Rac1/2/3 Antibody 2465S from Cell Signaling Technology

Product Specs
ItemRac1/2/3 Antibody
CompanyCell Signaling Technology
Price $306.00 Inquire
Catalog Number2465S
Quantity100 µl
ApplicationsW
ReactivityH, M, R, Mk, B
HostRabbit
TargetRac2;Rac1;Rac3
NCBI Full Gene NameRac family small GTPase 1, Rac family small GTPase 2, Rac family small GTPase 3
NCBI Gene Aliasesp21-Rac2, HSPC022, TC-25, p21-Rac1, Rac-1, Gx, IMD73B, IMD73C, MRD48, IMD73A, MIG5, EN-7
ClonalityPolyclonal
Research AreaCytoskeletal Signaling
Molecular Weight21

Questions and Answers

Can this antibody be used to study Rac1 activity in cancer cells?

Yes, the Rac1/2/3 Antibody has been utilized in various studies to assess Rac1 activity, particularly in the context of cancer. For instance, it was employed in a study examining the effects of the Rac inhibitor HV-107 on MDA-MB-231 breast cancer cells, where it was used to analyze Rac1 activity through Western blotting techniques ().

Is this antibody effective for detecting Rac proteins in human endothelial cells?

The Rac1/2/3 Antibody has been shown to work effectively in human endothelial cells, as demonstrated in research investigating the interaction between brain-derived neurotrophic factor (BDNF) and endothelial cells. The antibody was used in Western blot analyses to evaluate Rac protein levels in these cells ().

What applications has this antibody been cited in regarding cell migration studies?

The Rac1/2/3 Antibody has been cited in studies focusing on cell migration, particularly in the context of cancer metastasis. For example, it was used to investigate the role of BDNF in enhancing the migratory capabilities of both cancer cells and endothelial cells, highlighting its relevance in migration signaling pathways (; ).

Does this antibody cross-react with other small GTPases?

No, the Rac1/2/3 Antibody is specific and does not cross-react with other small GTPases, making it a reliable choice for studies focused solely on Rac1, Rac2, and Rac3 proteins without interference from other GTPases ().

Cell Signaling Technology

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Protocol to identify covalent inhibitors targeting RhoA Cys16STAR ProtocApril 14, 2026Tin-Yan Koo, Chloe See-Hang Ho, Hillary Yui-Yan Yip, Clive Yik-Sham Chung,Cell Signaling Technology,Cat# 2956S; RRID:AB_1196615 Rac1/2/3 Antibody (Dilution 1:1000 for WB),Cell Signaling Technology,Cat# 2465S; RRID:AB_2176152 Cdc42 Antibody (Dilution 1:1000 for WB),Cell Signaling Technology,Cat# 2462S; RRID:AB_2078085 Anti-rabbit

(2) Context-dependent inhibitory roles of RhoA in 3D invasive cell migration within the extracellular matrixCell ReportsDecember 23, 2025Wakiko Iwata, Yoshihiro Adachi, Junior J. West, Andrew J. Ewald, Yi Zheng, Laura D. Wood, Robert A. Anders, Hiromi Sesaki, Miho Iijimadilution, 2098, Cell Signaling), RHOC (1:1000 dilution, 3430, Cell Signaling), RAC1/2/3 (1:1000 dilution, 2465, Cell Signaling), and CDC42 (1:1000 dilution, 2466, Cell Signaling). Immunocomplexes were detected using

(3) Proteomics-driven screening of artemisinin-based combination ratios and mechanistic insights into Plasmodium berghei infection in miceFront ImmunolDecember 5, 2025Liyu Hao, Jianhui Sun, Jianliang Li, Zongyuan Li, Zeyue Yu, Hanhui Huang, Guimin Liu, Zhenru Shen, Hairu Huo, Qili Yuan, et al.Ser152/156; cat. no.: 2741S); cell division cycle 42 (CDC42; cat. no.: 2462S); RAC1/2/3 (cat. no.: 2465S); all these antibodies were procured from Cell Signaling Technology (USA). Anti-PKC was procured from

(4) Ewing sarcoma depends on C4orf48/NICOL, a NELL2 cofactor regulated by Hippo signalingCellular SignallingOctober 1, 2025Panneerselvam Jayabal, Xiuye Ma, Yidong Chen, Yaxia Yuan, Yuzuru ShiioBioClinova Inc.); mouse monoclonal anti-cdc42 (ACD03, Cytoskeleton); rabbit polyclonal anti-Rac1/2/3 (2465, Cell Signaling Technology); goat polyclonal anti-BRG1 (A303-877A, Bethyl Laboratories); rabbit monoclonal

(5) IGSF10 is a RET antagonist regulating Ewing sarcoma growth and GnRH neuron migrationCell ReportsAugust 26, 2025Panneerselvam Jayabal, Xiuye Ma, Maleeha Akram, Hannah Bow, Pranay Mandal, Naira Mansano, Yidong Chen, Susan T. Weintraub, Peter J. Houghton, Susan Wray, et al.Cell Signaling Technology); mouse monoclonal anti-cdc42 (ACD03, Cytoskeleton); rabbit polyclonal anti-Rac1/2/3 (2465, Cell Signaling Technology); goat polyclonal anti-BRG1 (A303–877A, Bethyl Laboratories); rabbit monoclonal

(6) Plasma exosomes in insulin resistant obesity exacerbate progression of triple negative breast cancerBMC CancerJuly 9, 2025Pablo Llévenes, Andrew Chen, Matthew Lawton, Alejandro N. Rondón-Ortiz, Yuhan Qiu, Michael Seen, Stefano Monti, Gerald V. Denisincubation was performed overnight at 4 °C using Rac1/2/3 antibody (Cell Signaling Technology, Cat# 2465, 1:500 dilution) and β-Actin (13E5) Rabbit mAb (Cell Signaling Technology, Cat# 4970, 1:1000 dilution)

(7) Blocking Nitrosylation Induces Immunogenic Cell Death by Sensitizing NRAS-Mutant Melanoma to MEK InhibitorsCancer ResearchJune 16, 2025Jyoti Srivastava, Vipin K. Yadav, Rachel V. Jimenez, Pravin R. Phadatare, Ninad A. Inamdar, Montana M. Young, Antonella Bacchiocchi, Ruth Halaban, Bin Fang, Alvaro de Mingo. Pulido, et al.(50945), DUSP4 (5149S), pMEK1/2 (9154), S100B (90393), RhoB (2098), RhoA (2117), RhoC (3430S), Rac1/2/3 (2465T), C-Myc (5605S), ATF2 (35031), pGSK3B (9323S), GSK3B (12456S), RSK-1 (8408S), SPRY2 (14954S), AKT (4691),

(8) CD44-downregulation in multiple myeloma inhibits cytoskeleton rearrangement through actin depolymerizationClin Exp MedJune 7, 2025Zhenkui Wang, Yutong Guo, Chun Yang, Hongyan Liang, Jie Zhou, Lei Huang, Yan Xu, Li Xuereagents The following human monoclonal antibodies (mabs) were used: anti-CD44 (Cat:3570 T) and anti-RAC1 (Cat:2465 T) from Cell Signaling, FITC-CD44 (Cat:555478) and APC-CD138 (Cat:347193) from Becton Dickinson, hyaluronic

(9) Novel miRNAs, miR-937-3p, miR-4536-3p, and miR-4650-5p, can Modulate Neuronal Differentiation via the Wnt/MAPK Pathway in SH-SY5Y CellsMolecular NeurobiologyMay 2, 2025Jiyun Choi, Eunjae Jang, Haewon Jeong, Jinsu Hwang, Hyong-Ho Cho, Byeong C. Kim, Geupil Jang, Han-Seong Jeong, Sujeong Jang,Cell Signaling Technology,4695,1:1000 RhoA,Cell Signaling Technology,2117,1:1000 Rac1/2/3,Cell Signaling Technology,2465,1:1000 p-JNK,Cell Signaling Technology,4668,1:1000 JNK,Cell Signaling Technology,9252,1:1000 c-Jun,Cell Signaling Technology

(10) C-reactive protein-induced injury in Mycoplasma pneumoniae -infected lung epithelial cells is mediated by the P38 MAPK/mitochondrial apoptosis pathwayMicrobiology SpectrumMarch 4, 2025Lianjia Li, Yang Zhang, Lin Zhao, Yalin ShiCruz, 56196, 1:200), Bcl-2 (Proteintech #68103–1, 1:500), Bax (Proteintech, 50599–2, 1:400), Rac1 (CST, 2465, 1:1000), Phospho-Rac1 (Merck Millipore, 07–896, 1:100), ACTIB (Abcam, ab8227), TAK1 (Abcam, ab109526,

(11) Discoidin domain receptor inhibitor DDR1-IN-1 induces autophagy and necroptotic cell death in malignant peripheral nerve sheath tumorCell Death DiscovMarch 1, 2025Guan-Yi Lai, Yu-Cheng Lee, Hao-Jui Weng, Kuei-Hung Lai, Min-Chen Hsiang, Kai-Yu Hsu, Chung-Ping Liao(Ser371) (Cell Signaling, 9208), Phospho-4E-BP1 (Thr37/46) (Cell Signaling, 2855), CDK2 (Cell Signaling, 2465), CDK6 (Cell Signaling, 3136), Cyclin D1 (Cell Signaling, 2978), CDK4 (Cell Signaling, 12790), DDR1

(12) Loss of PRICKLE1 leads to subfertility, aberrant extracellular matrix, and abnormal myometrial architecture in miceReproductionMarch 1, 2025Emily R Roberts, Sornakala Ganeshkumar, Sumedha Gunewardena, Vargheese Chennathukuzhianti-p-PKC (Cell Signaling Technology, Cat# 9379S, 1:1000), anti-RAC1/2/3 (Cell Signaling Technology, Cat# 2465P, 1:1000), anti-RHOA (Cell Signaling Technology, Cat# 2117P, 1:1000), anti-WNT5A (Cell Signaling Technology,

(13) Noncanonical Wnt/Ror2 Signaling Regulates Basal Cell Fidelity and Branching Morphogenesis in the Mammary GlandbioRxivFebruary 28, 2025Hongjiang Si, Erika Mendoza Mendoza Mendoza, Madelyn Esquivel, Chad J. Creighton, Jianming Xu, Kevin RoartyCell Signaling Technology [CST], Danvers, MA), ROCK1 (1:1000; #4035; CST), Cdc42 (1:1000; #2466; CST), Rac1/2/3 (1:1000; #2465; CST), YAP/TAZ (1:1000; #8418; CST), phosphorylated YAP (p-YAP, 1:1000; #13008; CST), LATS1 (1:1000;

(14) Pharmacological manipulation of TRPC5 by kaempferol attenuates metastasis of gastrointestinal cancer via inhibiting calcium involved in the formation of filopodiaInternational Journal of Biological SciencesSeptember 16, 2024Suyun Yu, Rui Deng, Wei Wang, Defang Zou, Liang He, Zhonghong Wei, Yanhong Pan, Xiaoman Li, Yuanyuan Wu, Aiyun Wang, et al.(Cell Signaling Technology, 2466), RhoA (Cell Signaling Technology, 2117), Rac1/2/3 (Cell Signaling Technology, 2465), p-myosin light chain (p-MLC, Ser19) (Cell Signaling Technology, 3671T), p-cortactin (Tyr421) (Affinity,

(15) Multi-scale photocatalytic proximity labeling reveals cell surface neighbors on and between cellsScienceJuly 19, 2024Zhi Lin, Kaitlin Schaefer, Irene Lui, Zi Yao, Andrea Fossati, Danielle L. Swaney, Ajikarunia Palar, Andrej Sali, James A. Wells(Cell Signaling Technology, 4775S), anti-CKAP4 (Proteintech, 16686–1-AP), anti-Rac1/2/3 (Cell Signaling Technology, 2465T), anti-DOCK4 (Proteintech, 21861–1-AP), anti-SIGMAR1 (Proteintech, 15168–1-AP), anti-PKACa (Cell Signaling Technology

(16) Excessive endometrial PlGF- Rac1 signalling underlies endometrial cell stiffness linked to pre-eclampsiaCommunications BiologyMay 4, 2024Janet P. Raja Xavier, Carmela Rianna, Emily Hellwich, Iliana Nikolou, Aditya Kumar Lankapalli, Sara Y. Brucker, Yogesh Singh, Florian Lang, Tilman E. Schäffer, Madhuri S. Salkerthe membrane was washed twice with TBS-T and incubated with primary antibodies against Rac1 (1:1000, #2465 s, Cell Signaling Technology), phospho Rac1 (1:500, #2461 S, Cell Signaling Technology), PAK1 (1:500,

(17) The fusion gene LRP1–SNRNP25 drives invasion and migration by activating the pJNK/37LRP/MMP2 signaling pathway in osteosarcomaCell Death DiscoveryApril 27, 2024Peipei Xing, Haotian Liu, Wanyi Xiao, Gengpu Zhang, Chao Zhang, Zhichao Liao, Ting Li, Jilong Yangphospho-STAT3 (Abcam#ab76315, 1:1000), phospho-AKT (CST#9271S, 1:1000), AKT (CST#9272S, 1:1000), Rac1/2/3 (CST#2465S, 1:1000), RhoA (CST#2117S, 1:1000), phospho-JNK (CST#9251S, 1:1000), JNK (CST#9252T, 1:1000), MMP2 (CST#4022S,

(18) PRL2 regulates neutrophil extracellular trap formation which contributes to severe malaria and acute lung injuryNature communicationsJanuary 29, 2024Du X, Ren B, Li C, Li Q, Kan S, Wang X, Bai W, Wu C, Kassegne K, Yan H, et al.05-1583, clone 42), GAPDH antibody (1:1000, Proteintech, 10494-1-AP), Rac1/2/3 antibody (1:1000, CST, 2465), HRP linked anti mouse IgG (1:2000, CST, 7076) and HRP linked anti rabbit IgG (1:2000, CST, 7074). Rac

(19) Roles of USP1 in Ewing sarcomaGenes & cancerJanuary 1, 2024Jayabal P, Ma X, Shiio Y.MilliporeSigma); mouse monoclonal anti-cdc42 (ACD03, Cytoskeleton); rabbit polyclonal anti-Rac1/2/3 (2465, Cell Signaling Technologies); goat polyclonal anti-BRG1 (A303-877A, Bethyl Laboratories); rabbit monoclonal

(20) Signaling-induced systematic repression of miRNAs uncovers cancer vulnerabilities and targeted therapy sensitivityCell reports. MedicineOctober 17, 2023Wurm AA, Brilloff S, Kolovich S, Schäfer S, Rahimian E, Kufrin V, Bill M, Carrero ZI, Drukewitz S, Krüger A, et al.,Cell Signaling Technology,Cat# 4795; RRID: AB_2099883 Rac1/2/3 Antibody,Cell Signaling Technology,Cat# 2465; RRID: AB_2176152 GAPDH (D16H11) XP Rabbit mAb,Cell Signaling Technology,Cat# 5174; RRID: AB_10622025 Mouse

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Rac1/2/3 Antibody

Rac1/2/3 Antibody from Cell Signaling Technology

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Cell Signaling Technology

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Rac1/2/3 Antibody from Cell Signaling Technology

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