI) expression and tumor invasion in oral squamous cell carcinomaThe American journal of pathologyJune 1, 2011Wen J, Nikitakis NG, Chaisuparat R, Greenwell-Wild T, Gliozzi M, Jin W, Adli A, Moutsopoulos N, Wu T, Warburton G, et al.

(14) Osteochondral angiogenesis and increased protease inhibitor expression in OAOsteoarthritis and cartilageApril 1, 2010Fransès RE, McWilliams DF, Mapp PI, Walsh DA.Nottingham, UK. Monoclonal anti-PAI-1 (3785) was from Axis-Shield UK, Kimbolton, UK. Polyclonal anti-SLPI (AF1274) was from R&D Systems, Abingdon, UK. Polyclonal anti-VEGF (A20) was from Insight Biotechnology, London,

(15) Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patientsMolecular & cellular proteomics : MCPOctober 1, 2008Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, et al.

(16) The major bactericidal activity of human seminal plasma is zinc-dependent and derived from fragmentation of the semenogelinsJournal of immunology (Baltimore, Md. : 1950)September 1, 2008Edström AM, Malm J, Frohm B, Martellini JA, Giwercman A, Mörgelin M, Cole AM, Sørensen OE.

(17) The secretory leukocyte protease inhibitor (SLPI) and the secondary granule protein lactoferrin are synthesized in myelocytes, colocalize in subcellular fractions of neutrophils, and are coreleased by activated neutrophilsJournal of leukocyte biologyMay 1, 2008Jacobsen LC, Sørensen OE, Cowland JB, Borregaard N, Theilgaard-Mönch K.

(18) The alarm anti-protease, secretory leukocyte protease inhibitor, is a proliferation and survival factor for ovarian cancer cellsCarcinogenesisMarch 1, 2008Simpkins FA, Devoogdt NM, Rasool N, Tchabo NE, Alejandro EU, Kamrava MM, Kohn EC.

(19) cDNA microarray analysis of invasive and tumorigenic phenotypes in a breast cancer modelLaboratory investigation; a journal of technical methods and pathologyMarch 1, 2004Kluger HM, Kluger Y, Gilmore-Hebert M, DiVito K, Chang JT, Rodov S, Mironenko O, Kacinski BM, Perkins AS, Sapi E.

(20) Antimicrobial components of vaginal fluidAmerican journal of obstetrics and gynecologySeptember 1, 2002Valore EV, Park CH, Igreti SL, Ganz T.

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SLPI Antibody

SLPI Antibody

SLPI Antibody

SLPI Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (21)

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SLPI Antibody from R&D Systems, a Bio-Techne Brand

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