Great Anti-CD3 Antibody for Immunofluorescent Staining for Paraffin Tissue

May 20, 2022

Nanomedicine
Houston Methodist Research Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-CD3 antibody

Catalog Number:

ab16669

Clone Number:

SP7

We used this antibody to study the T cell infiltration in a TUBO breast cancer mouse model. This anti-CD3 antibody has worked well for immunofluorescent staining for paraffin tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

TUBO mouse paraffin slide

Primary Incubation

1:200 overnight

Blocking Agent

5% BSA

Secondary Incubation

1:500 room temperature 2 h

Tertiary Incubation

N/A

Detection

Confocal microscope

Results Summary

Staining works great on paraffin tissue. It's not affected by antigen retrieval step.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

React with Human Rat Mouse sample

The Bad

None

The Bottom Line

Recommend this antibody immunofluorescence staining in mouse paraffin tissues.

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