GDNF Antibody AF-212-NA from R&D Systems, a Bio-Techne Brand

Product Specs
ItemGDNF Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF-212-NA
NCBI Gene AliasesATF, HFB1-GDNF, ATF1, HSCR3, ATF2
ApplicationsAffinity Assay,ELISA Development (Detection),Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Neutralization,Immunocytochemistry
TargetGDNF
ReactivityAvian - Chicken,Human,Mouse,Primate - Callithrix jacchus (Common Marmoset),Primate - Macaca mulatta (Rhesus Macaque),Rat,Transgenic Mouse
Quantity100 ug (also 25 ug)
HostGoat
Conjugate/TagUnconjugated
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
NCBI Full Gene Nameglial cell derived neurotrophic factor
ClonalityPolyclonal
ImmunogenE. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF, Arg109-Ile211, Accession # P39905
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and rat GDNF in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Neurons enhance blood–brain barrier function via upregulating claudin-5 and VE-cadherin expression due to glial cell line-derived neurotrophic factor secretionElifeOctober 30, 2024Lu Yang, Zijin Lin, Ruijing Mu, Wenhan Wu, Hao Zhi, Xiaodong Liu, Hanyu Yang, Li Liuanti-GDNF antibody Exogenous and endogenous GDNF in the medium was neutralized with anti-GDNF antibody (#AF-212-NA, R&D system, Minneapolis, MN, USA). 0.25, 0.5, and 1.0 μg/ml anti-GDNF antibody was added into the US-CM

(2) Neurons enhance blood–brain barrier function via upregulating claudin-5 and VE-cadherin expression due to glial cell line-derived neurotrophic factor secretioneLifeOctober 30, 2024Lu Yang, Zijin Lin, Ruijing Mu, Wenhan Wu, Hao Zhi, Xiaodong Liu, Hanyu Yang, Li Liuanti-GDNF antibody Exogenous and endogenous GDNF in the medium was neutralized with anti-GDNF antibody (#AF-212-NA, R&D system, Minneapolis, MN, USA). 0.25, 0.5, and 1.0 μg/ml anti-GDNF antibody was added into the US-CM

(3) GDNF gene therapy for alcohol use disorder in male non-human primatesNature medicineAugust 1, 2023Ford MM, George BE, Van Laar VS, Holleran KM, Naidoo J, Hadaczek P, Vanderhooft LE, Peck EG, Dawes MH, Ohno K, et al.MAB318), 1:1,000 monoclonal rabbit anti-GFP (Molecular Probes, G10362), or goat-anti-GDNF (R&D Systems, AF-212-NA). Probed tissue was then incubated for 1 h with horse anti-mouse HRP (Vector, MP-7402) or goat-on-rodent HRP Polymer

(4) Intrathecal Injection of Mesenchymal Stromal Cell Cultured on 3D Fiber Ameliorates Multiple Organ Damage in Murine LupusStem cells translational medicineJune 22, 2022Saito Y, Miyajima M, Yamamoto S, Miura N, Sato T, Kita A, Ijima S, Fujimiya M, Chikenji TS.without recombinant GDNF protein (50 ng/mL; 700401; Biolegend) and GDNF-neutralizing antibody (1 µg/mL; AF-212-NA; R&D Systems, Minneapolis, MN, USA) for 48 hours. To prepare conditioned media (CM) from 2D-BMSC and

(5) Exploring the potential application of dental pulp stem cells in neuroregenerative medicineNeural regeneration researchApril 1, 2022Sultan N, Scheven BA.presence of specific neutralising antibodies. Antibodies against GDNF (1 µg/mL; R&D systems, Oxon, UK; AF-212-NA) and NT-3 (2 µg/mL; R&D systems; Cat# AF1404) were used to test the impact of their absence on TG neuronal

(6) Dental pulp stem cells stimulate neuronal differentiation of PC12 cellsNeural regeneration researchSeptember 1, 2021Sultan N, Amin LE, Zaher AR, Grawish ME, Scheven BA.

(7) TGF-β1 Increases GDNF Production by Upregulating the Expression of GDNF and Furin in Human Granulosa-Lutein CellsCellsJanuary 10, 2020Yin J, Chang HM, Yi Y, Yao Y, Leung PCK.was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). A goat anti-human GDNF antibody (AF-212-NA; diluted at 1:1000; RRID: AB_2111398) was obtained from R&D Systems (Minneapolis, MN). Polyclonal rabbit

(8) Spatial and temporal immunoreactivity in the rat brain using an affinity purified polyclonal antibody to DNSP-11Journal of chemical neuroanatomyOctober 1, 2019Sonne JWH, Groshong JS, Seavey C, Gash DM.Chemicon (MAB318, Millipore Chemicon, Temecula, CA). GDNF antibody was purchased from R&D Systems (AF-212-NA, R&D Systems, Minneapolis, MN). Secondary antibodies include peroxidase-conjugated anti-rabbit IgG (PI-1000,

(9) Widespread Striatal Delivery of GDNF from Encapsulated Cells Prevents the Anatomical and Functional Consequences of ExcitotoxicityNeural plasticityJanuary 1, 2019Emerich DF, Kordower JH, Chu Y, Thanos C, Bintz B, Paolone G, Wahlberg LU.bovine serum albumin or 5% normal horse serum. Tissue sections were immunostained for GDNF (R&D Systems, AF-212-NA; 1 : 500) and NeuN (Millipore, MAB377; 1 : 1000) overnight at room temperature. After 6 washes, the

(10) Enhancement of HGF-induced tubulogenesis by endothelial cell-derived GDNFPloS oneJanuary 1, 2019Nakasatomi M, Takahashi S, Sakairi T, Ikeuchi H, Kaneko Y, Hiromura K, Nojima Y, Maeshima A.Koken (Tokyo, Japan). Antibodies used in this study were as follows: goat polyclonal anti-GDNF antibody (AF-212-NA) (R&D Systems); mouse anti-beta-actin antibody (3598R-100) (BioVision, Milpitas, CA); rabbit monoclonal

(11) Destabilizing Domains Enable Long-Term and Inert Regulation of GDNF Expression in the BrainMolecular therapy. Methods & clinical developmentDecember 14, 2018Quintino L, Namislo A, Davidsson M, Breger LS, Kavanagh P, Avallone M, Elgstrand-Wettergren E, Isaksson C, Lundberg C.rabbit anti-pRPS6 (#2211, 1:400, Cell Signaling Technology, Danvers, MA, USA), goat anti-human GDNF (AF-212-NA, 1:1,000, R&D Systems Europe), rabbit anti-DHFR DD (1:20,000, a kind gift from the Wandless lab that

(12) Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodiesProceedings of the National Academy of Sciences of the United States of AmericaJanuary 30, 2018Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ.

(13) Pre-α-pro-GDNF and Pre-β-pro-GDNF Isoforms Are Neuroprotective in the 6-hydroxydopamine Rat Model of Parkinson's DiseaseFrontiers in neurologyJanuary 1, 2018Penttinen AM, Parkkinen I, Voutilainen MH, Koskela M, Bäck S, Their A, Richie CT, Domanskyi A, Harvey BK, Tuominen RK, et al.(DAT),Rat,Millipore,MAB369,AB_2190413,"1:2,000" Glial cell line-derived neurotrophic factor (GDNF),Goat,R & D systems,AF-212-NA,AB_2111398,"1:3,000a 1:1,000b" Green fluorescent protein (GFP),Rabbit,Life technologies,A11122,N/A,"1:2,000" Tyrosine

(14) Neurturin is a PGC-1α1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formationMolecular metabolismJanuary 1, 2018Mills R, Taylor-Weiner H, Correia JC, Agudelo LZ, Allodi I, Kolonelou C, Martinez-Redondo V, Ferreira DMS, Nichterwitz S, Comley LH, et al.curve of known antigen (Figure S4). Antibody-related information for Proseek probes: GDNF (R&D Systems AF-212-NA), IL-15 (R&D Systems AF-447), NRTN (R&D Systems AF-477), NGF (R&D Systems AF-256-NA). Antigen related

(15) Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injuryScientific reportsOctober 18, 2017Nagashima K, Miwa T, Soumiya H, Ushiro D, Takeda-Kawaguchi T, Tamaoki N, Ishiguro S, Sato Y, Miyamoto K, Ohno T, et al.

(16) The noble gas xenon provides protection and trophic stimulation to midbrain dopamine neuronsJournal of neurochemistryJuly 1, 2017Lavaur J, Le Nogue D, Lemaire M, Pype J, Farjot G, Hirsch EC, Michel PP.(GDNF) was from Eurobio AbCys (Courtaboeuf, France). The goat anti‐human/rat neutralizing GDNF antibody (AF‐212‐NA; RRID:AB_2111398) was purchased from R&D Systems Europe (Lille, France). Cell cultures protocols Animals

(17) Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1^G93A ALS rat but has adverse side effectsGene therapyApril 1, 2017Thomsen GM, Alkaslasi M, Vit JP, Lawless G, Godoy M, Gowing G, Shelest O, Svendsen CN.onto pre-coated slides at 25 μm. Tissue was stained using anti-GDNF (1:100, puriﬁed goat IgG, Cat # AF-212-NA, R&D Systems, Minneapolis, MN, USA). Injections At ~ p25, rats were administered tail vein injections

(18) Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal MembraneScientific reportsMarch 9, 2017Dong Y, Kanda A, Noda K, Saito W, Ishida S.

(19) CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's diseasePloS oneJanuary 1, 2017Stott SR, Hayat S, Carnwath T, Garas S, Sleeman JP, Barker RA.sc-19651, 1:50; Santa Cruz), rat-dopamine transporter (DAT; 1:200; MAB369, Chemicon), Goat-GDNF (1:500; AF-212-NA; R&D systems), and Rabbit-TH (1:1000, Pelfreez). A one-hour incubation with an appropriate biotinylated

(20) Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repairThe Journal of biological chemistryMay 23, 2014Wang TY, Bruggeman KA, Sheean RK, Turner BJ, Nisbet DR, Parish CL.

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GDNF Antibody

GDNF Antibody

GDNF Antibody

GDNF Antibody

GDNF Antibody

GDNF Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

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GDNF Antibody from R&D Systems, a Bio-Techne Brand

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