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Antibodies
Antibodies
Anti-CD3 antibody [SP7]
Anti-CD3 antibody [SP7] from Abcam
(2)
Write a review
Citations:
(614)
Supplier Page
Supplier Page from
Abcam for
Anti-CD3 antibody [SP7]
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-CD3 antibody [SP7]
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab16669
Antibody Type
Primary
Quantity
1 ML, 100 UL, 500 UL
Host
Rabbit
Clonality
Monoclonal
Clone
SP7
Applications
Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot, Flow Cytometry, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Multiplex immunohistochemistry, Western blot
Isotype
IgG
Conjugate/Tag
Unconjugated
Research Area
Immuno-oncology
Gene Name
CD3E
Reactivity
Human, Mouse, Rat
Target
CD3
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (614)
(1)
Sex Differences in Left-Ventricular Strain in a Murine Model of Coxsackievirus B3 Myocarditis
iScience
December 15, 2023
Damian N. Di Florio, Logan P. Macomb, Presley G. Giresi, Danielle J. Beetler, Natalie E. Bonvie-Hill, Katie A. Shapiro, Abdel-Rahman N. Naser, Sami Khatib, Emily R. Whelan, Gabriel J. Weigel,
et al.
103102
,
1:200
,
rat
),
CD11b
(Abcam, Cambridge, United Kingdom,
ab133357
,
1:3000
, rabbit), CD3 (
Abcam
,
ab16669
,
1:200
, rabbit), and
F4/80
(
BioRad
, Hercules, CA,
MCA497G
,
1:250
,
rat
). A Envision+ anti-rabbit labeled
(2)
Components of the tumor immune microenvironment based on m-IHC correlate with prognosis and subtype of triple-negative breast cancer
Cancer Med
December 7, 2023
Luyi Lin, Haiming Li, Xin Wang, Zezhou Wang, Guanhua Su, Jiayin Zhou, Shiyun Sun, Xiaowen Ma, Yan Chen, Chao You,
et al.
[C‐11] antibodies to pancytokeratin (
ab7753
,
Abcam
,
1:150
), rabbit monoclonal [SP7] antibodies to CD3 (
ab16669
,
Abcam
,
1:150
), rabbit monoclonal [EPR22102‐37] antibodies to
FoxP3
(
ab215206
,
Abcam
,
1:150
), and DAPI.
(3)
Heterogeneity in tertiary lymphoid structures predicts distinct prognosis and immune microenvironment characterizations of clear cell renal cell carcinoma
J Immunother Cancer
December 1, 2023
Wenhao Xu, Jiahe Lu, Wang-Rui Liu, Aihetaimujiang Anwaier, Yuhao Wu, Xi Tian, Jia-Qi Su, Yuan-Yuan Qu, Jianfeng Yang, Hailiang Zhang,
et al.
histological features resembling
lymphoid tissues
with B cells (CD20,
ab64088
,
Abcam
, USA),
T cells
(CD3,
ab16669
,
Abcam
), follicular DCs (FDCs) (CD21,
ab7290
,
Abcam
), and
germinal center
(GC) cells (CD23,
ab16702
,
(4)
Harnessing IGF-1 and IL-2 as biomarkers for calcineurin activity to tailor optimal FK506 dosage in α-synucleinopathies
Frontiers in Molecular Biosciences
November 29, 2023
Sofia Zaichick, Gabriela Caraveo
primary antibodies were used: MAP2 (
Millipore
,
AB5622
), Iba1 (Waco, 019-19741,
1:250
), CD3 (
Abcam
,
ab16669
,
1:250
), and CD24 (
Santa Cruz
, #
sc-11406
,
1:100
). The secondary antibodies used were Alexa 488 (
Invitrogen
,
(5)
Cooperation of MLL1 and Jun in controlling H3K4me3 on enhancers in colorectal cancer
Genome Biology
November 27, 2023
Xiang Lin, Ji-Dong Chen, Chen-Yu Wang, Zhen Cai, Rui Zhan, Chen Yang, La-Ying Zhang, Lian-Yun Li, Yong Xiao, Ming-Kai Chen,
et al.
RRID: AB_2118291), MLL1 (CST, 14,197, RRID:AB_2688010), JUN (
CST
,
9165
, RRID:AB_2130165), CD3 (
Abcam
,
ab16669
, RRID:AB_443425), CD4 (
Abcam
,
ab183685
, RRID:AB_2686917),
CD8
(
Abcam
,
ab217344
, RRID:AB_2890649), F4/80(CST,
(6)
Short salsalate administration affects cell proliferation, metabolism, and inflammation in polycystic kidney disease
iScience
November 17, 2023
Kanhai AA, Sánchez-López E, Kuipers TB, van Klinken JB, Dijkstra KL, van der Veen I, Baelde HJ, Song X, Pei Y, Mei H,
et al
.
RRID: AB_442102
Rat
anti-F4/80 primary antibody,Serotec,N/A Rabbit anti-CD3 primary antibody,
Abcam
,
ab16669
; RRID: AB_443425
Rat
anti-
CD19
primary antibody,
ThermoFisher Scientific
,
14-0194-82
; RRID: AB_2637171 anti-
rat
(7)
YAP1/Piezo1 involve in the dynamic changes of lymphatic vessels in UVR-induced photoaging progress to squamous cell carcinoma
Journal of Translational Medicine
November 16, 2023
Yuling L. Yang, Chu Zhou, Qi Chen, Shuzhan Z. Shen, Jiandan D. Li, Xiuli L. Wang, Peiru R. Wang
tissues
labeling
with
LYVE1
(
1:200
;
Abcam
ab219556
),
CD31
(
1:100
; abcam,
ab222783
), CD3 (
1:200
;
Abcam
ab16669
), CD4 (
1:100
; abcam,
ab183635
), VE-CAD (
1:100
, affinity, AF6265), Piezo1 (
1:100
; affinity, DF12083),
(8)
Targeting FAcilitates Chromatin Transcription complex inhibits pleural mesothelioma and enhances immunotherapy
Journal of Experimental & Clinical Cancer Research
November 16, 2023
Anand Singh, Nathanael Pruett, Shivani Dixit, Sudheer K. Gara, Haitao Wang, Roma Pahwa, David S. Schrump, Chuong D. Hoang
Signaling Technology,
9661
), anti-PD-L1,
1:200
(Cell Signaling Technology, 64,988), anti-CD3,
1:200
(
Abcam
,
ab16669
), and anti-
CD8
,
1:200
(
Abcam
,
ab209775
). Pathologic slides were developed with the
horseradish
peroxidase/
(9)
Histological Characteristics of Conjunctiva-Associated Lymphoid Tissue in Young and Adult Holstein Cattle
Animals (Basel)
November 11, 2023
Keigo Kosenda, Osamu Ichii, Yusuke Yamashita, Hiromichi Ohtsuka, Shigeo Fukuda, Yasuhiro Kon
Antibody,Dilution,Blocking,Antigen Retrieval,Secondary Antibody CD3,"Rabbit monoclonal antibodies,
ab16669
(
Abcam
)",
1:800
,10%
NGS
,"TB 115 °C, 15 min","Goat anti-rabbit (SABPO kit, Nichirei)" CD20,"Rabbit polyclonal
(10)
MAP3K19 Affects TWEAK-Induced Response in Cultured Bronchial Epithelial Cells and Regulates Allergic Airway Inflammation in an Asthma Murine Model
Current Issues in Molecular Biology
November 8, 2023
Yuuki Sandhu, Norihiro Harada, Sonoko Harada, Takayasu Nishimaki, Hitoshi Sasano, Yuki Tanabe, Tomohito Takeshige, Kei Matsuno, Ayako Ishimori, Yoko Katsura,
et al.
(Thr183/Tyr185) mAbs were obtained from Cell Signaling Technology (Beverly, MA, USA). Anti-CD3 mAbs (
ab16669
) and anti-MAP3K19 polyclonal antibodies (A14340) were obtained from
Abcam
(Cambridge, MA, USA). Bronchial
(11)
Immune Effects of Cryoablation in Woodchuck Hepatocellular Carcinoma
Journal of Hepatocellular Carcinoma
November 6, 2023
Michal Mauda-Havakuk, Natalie M Hawken, Joshua W Owen, Andrew S Mikhail, Matthew F Starost, Baktiar Karim, Paul G Wakim, Olga L Franco-Mahecha, Andrew L Lewis, William F Pritchard,
et al.
(
ApopTag Peroxidase In Situ Apoptosis Detection Kit
, cat#
S7100
,
Millipore
, Burlington, MA), as well as
CD3
+ (rabbit monoclonal,
ab16669
), CD4+ (rabbit monoclonal,
ab133616
), NCAM (
mouse
monoclonal,
ab9018
), and FOXP3 (rabbit polyclonal,
(12)
Longitudinal evaluation of the biodistribution and cellular internalization of the bispecific CD3xTRP1 antibody in syngeneic mouse tumor models
Journal for ImmunoTherapy of Cancer
October 29, 2023
Gerwin Gerhard Wemke Sandker, Jim Middelburg, Evienne Wilbrink, Janneke Molkenboer-Kuenen, Erik Aarntzen, Thorbald van Hall, Sandra Heskamp
CD8alpha
(
1:4,000
,
ab209775
,
Abcam
) was incubated overnight at 4°C, and rabbit-anti-mouse CD3 (
1:400
,
ab16669
,
Abcam
) for 60 min at RT. Next, secondary antibody
goat
-anti-rabbit/biotin antibody (
1:200
,
BA-1000
,
(13)
TAZ facilitates breast tumor growth by promoting an immune‐suppressive tumor microenvironment
Molecular Oncology
October 16, 2023
Anat Gershoni, Ori Hassin, Nishanth Belugali Nataraj, Sivan Baruch, Adi Avioz‐Seligman, Anna Chiara Pirona, Liat Fellus‐Alyagor, Tomer Meir Salame, Saptaparna Mukherjee, Giuseppe Mallel,
et al.
haematoxylin and eosin (H&E, Sigma, Catalog #HHS332 and Catalog #HT110332), anti‐CD3 (
Abcam
, MA, USA, Catalog #
ab16669
), anti‐
CD8a
(
eBioscience
, CA, USA, Catalog #
14‐0808‐82
), anti‐
FOXP3
(
eBioscience
, Catalog #
14‐5773‐82
),
(14)
Immunomodulatory and pro-oncologic effects of ketamine and isoflurane anesthetics in a murine model
PLOS ONE
October 10, 2023
Dominique Abrahams, Arig Ibrahim-Hashim, Robert S. Ackerman, Joel S. Brown, Christopher J. Whelan, Megan B. Garfinkel, Robert A. Gatenby, Aaron R. Muncey
method was used in Cell Conditioning 1 Mild (Ventana). The primary rabbit antibody that reacts to CD3 (
ab16669
,
Abcam
, Cambridge, MA) was used at a
1:200
dilution in Dako antibody diluent (Carpenteria, CA) and incubated
(15)
Hepatorenal pathologies in TNF-transgenic mouse model of rheumatoid arthritis are alleviated by anti-TNF treatment
Arthritis Research & Therapy
October 2, 2023
Xuefei Li, Yi Wang, Ziqiang Chen, Ming Ruan, Can Yang, Maolin Zhou, Ning Li, Lianping Xing, Hao Xu, Ling Yang,
et al.
AB5320
),
mouse
nephrin antibody (
R&D Systems
,
AF3159
),
anti-CD45R
(
Abcam
,
ab64100
), anti-CD3 (
Abcam
,
ab16669
), anti-iNOS (
Abcam
,
ab3523
), and anti-F4/80 (
Abcam
,
ab6640
). After washing with phosphate buffer, the
(16)
Meta-Analysis of Single-Cell RNA-Seq Data Reveals the Mechanism of Formation and Heterogeneity of Tertiary Lymphoid Organ in Vascular Disease
Arteriosclerosis, Thrombosis, and Vascular Biology
October 1, 2023
Xuejing Sun, Yao Lu, Junru Wu, Qing Wen, Zhengxin Li, Yan Tang, Yunmin Shi, Tian He, Lun Liu, Wei Huang,
et al.
fluorescein isothiocyanate (FITC)–anti-B220 (
11-0460-82
;
eBioscience
, San Diego, CA), rabbit anti-
CD3
(
ab16669
;
Abcam
, Cambridge, MA), mouse anti-CD31 (
910003
;
BioLegend
, San Diego, CA),
rat
anti-MECA-79 (
120801
;
(17)
Microenvironment-dependent growth of Sezary cells in humanized IL-15 mice
Disease Models & Mechanisms
October 1, 2023
Jie Gao, Shumei Ren, Gabrielle Choonoo, Guoying Chen, Davor Frleta, Jun Zhong, Namita Gupta, Prachi Sharma, Adelekan Oyejide, Gurinder S. Atwal,
et al.
paraffin. Sections (4 μm) were cut and stained with
Hematoxylin
and Eosin (H&E), anti-human CD3 (
Abcam
,
ab16669
,
clone
SP7;
1:100
) and 5 μg/ml anti-human PD-1 (
Abcam
,
ab52587
,
clone
NAT105;
1:200
) at 4°C overnight. 5′
(18)
Intratumoral microbial heterogeneity affected tumor immune microenvironment and determined clinical outcome of HBV-related HCC
Hepatology
October 1, 2023
Shengnan Li, Han Xia, Zeyu Wang, Xiehua Zhang, Tianqiang Song, Jia Li, Liang Xu, Ningning Zhang, Shu Fan, Qian Li,
et al.
and immunohistochemistry
staining
. Sections were incubated with monoclonal antibodies against CD3 (
ab16669
,
Abcam
), CD8 (
ab93278
,
Abcam
), CD68 (ZM-0060, ZSBio), CD163 (ZM-0428, ZSBio), and
PD-L1
(
ab210931
,
Abcam
)
(19)
Intranasal inoculation of female BALB/c mice with replication-deficient human adenovirus type 5 expressing SARS-CoV‐2 nucleocapsid protein aggravates lung pathology upon re-encountering the antigen
Virus Research
October 1, 2023
Junxia Cao, Hongjing Gu, Xueting Zhang, Hongfang Yun, Jiarong Li, Chuan-Yimu Si, Jiyan Zhang, Hui Wang
applied in this study include: P-MLKL (
1:100
, Cat#
NBP2-66953
; Novus Biologicals), CD3 (
1:200
, Cat#
ab16669
;
Abcam
),
CD68
(
1:300
, Cat#
97778
;
Cell Signaling
),
CD103
(
1:400
, Cat#
ab224202
;
Abcam
),
Ly6G
(
1:100
,
(20)
Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
Open Veterinary Journal
September 30, 2023
Theerayut Thongrin, Watcharapol Suyapoh, WoroDanur Wendo, Prasarn Tangkawattana, Peerapol Sukon, Kanin Salao, Sutas Suttiprapa, Prasert Saichua, Sirikachorn Tangkawatana
sections were incubated overnight at room temperature with monoclonal rabbit anti-CD3 (dilution
1:200
,
ab16669
,
Abcam plc
, Cambridge, UK). Subsequently, sections were incubated with
goat anti-rabbit IgG H&L (HRP
)
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Reviews
Write a Review
(1)
5/20/2022
Great Anti-CD3 Antibody for Immunofluorescent Staining for Paraffin Tissue
Lucy Liu
We used this antibody to study the T cell infiltration in a TUBO breast cancer mouse model. This anti-CD3 antibody has worked well for immunofluorescent staining for paraffin tissue.
(1)
7/6/2016
A Great Alternative to Mouse Antibodies to Make Your Immunofluorescence Life Easier
Jennifer Marshall
I needed to be able to fit CD3 into my existing antibody panel for immunoflourescence within my tissue. As I study human tissue the antibodies that I was already using were IgG1, 2a, 2b and rat. As I ...
Read Review
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Anti-CD3 antibody [SP7] from Abcam
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