CHOP (D46F1) Rabbit mAb 5554S from Cell Signaling Technology

Product Specs
ItemCHOP (D46F1) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number5554S
Quantity100 µl
ApplicationsW, W-S, IP
ReactivityM
TargetCHOP
IsotypeIgG
HostRabbit
NCBI Gene AliasesAltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153
NCBI Full Gene NameDNA damage inducible transcript 3
CloneD46F1
Molecular Weight27
ClonalityMonoclonal
Research AreaProtein Folding and Trafficking

Questions and Answers

Can this CHOP antibody be used to study endoplasmic reticulum stress in mouse models?

Yes, the CHOP (D46F1) Rabbit mAb has been utilized in various studies to investigate endoplasmic reticulum stress. For instance, it was employed in a study examining the role of ceramide synthase 1 in oral cancer, where CHOP levels were assessed to understand the relationship between ER stress and tumor progression ().

Is this antibody effective for Western blot applications?

The CHOP antibody has been shown to work effectively in Western blot applications, as evidenced by its use in multiple studies, including those investigating the unfolded protein response and its regulation during cellular stress (, ).

What specific protein does this antibody target, and how is it relevant in cancer research?

This antibody targets the C/EBP homologous protein (CHOP), which is a critical marker of endoplasmic reticulum stress. Its expression is often upregulated in various cancers, indicating its role in apoptosis and tumor progression, as highlighted in studies focusing on the mechanisms of cancer cell survival and resistance to therapies (, ).

Are there any documented effects of CHOP expression on apoptosis in cancer cells?

Yes, increased CHOP expression has been associated with apoptosis in cancer cells. For example, in studies involving hepatocellular carcinoma cells, CHOP was implicated in mediating apoptosis through ER stress pathways, demonstrating its importance in cancer biology ().

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Website: Technical Support

(1) Valosin-Containing Protein Contributes to Plexiform Neurofibroma Formation and Represents a Novel Therapeutic TargetCellsMay 6, 2026Lalitha Gopalan, Youjin Na, Liang Hu, Ashley Hall, Mi-Ok Kim, Eva Dombi, Sara Szabo, Nancy Ratner, Gang Huang, Jianqiang Wu#4695, 1:2000), Ubiquitin (Cell Signaling Technology; #58395, 1:1000), CHOP (Cell Signaling Technology; #5554, 1:1000), GAPDH (Cell Signaling Technology; #3683, 1:3000), or β-actin (Cell Signaling Technology; #5125,

(2) Hadhb Deficiency Inhibits Lung Tumorigenesis Via Activating ER StressJ CancerMay 1, 2026Wuhou Dai, Dong Xu, Yubin Lei, Shujing Zhang, Tonglu Yu, Wufan Tao, Xiaofeng Chen, Xingrong Du(ab198398), ATF6 (ab227830), XBP1 (ab220783) from Abcam; AMPKα (5831), Phospho-AMPKα (Thr172) (50081), CHOP (5554), eIF2α (5324), Phospho-eIF2α (Ser51) (3398), AKT (pan) (4691), Phospho-AKT (4060), Phospho-IkBα (2859),

(3) Toxoplasma gondii effector GRA35 mediates neuronal damage via ER stress and mitochondria-associated apoptosisVirulenceApril 6, 2026Jie Wang, Ying Chen, Nan Zhou, Fangmin Li, Niuniu Dai, Zhiang Chen, Shutong Liu, Ran An, Lijian Chen, Jian Du11815S), GRP78 rabbit polyclonal antibody(Catalog #: 3177S), CHOP rabbit polyclonal antibody (Catalog #: 5554S) all purchased from Cell Signaling Technology. Multicolor prestained protein ladder (Catalog #: WJ103)

(4) REDD1/DDIT4 counteracts endoplasmic reticulum stress-induced apoptosis by controlling the expression of death receptor TRAILR2/DR5 in cancer cellsCell Death & DiseaseMarch 28, 2026Rocío Mora-Molina, Younes El Yousfi, Cathrin Hagenlocher, Francisco Javier Fernández-Farrán, Markus Rehm, Carmen Palacios, Maria A. Christophorou, Abelardo López-RivasTech,11815 Caspase-8,1:1000,Cell Signaling Tech,9746 CtBP,1:2000,Santa Cruz Biotech,sc-17759 CHOP,1:1000,Cell Signaling Tech,5554 FLIP (7F10),1:1000,ENZO,ALX-804-961 GAPDH,1:40000,Santa Cruz Biotech,SC-47724 HRP-linked antibody,1:5000,DAKO,P0448 HRP-linked

(5) XIAP Stabilizes DDRGK1 to Promote ER‐Phagy and Protects Against Noise‐Induced Hearing LossAdvanced ScienceMarch 1, 2026Lin Yan, Yuhua Zhang, Jiawei Du, Yongjun Zhu, Wei Cao, Yongjie Wei, Han Wu, Shiyu Qiu, Shiyi Pan, Lian Chen, et al.(Cell Signaling Technology, 9721); anti‐eIF2α (Cell Signaling Technology, 9722); anti‐CHOP/DDIT3 (Cell Signaling Technology, 5554); anti‐calnexin (Abcam, ab22595); anti‐ATL3 (Proteintech, 16921‐1‐AP); anti‐DDRGK1 (Proteintech, 21445‐1‐AP);

(6) 4-phenylbutyric acid attenuates hepatic ER stress, pyroptosis, and NAFLD in adult rat offspring exposed to a high-fat, high-fructose diet during perinatal and adulthoodLipids in Health and DiseaseFebruary 24, 2026Sara Ojaghi, Farzaneh Eskandari, Fariba Azarkish, Haniyeh Soltani, Fateme Binayi, Behnam Saeidi, Mehdi Hedayati, Afsaneh Goudarzi, Abdollah Amini, Fariba khodagholi, et al.Danvers, MA, USA), BIP (Cat. No.: 3183 S, Cell Signaling Technology, Danvers, MA, USA), CHOP (Cat. No.: 5554 S, Cell Signaling Technology, Danvers, MA, USA) and β-actin (for BIP and CHOP, 1:4000, Cat. No.: 4970,

(7) EGFR inhibitor-resistant lung cancers exhibit collateral sensitivity to a covalent, cysteine-independent KEAP1 oligomerizing molecular bridgeNature CommunicationsJanuary 14, 2026Christopher F. Bassil, Kerry Dillon, Gray R. Anderson, Benjamin Mayro, Kayleigh N. Askin, Peter S. Winter, Stefan Harry, Samuel Gruber, Tierney M. Hall, Jacob P. Hoj, et al.anti-KEAP1 clone 144 (MABS514, Sigma), anti-NRF2 (Ab137550, Abcam), anti-ATF4 (#11815, CST), anti-CHOP (#5554, CST), anti-V5-tag (#13202, CST), anti-GFP (#2555, CST), anti-Vinculin (#4650, CST), and anti-β-Actin

(8) BRCA1 alleviates inflammation, oxidative stress, and ovarian granulosa cell apoptosis by inhibiting endoplasmic reticulum stress, thereby ameliorating polycystic ovary syndromeEuropean Journal of Medical ResearchJanuary 3, 2026Xiaolan Huang, Shuyin Zhang, Danhong Liang, Lingling Qiu, Ruiyun Wu, Rong Wei, Xiaoqing Chen, Suzhen Huang,Cell signaling technology,#9721,1:1000 ATF4,Cell signaling technology,#11815,1:1000 CHOP,Cell signaling technology,#5554,1:1000 β-actin,Abcam,ab8226,1:10000 Goat anti-rabbit IgG,Abcam,ab6721,1:10000 Goat anti-mouse IgG,Abcam,ab205719,1:10000 Information

(9) Reprogramming of cholesterol sensing in epithelial cells supports pancreatic inflammationMolecular MetabolismJanuary 1, 2026Giulia Milan, Olga A. Mareninova, Marco Fantuz, Martina Spacci, Carlotta Paoli, Jerik A. Pineda, Roberta Noè, Beatrice Calciolari, Roberto Zoncu, Anna S. Gukovskaya, et al.(Abcam, #ab40793), amylase (Sigma-Aldrich, #A8273), BiP (Cell Signaling, #3177), CHOP (Cell Signaling, #5554), ERK (Cell Signaling, #9102), phospho-ERK (Cell Signaling, #5726), H3K9Ac (Cell Signaling, #9649),

(10) USP8 inhibits cigarette smoke extract combined with LPS-induced inflammatory response and ER stress by regulating the ABCB1-mediated MAPK signaling pathway in human bronchial epithelial cellsJ Inflamm (Lond)December 29, 2025Meng Wang, Weipeng Song, Xiaoming Liu, Aihong Meng1:600, Invitrogen, Paisley Scotland, UK), USP8 (67321-1-lg, 1:5000, Proteintech, Wuhan, China), COPD (#5554, 1:1000, CST, Danvers, MA, USA), GRP78 (ab108615, 1:1000, Abcam, Cambridge, MA, USA), p38 (#8690, 1:1000,

(11) B-Cell Receptor-Associated Protein 31 Deficiency Aggravates Ethanol-Induced Liver Steatosis and Liver Injury via Attenuating Fatty Acid Oxidation and Glycogen SynthesisInt J Mol SciDecember 18, 2025Shubin Yu, Yaodong Xia, Chunyan Zhang, Xiangyue Han, Xiaoyue Feng, Liya Li, Hang Ma, Jialin Xukit (Beyotime Biotechnology). Antibodies against glucose-regulated protein 78 (GRP78, #3183), CHOP (#5554), p-eIF2α (#3398), inositol-requiring enzyme 1α (IRE1α, #3294), and GAPDH (#5174) were ordered from

(12) Aerobic Exercise Training Exerts Neuroprotective Effects in Alzheimer's Disease Mice by Regulating Endoplasmic Reticulum Stress‐Autophagy Pathway‐Mediated PyroptosisCNS Neuroscience & TherapeuticsNovember 17, 2025Yunliang Wang, Xiangyun Sun, Biao He, Shaowen Yu, Yiyou Yu(Proteintech, Wuhan, Hubei, China, 11587‐1‐AP), rabbit anti‐ATF‐4 (CST, 11815), rabbit anti‐CHOP (CST, 5554), rabbit anti‐Phospho‐PERK (CST, 3179), rabbit anti‐PERK (CST, 3192), rabbit anti‐Phospho‐eIF2α (CST,

(13) Goreisan attenuates cardiac hypertrophy and diastolic dysfunction in heart failure with preserved ejection fraction induced by HFD/L-NAME via regulation of ICAT-β-catenin/ERK axisHypertension ResearchNovember 1, 2025Yoko Shojima Isayama, Shouji Matsushima, Keisuke Shinohara, Koichi Isayama, Nobuyuki Enzan, Taishi Yamamoto, Masashi Sada, Ryo Miyake, Yoshitomo Tsutsui, Takayuki Toyohara, et al.p-NFκB) (Ser536) (CST #3033), NFκB (1:5000, CST #8242), PERK (1:5000, CST #3192), CHOP (1:5000, CST #5554), ICAT (1:1000, sc-293489), β-catenin (1:5000, sc-7963), and glyceraldehyde-3-phosphate dehydrogenase

(14) Inhibition of Histone Deacetylases Induces Cancer Cell Apoptosis Through the PERK Pathway of ER Stress ResponseJ Cell Mol MedOctober 29, 2025Xuan Wang, Caiyun Yang, Yili Yang, Feng Xu, Donglan Yuan, Yuexi Gu(#11815; RRID: AB_2616025), ATF6 (#65880; RRID: AB_2799696), PARP (#9542; RRID: AB_2160739), CHOP (#5554; RRID: AB_10694399), BiP (#3177; RRID: AB_2119845) and IκBα (#9242; RRID: AB_331623) were purchased

(15) Raphin‐1 mediates the survival and sensitivity to radiation of pediatric‐type diffuse high‐grade glioma via phosphorylated eukaryotic initiation factor 2α‐dependent and ‐independent processesMolecular OncologySeptember 1, 2025Karin Eytan, Moshe Leitner, Amos Toren, Shoshana Paglin, Michal Yalonusing imagej software. Rabbit anti‐PeIF2α (9721), anti‐eIF2α (#9722), anti‐BiP (#3177), anti‐CHOP (#5554), anti‐cleaved caspase‐8 (#9496), anti‐DR5 (#8074), and anti‐XBP1s (#40435) were from Cell Signaling Technology

(16) FICD (FIC Domain Protein Adenylyl Transferase) Deficiency Protects Mice From Hypertrophy‐Induced Heart Failure and Promotes BiP (Binding Immunoglobulin Protein) ‐Mediated Activation of the Unfolded Protein Response and Endoplasmic Reticulum‐Selective Autophagy in CardiomyocytesJournal of the American Heart AssociationAugust 19, 2025Shannon M. Lacy, Rebecca J. Taubitz, Nicholas D. Urban, Samantha N. Turowski, Eric N. Primack, Eric D. Smith, Adam S. Helms, Daniel E. Michele, Matthias C. Truttmanncardiac fibroblast staining), BiP (Proteintech, 665741IG, mouse mAb), CHOP (D46F1; Cell Signaling, 5554S, Rabbit mAb), cathepsin B (CtsB; D1C7Y; Cell Signaling, 31718S, Rabbit mAb), cathepsin D (CtsD; E179;

(17) FICD (FIC Domain Protein Adenylyl Transferase) Deficiency Protects Mice From Hypertrophy‐Induced Heart Failure and Promotes BiP (Binding Immunoglobulin Protein) ‐Mediated Activation of the Unfolded Protein Response and Endoplasmic Reticulum‐Selective Autophagy in CardiomyocytesJ Am Heart AssocAugust 18, 2025Shannon M. Lacy, Rebecca J. Taubitz, Nicholas D. Urban, Samantha N. Turowski, Eric N. Primack, Eric D. Smith, Adam S. Helms, Daniel E. Michele, Matthias C. Truttmanncardiac fibroblast staining), BiP (Proteintech, 665741IG, mouse mAb), CHOP (D46F1; Cell Signaling, 5554S, Rabbit mAb), cathepsin B (CtsB; D1C7Y; Cell Signaling, 31718S, Rabbit mAb), cathepsin D (CtsD; E179;

(18) Activation of the integrated stress response and loss of cFLIPL under glutamine limitation induce IL-8 gene expression and secretion in glutamine-dependent tumor cellsCell Death DiscovJuly 19, 2025Rocío Mora-Molina, F. Javier Fernández-Farrán, Abelardo López-Rivas, Carmen PalaciosEnzo Life Sciences (NY, USA). Anti-p-eIF2α (S51) (119A11) (3597), anti-eIF2α (D7D3) (5324), anti-CHOP (D46F1) (5554) and anti-TAK1 (4505) antibodies were purchased from Cell Signaling Technology (CA, USA). Anti-FADD

(19) IFNγ augments TKI efficacy by alleviating protein unfolding stress to promote GSDME-mediated pyroptosis in hepatocellular carcinomaCell Death DisJuly 11, 2025Xiaoxiao Li, Fujia Lu, Jie Zhou, Xiong Li, Yan Li, Weijie Ye, Jing Li, Liguo Yang, Shi Tang, Yuhan Zhou, et al.1:1000, RRID:AB_732117), anti-ATF4 (Santa Cruz, sc-390063, 1:1000,RRID:AB_2810998), anti-CHOP (CST, 5554, 1:1000, RRID:AB_10694399), anti-GADD34 (Proteintech, 10449-1-AP, 1:1000, RRID:AB_2168724), anti-PDIA1

(20) Targeting the self-amplifying loop between ATF6 and SNAI1 to inhibit epithelial‒mesenchymal transition in fibrotic lesionsTheranosticsJuly 11, 2025Tong Wu, Yan Liu, Jiyuan Ma, Lingshan Lu, Liang Wang, Mengmei He, Zhuang Hao, Xiaomin Liu, Luning Zhang, Chao Zhao, et al.(29546-1-AP, Proteintech, China), anti-p-IRE1α (PA1-16927, Thermo Fisher Scientific, MA, USA), anti-CHOP (#5554, Cell Signaling Technology, MA, USA), anti-GRP78 (BIP, 11587-1-AP, Proteintech, China), anti-ATF6 (66563-1-Ig,

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4/5/2017CHOP Rabbit mAb Sachin SamuchiwalI studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's system using anti-mycobacterial ESAT6 binding peptides.

CHOP (D46F1) Rabbit mAb

CHOP (D46F1) Rabbit mAb from Cell Signaling Technology

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CHOP (D46F1) Rabbit mAb from Cell Signaling Technology

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