Rb (4H1) Mouse mAb 9309S from Cell Signaling Technology

Product Specs
ItemRb (4H1) Mouse mAb
CompanyCell Signaling Technology
Price $353.00 Inquire
Catalog Number9309S
Quantity100 µl
ApplicationsW, IP, IHC-P, IF-IC, FC-FP, ChIP
ReactivityH, Mk, B, Pg
TargetRb
HostMouse
NCBI Gene AliasesOSRC, PPP1R130, RB, p105-Rb, p110-RB1, pRb, pp110
ClonalityMonoclonal
NCBI Full Gene NameRB transcriptional corepressor 1
Research AreaCell Cycle / Checkpoint Control
Clone4H1
Molecular Weight110

Questions and Answers

Can this antibody be used to study the role of Rb in cancer cell proliferation?

Yes, the Rb (4H1) Mouse mAb has been utilized in various studies to investigate the role of retinoblastoma protein (Rb) in cell cycle regulation and cancer cell proliferation. For instance, it has been referenced in research examining the Notch signaling pathway's influence on Rb and p-AKT in pancreatic cancer cells, highlighting its relevance in understanding tumorigenesis ().

Is this antibody effective for Western blot applications?

The Rb (4H1) Mouse mAb has been shown to work effectively in Western blot applications, as evidenced by its use in multiple studies, including those analyzing the expression of Rb in various cancer models (, ).

What species can this antibody be used on for immunohistochemistry?

The Rb (4H1) Mouse mAb is reactive with several species, including humans, non-human primates, bovines, and porcine tissues, making it suitable for immunohistochemistry across these species ().

Are there any specific mechanisms of action associated with Rb that this antibody can help elucidate?

Yes, the Rb (4H1) Mouse mAb can aid in elucidating mechanisms related to cell cycle checkpoints and apoptosis. For example, it has been used in studies that explore how Rb interacts with other proteins to regulate cell proliferation and survival in cancer contexts, such as its role in the regulation of apoptosis in myeloma cells ().

Cell Signaling Technology

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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The Cyclin C-CDK8/19 Mediator kinase module controls PRCC-TFE3 driven senescence in renal epithelium and tumorigenesis in TFE3-RCCNeoplasiaMay 1, 2026Shoichiro Kuroda, Shintaro Funasaki, Hidekazu Nishizawa, Laura S. Schmidt, Ryoma Kurahashi, Takaaki Ito, Yuichiro Arima, Miwa Tanaka, Atsuya Kitada, Amy M. James, et al.previously [4]. The following primary antibodies were used: TFE3 (ZRB1272; Sigma-Aldrich), Rb (4H1; #9309; Cell Signaling Technology), phospho-Rb (Ser780; #8180; CST), phospho-Rb (Ser807/811; #8516; CST), p16

(2) miR-100-5p Enhances Cell Cycle-Mediated Chemoresistance by Modulating the CTDSPL/pRB/E2F1 Signaling Pathway in Oxaliplatin-Resistant Colorectal Cancer CellsOncology ResearchMarch 23, 2026Yen-Pin Chen, Rathinasamy Baskaran, Hema Sri Devi, Chaouhan Hitesh Singh, Yu-Jung Lin, Marthandam Asokan Shibu, Wei-Wen Kuo, Shih-Chieh Liao, Ming-Cheng Chen, Tso-Fu Wang, et al.(NBP1-91814) (Novus Biologicals Co., Centennial, CO, USA); E2F1 (#3742), phosphorylated RB (#9308), and RB (#9309), p53 (#2524), phosphorylated-p53 (#9284) (Cell Signaling Technology, Inc. Beverly, MA, USA); cyclin

(3) Polyphenol-Rich Extracts from Annurca Apple Differentially Modulate Oxidative Stress-Induced Senescence in Human Dermal FibroblastsAntioxidants (Basel)March 16, 2026Claudia Moriello, Nicola Alessio, Pasquale Perrone, Tiziana Squillaro, Stefania D'Angelo, Umberto Galderisi, Giovanni Di BernardoMembranes were incubated overnight at 4 °C with the following primary antibodies: RB (mw 105 kD) (#9309, CellSignaling, Danvers, MA, USA), P53 (mw 53 kD) (sc-126, SantaCruz Biotechnology, Dallas, TX, USA),

(4) B3GNT3 facilitates NFKB2 processing and non-canonical NF-κB activation to drive lung adenocarcinoma progressionCell Oncol (Dordr)March 16, 2026Ying Lin, Yao Zhang, Bo Yu, Jialei Wang, Zheng Wu, Huijie Wangβ-actin (66009-1-Ig) and tubulin (10094-1-AP). Antibodies against pRB (s807/811, CST, 8516), Rb (CST, 9309) and p100 (s866/870, CST, 4810), p100 (CST, 4882) and H3 (CST, 9715) were purchased from Cell Signaling

(5) An mRNA-encoded scFv antibody targeting the helix-α3 of HPV18 E7 oncoprotein as a novel antiviral strategymBioMarch 11, 2026Feng Han, Xin-ying Guo, Ling-yan Cui, Meng-xuan Zhang, Ya-rong Zeng, Gui-qiang Wang, Jin-jin Li, Xin Chi, Ming-xia Jiang, Yue-ting Xiong, et al.(Abcam, no. ab128131, 1:1,000), anti-β-actin-HRP (ProteinTech, HRP-60008, 1:5,000), anti-Rb (CST, no.9309S, 1:100), anti- phospho-Rb (CST, no.8516S, 1:100). Protein quantification was analyzed using ImageJ. Immunofluorescence Cells

(6) Immunofluorescent microscopy to quantify and characterize chromosomal instability (CIN)Methods in Cell BiologyFebruary 13, 2026Kathryn M. Jones, Yeseo Choi, Radchanon Leelasukseree, Nikki N. Messick, Ava Bryan, Sophie Bice, Pippa F. Cosper(#ab4448, Abcam) cGAS (D1D3G) antibody in rabbit (#15102, Cell Signaling) Rb (4H1) antibody in mouse (#9309, Cell Signaling) STING (D2P2F) antibody in rabbit (#136475, Cell Signaling) Donkey anti-rat IgG

(7) Supercharging-enhanced nDIA-MS enables global profiling of drug-induced proteome solubility shiftsNature CommunicationsJanuary 30, 2026Yun Xiong, Huimin Zhang, Lin Tan, Bo Wei, John N. Weinstein, Philip L. Lorenziincluded SUMO-2/3 (4971S; Cell Signaling Technology), SMAD2 (3103 T; Cell Signaling Technology), RB1 (9309 T; Cell Signaling Technology), EGR1 (4153 T; Cell Signaling Technology), NAB2 (sc-23867, Santa Cruz Biotechnology

(8) Novel Cell Cycle Inhibitors Decrease Primary and Metastatic Breast Cancer Growth In VivoCancers (Basel)January 30, 2026Mir Shahid Maqbool, Yongzhan Zhang, Karin Strittmatter, Ana Gvozdenovic, Simran Asawa, Masroor A. Paddar, Mukesh Kumar, Umed Singh, Parvinder Pal Singh, Nicola Aceto, et al.(p-Rpb1 CTD (Ser2/5) (4735 CS), Rpb1 CTD (2629 CST), p-Rb (Ser807/811) (8516 CST), Rb (4H1) (mouse mAb 9309 CST), and β-actin A3854 (Sigma, Merck KGaA, St. Louis, MO, USA)) and their respective HRP-conjugated

(9) Separate transcription and splicing gene networks are linked and coordinated by the pRb–E2F pathwayNucleic Acids ResearchJanuary 30, 2026Simon M Carr, Geng Liu, Wojciech Barczak, Hasan Syffudin, Shonagh Munro, Denise Reyna-Jeldes, Iolanda Vendrell, Benedikt Kessler, Adam P Cribbs, Alexander Kanapin, et al.(EPR12238, ab204916, Abcam, Cambridge, UK), SRSF2 (PA5-12402, Thermo Fisher, Waltham, USA), pRb (clone 4H1, 9309S, Cell Signaling Technology, Danvers, USA), DP-1 (clone TFD-10, sc-53642, Santa Cruz, Dallas, USA), and

(10) CDK2 inhibitor BLU-222 synergizes with CDK4/6 inhibitors in drug resistant breast cancers through p21/p27 inductionNature CommunicationsJanuary 22, 2026Linjie Luo, Yan Wang, Tuyen Bui, Xiaoting Jiang, Mei-Kuang Chen, Xiayu Rao, Sepideh Mohammadhosseinpour, Mi Li, Serena Kim, Rachel Y. Kim, et al.(H-295; Santa Cruz Biotechnology #sc-753, RRID: AB_2070433; 1:1000), Rb (4H1; Cell Signaling Technology #9309, RRID: AB_823629; 1:1000), phospho-Rb (Ser807/811; Cell Signaling Technology #9308, RRID: AB_331472;

(11) Identification and inhibition of the Cyclin D Rb-docking interface that drives cell divisionbioRxivJanuary 21, 2026Benjamin R. Topacio, Cecelia Brown Fleming, Michael C. Lanz, Shuyuan Zhang, Shicong Xie, Jurgen Tuvikene, Aiden Weaver, Ioannis Sanidas, Julien Sage, Seth M. Rubin, et al.Phospho-Rb (Ser780) (D59B7) Rabbit mAb (Cell Signaling Technology #8180), Rb (4H1) Mouse mAb (Cell Signaling Technology #9309), Monoclonal Anti-α-Tubulin antibody produced in mouse (Sigma-Aldrich T9026), and Rabbit polyclonal

(12) CDK4/6 inhibition overcomes venetoclax resistance mechanisms with enhanced combination activity in acute myeloid leukemiaCell Reports MedicineJanuary 20, 2026Melissa L. Stewart, Jessica Gibbs, Kevin Watanabe-Smith, Ariel Nguyen, Isabel Kenna, Karina Thiel-Klare, Andy Kaempf, Daniel Bottomly, Stephen E. Kurtz, Christopher A. Eide, et al.12D10",Sigma-Aldrich,Cat#MABE343; RRID:AB_2566826 Mouse monoclonal anti-Rb1,Cell Signaling Technology,Cat#9309; RRID:AB_823629 Rabbit monoclonal anti-vinculin,Cell Signaling Technology,Cat#13901; RRID:AB_2728768 anti-human

(13) Therapeutic benefits of maintaining CDK4/6 inhibitors and incorporating CDK2 inhibitors beyond progression in breast cancereLifeDecember 29, 2025Jessica Armand, Sungsoo Kim, Kibum Kim, Eugene Son, Minah Kim, Kevin Kalinsky, Hee Won Yangused to label DNA synthesis. Antibodies Primary antibodies were from Cell Signaling Technology: Rb (9309), phospho-Rb (Ser807/811; 8516), and c-Myc (5605); from Abcam: CDK6 (ab124821), cyclin E (ab32103),

(14) Determinants of sensitivity to HER2-targeted antibody drug conjugates in urothelial cancerNature CommunicationsDecember 20, 2025Ziyu Chen, Xinran Tang, Jordan E. Eichholz, Andrew Mcpherson, Jasmine Thomas, Karan Nagar, Naryan Rustgi, John R. Christin, Fengshen Kuo, Sizhi Gao, et al.HER3 (#12708), ERK (#9102), p-ERK (T202/Y204) (#9101), AKT (#9272), p-AKT (Ser473) (#9271), Rb (4H1) (#9309), GAPDH (#2118), GATA3 (#5852), CD44 (#37259), and KRT14 (#30731), or from Abcam: KRT5 (#52635), and

(15) CDK2 inhibition enhances CDK4/6 inhibitor antitumor activity in comprehensive breast cancer PDX model screenNPJ Breast CancerDecember 3, 2025Nealia C. House, Maxine M. Chen, Sima Khazaei, Victoria Brown, Philip Ramsden, David H. Peng, Sydney Moore, Liang Yuan, Rentian Wu, Fangyang Wang, et al.molecular weight prior to antibody hybridization and detection. Antibodies as follows: Rb1:1000 (CST 9309; RRID:AB_823629), pRbS780 1:1000 (CST 9307; RRID:AB_330015), pRbS807/811 1:1000 (CST 8516; RRID:AB_11178658),

(16) Intrinsic RB activation induces tumoral and stromal anti-tumor responses that limit triple-negative breast cancerNPJ Breast CancerDecember 1, 2025Yin Wan, Jianxin Wang, Thomas N. O'Connor, Vishnu Kumarasamy, Ioannis Sanidas, Scott I. Abrams, Agnieszka K. Witkiewicz, Erik S. KnudsenIC50. Western blotting Primary antibodies for western blotting analysis included RB (4H1) (Cell Signaling, 9309L), pRB (S807/811) (Cell Signaling, 8516S), Cyclin A1 (Sigma, C4710), β-Actin (R&D Systems, MAB8929),

(17) p16 expression confers sensitivity to CDK2 inhibitors in cyclin E1–driven ovarian cancersScience SignalingNovember 25, 2025Chance C. Sine, Lotte P. Watts, Brianna Fernandez, Nasreen Marikar, Jianxin Wang, Erik S. Knudsen, Agnieszka K. Witkiewicz, Sabrina L. SpencerCell Signaling Technology, no. 8516, 1:500, RRID:AB_11178658), Rb (4H1, Cell Signaling Technology, no. 9309, RRID:AB_823629), cyclin E1 (EP435E, Abcam, no. 33911, 1:1000, RRID:AB_731787), pCDK2 Thr160 (Abcam,

(18) RB loss sensitizes triple-negative breast cancer to apoptosis induced by cellular stressCell Death DiscoveryNovember 24, 2025Agnieszka K. Witkiewicz, Subrahmanya Anirudh Kaligotla Venkata, Erik S. Knudsen, Vishnu Kumarasamy(BSA) and 0.5% Triton X-100. Primary antibodies, including anti-RB (RB4H1) (Cell Signaling Technology; 9309 L) and anti- γH2AX (S139) (Cell Signaling Technology; SC-9966), were diluted 1:50 in antibody diluent

(19) Metabolic and imaging phenotypes associated with RB1 and TP53 loss in prostate cancerNeoplasiaOctober 7, 2025Fahim Ahmad, Margaret White, Kazutoshi Yamamoto, Daniel R. Crooks, Supreet Agarwal, Ye Yang, Brian Capaldo, Sonam Raj, Aian Neil Alilin, Anita Ton, et al.milk (Cell Signaling: #9999) in TBST, followed by overnight antibody probing at 4°C°C with RB1 (CST, #9309S), TP53 (Abcam: #PAb240), AR (CST: #5153S), LDH (Novus: #NBP1-48336), KLK3 (CST: #5365), and Histone-H3

(20) Transient APC/C inactivation by mTOR boosts glycolysis during cell cycle entryNatureOctober 2, 2025Debasish Paul, Derek L. Bolhuis, Hualong Yan, Sudipto Das, Xia Xu, Christina C. Abbate, Lisa M. M. Jenkins, Michael J. Emanuele, Thorkell Andresson, Jing Huang, et al.cyclin D1 (Thermo Fisher Scientific, MA5 14512, 1:750), p-Rb (Ser807/811) (CST, 8516, 1:1,000), Rb (CST, 9309, 1:2,000), vinculin (Sigma-Aldrich, V9131, 1:10,000), Ki-67 (Abcam, ab8191, 1:1,000), p21 (BD Biosciences,

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7/10/2018Average Rb Antibody Rebecca WatesWe are interested in mechanisms of resistance to targeted therapies in ovarian cancer. This experiment was designed to determine differential Rb1 protein expression and post-translational ... Read Review

Rb (4H1) Mouse mAb

Rb (4H1) Mouse mAb from Cell Signaling Technology

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Rb (4H1) Mouse mAb from Cell Signaling Technology

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