HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RNF149 (Cat# RC207979, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF149 (Cat# TA810509)(1:2000). Positive lysates [LY406531] (100ug) and [LC406531] (20ug) can be purchased separately from OriGene.