CCL5/RANTES Antibody AF-278-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCCL5/RANTES Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF-278-NA
NCBI Gene AliasesSIS-delta, D17S136E, SISd, TCP228, RANTES, SCYA5, eoCP
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetCCL5/RANTES
HostGoat
Quantity100 ug (also 25 ug)
IsotypeIgG
ApplicationsWB, FCM, ICC, IHC, IHC-fr, IHC-p, IP, FA, Neut
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human CCL5/RANTES, Ser24-Ser91, Accession # P13501
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
ReactivityHu, Ms, RhMk
NCBI Full Gene NameC-C motif chemokine ligand 5

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human CCL5/RANTES in direct ELISAs. Detects human CCL5/RANTES and mouse CCL5/RANTES Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant canine CCL5/RANTES is observed and approximately 20% cross-reactivity with recombinant cotton rat CCL5/RANTES is observed. In Western blots, no cross-reactivity with recombinant human (rh) CCL3 and rhCCL4 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) RhoB supports rubella infection and impairs endothelial barrier integrity through downstream ROCK signalingCell Commun SignalApril 27, 2026Igor Kovacevic, Marie Müller, Leonie Mair, Vivien Henschke, Annkatrin Kowalski, Jes-Niels Boeckel, Karolin Kropf, Guido Posern, Uta Reibetanz, Claudia Clausimmunofluorescence analysis at a 1:100 dilution. The neutralizing polyclonal goat IgG antibody (Cat#AF-278-NA, Bio-Techne) targeting human CCL5/RANTES was used at 0.1 µg/ml and applied 8 and 20 h after RuV infection.

(2) PP4 modulates macrophage-neutrophil crosstalk to restrict CCL5 -driven NETosis in sepsisRedox BiologyApril 1, 2026Feng-Ming Yang, Shih-Chang Hsu, Yu-Chih Wu, Ching-Lu Chen, Tse-Hua Tan, Szu-Yi Chou, Hui-Ming Chang, Edward T.H. Yeh(Taipei, Taiwan) and were prepared as previously described [35]. A neutralizing antibody for CCL5 (#AF-278-NA) was obtained from R&D Systems (Minneapolis, MN, USA). 2.5 Generation of conditioned medium (CM) Primary

(3) C-C chemokine ligand 5 from women subcutaneous adipose tissue has a central role in vascular agingCardiovasc DiabetolJuly 18, 2025Laura Le Pelletier, Kenza Ngono Ayissi, Jennifer Gorwood, Emilie Capel, Romain Morichon, Matthieu Mantecon, Martine Auclair, Rohia Alili, Christine Katlama, Lise Cuzin, et al.of CCL5 in endothelial cell dysfunction, a CCL5 neutralizing antibody or control isotype antibody (#AF-278-NA, RRID: AB_354440 or #AB-108-C, RRID: AB_354267 respectively, Biotechne, Minneapolis, MN, USA, 0.1 µg/mL)

(4) Low CCR5 expression protects HIV-specific CD4+ T cells of elite controllers from viral entryNature communicationsJanuary 26, 2022Claireaux M, Robinot R, Kervevan J, Patgaonkar M, Staropoli I, Brelot A, Nouël A, Gellenoncourt S, Tang X, Héry M, et al.blocking antibodies (polyclonal antibodies from goat, R&D Systems) including anti-CCL5 (1:200 dilution, #AF-278-NA), anti-CCL4 (1:67 dilution, #AF-271-NA), and anti-CCL3 (1:20 dilution, #AF-270-NA), using a concentration

(5) Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial CellsmBioAugust 31, 2021Mladinich MC, Conde JN, Schutt WR, Sohn SY, Mackow ER.(catalog number G9545) was obtained from Sigma-Aldrich. Neutralizing antibodies to CCL5 (catalog number AF-278-NA), CCR3 (catalog number MAB155), CCR5 (catalog number MAB182), and CCL5/RANTES DuoSet ELISA kits were

(6) The Protein Kinase Receptor Modulates the Innate Immune Response against Tacaribe VirusVirusesJuly 7, 2021Moreno H, Kunz S.

(7) Dissecting spatial heterogeneity and the immune-evasion mechanism of CTCs by single-cell RNA-seq in hepatocellular carcinomaNature communicationsJuly 2, 2021Sun YF, Wu L, Liu SP, Jiang MM, Hu B, Zhou KQ, Guo W, Xu Y, Zhong Y, Zhou XR, et al.incubated for 30 min with primary antibody FoxP3 (Cat# ab20034, diluted 1:250, Abcam) and CCL5 (Cat# AF-278-NA, diluted 1:250, R&D Systems). The EnVision G/2 Double stain system (DAKO) was used for dual-color antigen

(8) PARP inhibitors promote stromal fibroblast activation by enhancing CCL5 autocrine signaling in ovarian cancerNPJ precision oncologyJune 9, 2021Li X, Fang T, Xu S, Jin P, Zhou D, Wang Z, Li H, Yang Z, Chen G, Zheng X, et al.Transwell apparatus, and 600 μl of CM collected from Ola or Ola with anti-CCL5 (Human CCL5 antibody, AF-278-NA, R&D Systems, USA), or dimethyl sulfoxide (DMSO)-treated MRC5-CAFs or primary CAFs, was added into the

(9) Preterm Birth Is Associated With Immune Dysregulation Which Persists in Infants Exposed to Histologic ChorioamnionitisFrontiers in immunologyJanuary 1, 2021Sullivan G, Galdi P, Borbye-Lorenzen N, Stoye DQ, Lamb GJ, Evans MJ, Skogstrand K, Chandran S, Boardman JP.Sino Biological, Eschborn, Germany), C9 (R-plex kit #F21XZ, MSD), MMP-9 (BAF911 and MAB911), RANTES (MAB278 and AF278NA) and CRP (BAM17072 and MAB1701) (all R&D Systems, Minneapolis, US) for coating the U-plex plate and

(10) Titanium salts tested in reconstructed human skin with integrated MUTZ-3-derived Langerhans cells show an irritant rather than a sensitizing potentialContact dermatitisNovember 1, 2020Rodrigues Neves CT, Spiekstra SW, de Graaf NPJ, Rustemeyer T, Feilzer AJ, Kleverlaan CJ, Gibbs S.experiments, previously established optimal blocking concentrations of 7 μg/mL goat antihuman CCL5 (AF‐278‐NA; R&D systems, Minneapolis, Minnesota), 7 μg/mL goat antihuman CXCL12 (AF‐310‐NA, R&D systems), or 7

(11) Controlled release of biological factors for endogenous progenitor cell migration and intervertebral disc extracellular matrix remodellingBiomaterialsSeptember 1, 2020Frapin L, Clouet J, Chédeville C, Moraru C, Samarut E, Henry N, André M, Bord E, Halgand B, Lesoeur J, et al.temperature. After three wash steps with PBS, the PMBs were incubated overnight at 4 °C with human CCL5 (# AF-278-NA, R&D Systems Inc., Minneapolis, MN, USA) at 10 µg/mL in PBS1X. Samples were then incubated for 1 h at

(12) CCR5/CCL5 axis interaction promotes migratory and invasiveness of pancreatic cancer cellsScientific reportsJanuary 22, 2018Singh SK, Mishra MK, Eltoum IA, Bae S, Lillard JW Jr, Singh R.

(13) Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loopOncotargetDecember 15, 2017Lin S, Sun L, Lyu X, Ai X, Du D, Su N, Li H, Zhang L, Yu J, Yuan S.(#70109, 1:1200), Jagged 2 (#2205, 1:1000), Notch 1 (#3608, 1:1000), Notch 2 (#5732, 1:1000). CCL5 (AF-278-NA, 15μg/ml), CCR5 (MAB1802, 1μg/ml; MAB181, 25μg/ml), TGF-β1 (MAB240, 20μg/ml) were purchased from R&D Systems

(14) An initial investigation into endothelial CC chemokine expression in the human rheumatoid synoviumCytokineSeptember 1, 2017Rump L, Mattey DL, Kehoe O, Middleton J.

(15) The Ovarian Cancer Chemokine Landscape Is Conducive to Homing of Vaccine-Primed and CD3/CD28-Costimulated T Cells Prepared for Adoptive TherapyClinical cancer research : an official journal of the American Association for Cancer ResearchJune 15, 2015Zsiros E, Duttagupta P, Dangaj D, Li H, Frank R, Garrabrant T, Hagemann IS, Levine BL, June CH, Zhang L, et al.with the following antibody clones: CCL2 (HPA019163, Sigma-Aldrich), CCL4 (1738-1, Epitomics), CCL5 (AF-278-NA, R&D Systems), CCL28 (MAB7171, R&D Systems), CXCL10 (ab9807, Abcam), CXCL12 (SC-28876, Santa Cruz),

(16) NK cell responses to simian immunodeficiency virus vaginal exposure in naive and vaccinated rhesus macaquesJournal of immunology (Baltimore, Md. : 1950)July 1, 2014Shang L, Smith AJ, Duan L, Perkey KE, Qu L, Wietgrefe S, Zupancic M, Southern PJ, Masek-Hammerman K, Reeves RK, et al.

(17) Lenalidomide inhibits lymphangiogenesis in preclinical models of mantle cell lymphomaCancer researchDecember 15, 2013Song K, Herzog BH, Sheng M, Fu J, McDaniel JM, Chen H, Ruan J, Xia L.

(18) Correction: Human Bone Marrow Mesenchymal Stem Cells Induce Collagen Production and Tongue Cancer InvasionPLoS ONENovember 15, 2013Sirpa Salo et al.30 min. Slides were incubated overnight at 4°C with the polyclonal goat anti-human CCL5 antibody (#AF-278NA, R&D Systems) at a 1:100 dilution. For the polyclonal rabbit anti-human antibody PINP [31] slides were

(19) Human bone marrow mesenchymal stem cells induce collagen production and tongue cancer invasionPloS oneJanuary 1, 2013Salo S, Bitu C, Merkku K, Nyberg P, Bello IO, Vuoristo J, Sutinen M, Vähänikkilä H, Costea DE, Kauppila JH, et al.30 min. Slides were incubated overnight at 4°C with the polyclonal goat anti-human CCL5 antibody (#AF-278-NA, R&D Systems) at a 1:100 dilution. For the polyclonal rabbit anti-human antibody PINP [31] slides were

(20) Expression and regulation of chemokines in murine and human type 1 diabetesDiabetesFebruary 1, 2012Sarkar SA, Lee CE, Victorino F, Nguyen TT, Walters JA, Burrack A, Eberlein J, Hildemann SK, Homann D.approved the utility of the following human chemokine–specific antibodies: goat polyclonals αCCL5 (AF278-NA), αCCL8 (AF281-NA), αCXCL9 (AF392), αCXCL10 (AF266-NA), and αCX3CL1 (AF365) as well as polyclonal chicken

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CCL5/RANTES Antibody

CCL5/RANTES Antibody

CCL5/RANTES Antibody

CCL5/RANTES Antibody

CCL5/RANTES Antibody

CCL5/RANTES Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (37)

Reviews

CCL5/RANTES Antibody from R&D Systems, a Bio-Techne Brand

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