EGR1 (44D5) Rabbit mAb 4154S from Cell Signaling Technology

Product Specs
ItemEGR1 (44D5) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number4154S
Quantity100 µl
ApplicationsW, IP, IF-IC, FC-FP, ChIP, ChIP-seq
ReactivityH, M, R
TargetEGR1
HostRabbit
NCBI Gene AliasesAT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
ClonalityMonoclonal
NCBI Full Gene Nameearly growth response 1
Research AreaNeuroscience
Clone44D5
Molecular Weight75

Questions and Answers

What applications has the EGR1 (44D5) Rabbit mAb been used for in scientific research?

The EGR1 (44D5) Rabbit mAb has been shown to work in various applications including Western Blotting, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, and ChIP-seq.

Can this antibody be used to study EGR1's role in macrophage function during infections?

Yes, the EGR1 antibody has been utilized in studies investigating its role in macrophage phagocytosis during Pseudomonas aeruginosa infection, where it was found that EGR1 suppresses phagocytosis by inhibiting NRF2 activation through upregulation of autophagy ().

Is the EGR1 antibody effective in cancer research, particularly in breast cancer?

The EGR1 antibody has been used in breast cancer research, where it was shown to be required for T-box 2 to repress NDRG1, a tumor suppressor gene, thereby driving the proliferation of breast cancer cells ().

How has the EGR1 antibody been applied in studies related to memory and learning?

The EGR1 antibody has been employed in research examining its role in memory processes, specifically in the context of hippocampal function, where it was implicated in the regulation of contextual fear memory formation ().

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Website: Technical Support

(1) The plant hormone, 6-benzylaminopurine, ameliorates obesity in male and female mice while on a high-fat dietMol MetabApril 11, 2026Calvin V. Lieu, Cindy X. Zhang, Neruja Loganathan, Leon French, Andre Krunic, Sarah B. Cash, Jin Shi, Juliette Lee, Kacey J. Prentice, Carolyn L. Cummins, et al.phospho-p70-S6K (CST, cat. # 9205), p70-S6K (CST, cat. # 9202), INSR (CST, cat. # 3025), and EGR1 (CST, cat. # 4154). 4.10 Immunocytochemistry (ICC) mHypoE-46 cells were cultured in 8-well chamber slides in DMSM supplemented

(2) HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvementFrontiers in Cell and Developmental BiologyJanuary 14, 2026Xuepeng Bian, Qinghui Shang, Lutao Zhu, Jun Liu, Min Wu, Jingjing Li, Shujie Lou(3489S, CST), P90RSK (9355S, CST), ERK (4370T, CST), CREB (9197S, CST), c-Fos (2250S, CST), Zif268 (4154S, CST), NMDAR (5704S, CST), SYN (4329S, CST), BDNF (28205-1-AP, Proteintech), ARC (16290-1-AP, Proteintech),

(3) Early growth response 1 promotes RNA polymerase I-directed transcription and cancer growth by activating RRN3 expressionCommunications BiologyJanuary 9, 2026Xiaoye Song, Wenxin Xia, Jiandong Zhang, Zhongyu Wu, Huating Zeng, You Yang, Ye Wang, Deen Yu, Shasha Zhao, Baoqiang Guo, et al.Immunofluorescence (IF) staining was subsequently performed with anti-EGR1 antibodies (ab194357; Abcam and Cat no. 4154S; CST) and antibodies against Nucleophosmin (ab183340; Abcam), UBF (ab244287; Abcam), RRN3 (SC-390464;

(4) Sphingosine kinase 1 is integral for elastin deficiency-induced arterial hypermuscularizationNature Cardiovascular ResearchJanuary 5, 2026Junichi Saito, Jui M. Dave, Eunate Gallardo-Vara, Nandhini Sadagopan, Inamul Kabir, George Tellides, Robert K. Riemer, Zsolt Urban, Sarah Spiegel, Timothy Hla, et al.caspase-3 (Cell Signaling Technology, 9664; 1:250 dilution), rabbit anti-EGR1 (Cell Signaling Technology, 4154; 1:1,000 dilution), rabbit anti-S1PR1 (Proteintech, 55133–1-AP; 1:200 dilution), chicken anti-GFP (Abcam,

(5) EpCAM activates the ERK-EGR1 signaling axis and promotes TNF-α-induced the progression of anaplastic thyroid cancerJournal of Translational MedicineDecember 22, 2025Chi-Chiu Lee, Yi-Jen Su, Sushree Shankar Panda, Chiung-Yi Chiu, Chung-Hsuan Wu, Tsai-Ming Lu, Pi-Chun Li, Yen-Lin Huang, Ruey-Long Hong, Shao-Hsi Hung, et al.(#2840), E-cadherin (#3195), Vimentin (#5741), Snail (#3879), Slug (#9585), TNF-α (#11948), and EGR1 (#4154) were obtained from Cell Signaling Technology. Antibodies against human α-tubulin (T5168) and β-tubulin

(6) SH3BGRL2 as a vital tumor suppressor and prognostic factor in human esophageal squamous cell carcinomaJournal of Thoracic DiseaseOctober 31, 2025Kaiyan Chen, Rui Zhu, Fanrong Zhang, Jiaoyue Jin, Minran Huang, Lisha Ying, Yun Fan, Dan Su, Hongchuan Jin#2146; CST), SH3BGRL2 (1:1,000; #21944-1-AP; Proteintech), and early growth response 1 (EGR1; 1:500, #4154; CST). The membranes were washed three times with Tris-buffered saline with Tween20 (TBST) and incubated

(7) PDGF-BB/EGR1 Axis Drives Fibroblast Activation Protein Expression to Promote Abdominal Aortic AneurysmInt J Med SciJune 5, 2025Zhihao Zhou, Lin Huang, Hui Luo, Rongzhou He, Ridong Wu, Rui Wang, Kangjie Wang, Chen Yao(HY-P7087, respectively, MCE). The primary antibodies for FAP (bs-5758R, Bioss, ab207178, abcam), EGR1 (4154, Cell Signaling Technology), ACTA2(67735, Proteintech), F4/80 (28463, Proteintech), CD68 (1:500, 76437,

(8) Image-based identification and isolation of micronucleated cells to dissect cellular consequencesElifeJune 2, 2025Lucian DiPeso, Sriram Pendyala, Heather Z Huang, Douglas M Fowler, Emily M Hatchrabbit-a-ATF3 (1:400; Cell Signaling Technology, 18665), rabbit-a-EGR1 (1:1600; Cell Signaling Technology, 4154), and rabbit α H3K27Ac (1:500; Abcam, ab4729). Secondary antibodies used were: AF647 goat-a-mouse (1:1000;

(9) Long-term expansion of basal cells and the novel differentiation methods identify mechanisms for switching Claudin expression in normal epitheliaSci RepApril 9, 2025Akihito Inoko, Norihito Soga, Minako Suzuki, Tohru Kiyono, Junichi Ikenouchi, Takahiro Kojima, Yoshikatsu Sato, Daisuke Saito, Tatsuo Miyamoto, Naoki Goshima, et al.(21050-1-AP, Proteintech), rabbit anti-MUC1/EMA C-terminal (23614-1-AP, Proteintech), rabbit anti-EGR1 (#4154, Cell Signaling Technology, Massachusetts, USA), rabbit anti-ELF3 (HPA003479, Sigma), mouse anti-GRHL3

(10) NNMT promotes acquired EGFR-TKI resistance by forming EGR1 and lactate-mediated double positive feedback loops in non-small cell lung cancerMol CancerMarch 15, 2025Jiali Dai, Xiyi Lu, Chang Zhang, Tianyu Qu, Wei Li, Jun Su, Renhua Guo, Dandan Yin, Pingping Wu, Liang Han, et al.ABclonal. The specific antibody for H3K18la was purchased from PTM. The specific antibody for EGR1 (Cat. # 4154) was purchased from Cell Signaling Technology. The ChIP primer sequences are listed in Table S3. The

(11) Image-based identification and isolation of micronucleated cells to dissect cellular consequencesbioRxivJanuary 7, 2025Lucian DiPeso, Sriram Pendyala, Heather Z. Huang, Douglas M. Fowler, Emily M. Hatchrabbit-a-ATF3 (1:400; Cell Signaling Technology, 18665), rabbit-a-EGR1 (1:1600; Cell Signaling Technology, 4154) and rabbit α H3K27Ac (2 μg/mL; Abcam, ab4729). Secondary antibodies used were: AF647 goat-a-mouse (1:1000;

(12) RNA Shielding of p65 Is Required to Potentiate Oncogenic Inflammation in TET2-Mutated Clonal HematopoiesisCancer discoveryDecember 2, 2024Ben-Crentsil NA, Mohammed Ismail W, Balasis ME, Newman H, Quintana A, Binder M, Kruer T, Neupane S, Ferrall-Fairbanks MC, Fernandez J, et al.(Cell Signaling Technology Cat# 2729, RRID:AB_1031062) and the other with EGR1 antibody (Cell Signaling Technology Cat# 4154, RRID:AB_2097035) overnight at 4°C with rotation. Magnetic beads were then added and allowed to incubate

(13) Myeloid PTEN loss affects the therapeutic response by promoting stress granule assembly and impairing phagocytosis by macrophages in breast cancerCell Death DiscoveryJuly 30, 2024Yan Li, Chao Xu, Xiaojun Qian, Gang Wang, Chaoqiang Han, Hui Hua, Menghao Dong, Jian Chen, Haiyang Yu, Rutong Zhang, et al.1:1000 for WB), anti-FMR1 (Proteintech, 13755-1-AP, 1:3000), anti-EGR1 (Cell Signaling Technology, 4154 S, 1:1000 for WB and 1:50 for Chip-seq), anti-CD68 (Cell Signaling Technology, 76437 S, 1:400), anti-DDX3

(14) Phospholipase C beta 1 in the dentate gyrus gates fear memory formation through regulation of neuronal excitabilityScience advancesJuly 5, 2024Lee J, Jeong Y, Park S, Kim S, Oh H, Jin JA, Sohn JW, Kim D, Shin HS, Heo WD.anti-Cre recombinase (1:1000; Abcam, ab190177), anti-cFos (1:1500; CST, #2250), anti-Egr1 (1:1000; CST, #4154), and anti-Arc (1:1500; SYSY, 15600). The secondary antibodies were as follows: anti-Rat immunoglobulin

(15) miR-124-3p downregulates EGR1 to suppress ischemia-hypoxia reperfusion injury in human iPS cell-derived cardiomyocytesScientific ReportsJune 27, 2024Qiaoke Yang, Kozue Murata, Tadashi Ikeda, Kenji Minatoya, Hidetoshi Masumotomilk TBST buffer for 1 h, followed by incubation with the primary antibodies: EGR1 (44D5) Rabbit mAb (#4154, 75 kDa, 1:1000, Cell Signaling Technology, Danvers, MA, USA), BCL2 (D17C4) Rabbit mAb (#3498, 26 kDa,

(16) Early growth response 1 regulates dual‑specificity protein phosphatase 1 and inhibits cell migration and invasion of tongue squamous cell carcinomaOncology LettersApril 3, 2024Longxun Zhou, Yuqun Shan, Jun Li, Min Li, Zhen Meng, Na Guointo fragments of 200–1,000 bp. The fragments were then incubated with 5 µg Egr-1 antibody (cat. no. 4154; Cell Signaling Technology, Inc.) at 4°C for 12 h and pulled down using Protein A/G magnetic beads (cat.

(17) Rapid unleashing of macrophage efferocytic capacity via transcriptional pause releaseNatureApril 1, 2024Tufan T, Comertpay G, Villani A, Nelson GM, Terekhova M, Kelley S, Zakharov P, Ellison RM, Shpynov O, Raymond M, et al.1:1,000; β-actin (clone AC-15), Sigma, A3854, 1:30,000; EGR1 (clone 44D5), Cell Signaling Technology, 4154, 1:1,000; EGR3 (clone A-7), Santa Cruz, sc-390967, 1:1,000; EGR3, antibodies-online, ABIN970600, 1:500,

(18) Egr-1 is a key regulator of the blood-brain barrier damage induced by meningitic Escherichia coliCell communication and signaling : CCSJanuary 17, 2024Yang R, Wang X, Liu H, Chen J, Tan C, Chen H, Wang X.serum albumin before being incubated with primary antibodies against Egr-1 (Cell Signaling Technology, 4154, 1:1000, Danvers, MA, USA), Claudin-5 (Abcam, ab131259, 1:2000, Cambridge, MA, USA), vascular cell adhesion

(19) Chitotriose Enhanced Antitumor Activity of Doxorubicin through Egr1 Upregulation in MDA-MB-231 CellsMarine DrugsDecember 29, 2023Heng Li, Ke Ji, Peng Liu, Yan Geng, Jinsong Gong, Chao Zhang, Zhenzhong Ding, Zhenghong Xu, Jinsong Shi(Santa Cruz, CA USA). The antibodies for early growth response protein 1 (EGR1) (catalogue number: 4154S), growth arrest, and the DNA damage-inducible protein alpha (GADD45A) (catalogue number: 4632S), and

(20) Lactate Mediates High-Intensity Interval Training-Induced Promotion of Hippocampal Mitochondrial Function through the GPR81-ERK1/2 PathwayAntioxidants (Basel)December 7, 2023Qinghui Shang, Xuepeng Bian, Lutao Zhu, Jun Liu, Min Wu, Shujie LouMA, USA), t-ERK1/2 (4695, CST), Arc (16290-1-AP, Proteintech, Wuhan, China), c-Fos (2250, CST), Egr1 (4154, CST), BDNF (28205-1-AP, Proteintech), SYN (4329, CST), PSD95 (3450, CST), PGC-1α (NBP1-04676SS, Novus,

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3/7/2025This EGR1 Antibody is an Excellent Choice for Accurately Detecting EGR1 in Pancreatic Islets Through IHC YONG MJun B was studied in mouse pancreatic islets. The results indicated that EGR1 can be detected in the cell nucleus.

EGR1 (44D5) Rabbit mAb

EGR1 (44D5) Rabbit mAb from Cell Signaling Technology

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EGR1 (44D5) Rabbit mAb from Cell Signaling Technology

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