Argonaute 1 (D84G10) XP ® Rabbit mAb 5053S from Cell Signaling Technology

Product Specs
ItemArgonaute 1 (D84G10) XP ® Rabbit mAb
CompanyCell Signaling Technology
Price $371.00 Inquire
Catalog Number5053S
TargetAgo1
HostRabbit
NCBI Gene AliasesEIF2C, EIF2C1, GERP95, NEDLBAS, Q99, hAgo1
ClonalityMonoclonal
NCBI Full Gene Nameargonaute RISC component 1
ApplicationsWB, IHC-p, IP
ReactivityHu, Ms, Rt, Mk
Research AreaTranslational Control
IsotypeIgG
CloneD84G10
Molecular Weight97
Quantity100 µl

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Argonaute 1 contributes to the transcriptional silencing of HIV-1The Journal of biological chemistryOctober 1, 2025Goudey S, Ait Said M, Desforges J, Marie S, Morel M, Berlioz-Torrent C, Margottin-Goguet F, Emiliani S, Matkovic R, Gallois-Montbrun S.at 4 °C with the specified primary antibodies: anti-Ago1 4B8 (SAB4200084; Sigma), anti-Ago1 D84G10 (5053; Cell Signaling), anti-Ago2 C34C6 (2897; Cell Signaling), anti-Dicer A-2 (sc-136981; Santa Cruz), anti-Drosha

(2) Nuclear Argonaute:miRNA complexes recognize target sequences within chromatin-associated RNA and silence gene expressionNucleic Acids ResAugust 27, 2025Cristina R Hofman, Jiaxin Hu, Rut Bryl, Victor Tse, David R Coreyantibodies in blocking buffer at 4°C on rocking platform overnight: anti-AGO1 1:1000 (Cell Signaling, #5053), anti-AGO2 1:2000 (Fujifilm Wako, 015-22031), anti-AGO3 1:1000 (Cell Signaling, #5054), anti-AGO4 1:1000

(3) C-terminal tagging impairs AGO2 functionRNA BiolJuly 23, 2025Kunal M. Shah, Alex F. F. Crozier, Anika Assaraf, Muzjda Arya, Paul Grevitt, Faraz Mardakheh, Michael J. Plevin, Tyson V. Sharpanti-beta-Actin (Sigma #A1978), mouse anti-Vinculin (Invitrogen, 14–9777–82), rabbit anti-AGO1 (CST, 5053), rabbit anti-AGO2 (CST, 2897), rabbit anti-AGO2 (Sino Biological 101,620-T36), rabbit anti-AGO3 (Abcam

(4) Repression of AGO1 by AGO2 via let-7 microRNAs facilitates embryonic stem cell differentiationRNAJune 1, 2025Gabrielle M. Schuh, Katharine R. Maschhoff, Annastasia Minor, Wenqian Hu(pX458),Addgene,48138 Antibodies,Antibodies,Antibodies Rabbit monoclonal anti-Ago1 (D84G10),Cell Signaling Technology,Cat# 5053 Rabbit monoclonal anti-Ago2 (C34C6),Cell Signaling Technology,Cat# 2897 Mouse monoclonal anti-GAPDH

(5) Nuclear AGO2 supports influenza A virus replication through type-I interferon regulationNucleic Acids ResApril 12, 2025Hsiang-Chi Huang, Michelle Fong, Iwona Nowak, Evgeniia Shcherbinina, Vivian Lobo, Danica F Besavilla, Hang T Huynh, Karin Schön, Jakub O Westholm, Carola Fernandez, et al."FLAG (Merck, M8823)" "HA (Sigma, H9658)" "AGO1 (CST, 5053S)" "AGO2 (Abcam, ab32381)" "AGO2 11A9 for IF (Merck, MABE253)" "AGO3 (CST, 5054)" "β-Tubulin (Proteintech,

(6) SART3 reads methylarginine-marked glycine- and arginine-rich motifsCell reportsJuly 23, 2024Wang Y, Zhou J, He W, Fu R, Shi L, Dang NK, Liu B, Xu H, Cheng X, Bedford MT.AB_2879148 PRPF6,Proteintech,Cat# 23929–1-AP; RRID: AB_2879365 AGO1,Cell Signaling Technology,Cat# 5053; RRID: AB_2616013 SNRPB,Santa Cruz Biotechnology,Cat# sc-271094; RRID: AB_10611786 La,Bethyl,Cat#

(7) Characterization of Argonaute-containing protein complexes in Leishmania-infected human macrophagesPLOS ONEMay 23, 2024Atieh Moradimotlagh, Harsimran Kaur Brar, Stella Chen, Kyung-Mee Moon, Leonard J. Foster, Neil Reiner, Devki NandanAmicon® Ultra-2 centrifugal filter devices (UFC200324) were purchased from Millipore Sigma. Anti-Ago1 (5053), anti-Ago2 (2897), anti-Ago3 (5054), anti-Lamin A/C (2032), anti-Calreticulin (12238), and anti-Calnexin

(8) Leishmania infection upregulates and engages host macrophage Argonaute 1, and system-wide proteomics reveals Argonaute 1-dependent host responseFrontiers in ImmunologyNovember 30, 2023Atieh Moradimotlagh, Stella Chen, Sara Koohbor, Kyung-Mee Moon, Leonard J. Foster, Neil Reiner, Devki Nandanthe appropriate antibodies, according to the manufacturer’s instructions: Anti-Ago1 (Cell Signaling 5053), anti-Ago2 (Cell Signaling 2897), anti-GST (Santa Cruz sc-138), anti-GAPDH (Applied Biological Materials

(9) The length of uninterrupted CAG repeats in stem regions of repeat disease associated hairpins determines the amount of short CAG oligonucleotides that are toxic to cells through RNA interferenceCell death & diseaseDecember 30, 2022Murmann AE, Patel M, Jeong SY, Bartom ET, Jennifer Morton A, Peter ME.RRID:AB_626632), anti-human AGO2 (Abcam #AB186733, RRID:AB_2713978). anti-human AGO1 (D84G10, Cell Signaling, #5053) and anti-human DICER Rabbit mAb (D38E7, Cell Signaling #5362). Secondary antibodies for Western blot:

(10) Global and precise identification of functional miRNA targets in mESCs by integrative analysisEMBO reportsSeptember 5, 2022Schaefer M, Nabih A, Spies D, Hermes V, Bodak M, Wischnewski H, Stalder P, Ngondo RP, Liechti LA, Sajic T, et al.Cat#D28B1), DICER antibody 1:2,000 (Sigma Aldrich; SAB4200087), AGO1 antibody 1:1,500 (Cell Signaling; Cat#5053), AGO2 antibody 1:1,500 (Cell Signaling; Cat#2897S), TFAP4 antibody 1:1,000 (ab223771; Abcam), TUBULIN antibody

(11) Mixed Ligand Mononuclear Copper(II) Complex as a Promising Anticancer Agent: Interaction Studies with DNA/HSA, Molecular Docking, and In Vitro Cytotoxicity StudiesACS OmegaJune 28, 2022Kumudini Paliwal, Paramita Haldar, P. K. Sudhadevi Antharjanam, Manjuri KumarAntibodies, namely, anti-p53 (cat. no. 2527T), anti-caspase 3 (14220T), anti-beta actin (4970T), anti-bax (5053T), and goat horseradish peroxidase-linked anti-rabbit (7074P) were purchased from Cell Signaling Technology.

(12) Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophyCellJune 9, 2022Marasco LE, Dujardin G, Sousa-Luís R, Liu YH, Stigliano JN, Nomakuchi T, Proudfoot NJ, Krainer AR, Kornblihtt AR.(Proteintech, 15606-1-AP), anti-actin (Sigma-Aldrich, A2066), anti-G9A (CST, 3306S) or anti-Ago1 (CST, 5053S) antibodies. RNAi knockdown Downregulation of hnRNP A1 and A2 was performed using ON-TARGET plus SMARTpool

(13) AGO1 regulates pericentromeric regions in mouse embryonic stem cellsLife Science AllianceJune 1, 2022Madlen Müller, Tara Fäh, Moritz Schaefer, Victoria Hermes, Janina Luitz, Patrick Stalder, Rajika Arora, Richard Patryk Ngondo, Constance Ciaudodiluted in blocking solution at 4°C. Primary antibodies used were: HP1α (#2616, 1:2,000; CST), AGO1 (#5053, 1:2,000; CST), LAMIN B1 (ab16048, 1:10,000; Abcam), TUBULIN (T6199, 1:10,000; Sigma-Aldrich), H3K9me3

(14) microRNA-mediated regulation of microRNA machinery controls cell fate decisionseLifeOctober 1, 2021Qiuying Liu, Mariah K Novak, Rachel M Pepin, Taylor Eich, Wenqian Huanti-beta-Tubulin,Selleckchem,Cat# A5032,WB (1:5000) Antibody,(Rabbit monoclonal) anti-Ago1 (D84G10),Cell Signaling Technology,Cat# 5053,WB (1:1000) Antibody,(Rabbit monoclonal) anti-Ago2,Bimake,Cat# A5701,WB (1:3000) Antibody,(Mouse monoclonal)

(15) microRNA-mediated regulation of microRNA machinery controls cell fate decisionsElifeOctober 1, 2021Qiuying Liu, Mariah K Novak, Rachel M Pepin, Taylor Eich, Wenqian Huanti-beta-Tubulin,Selleckchem,Cat# A5032,WB (1:5000) Antibody,(Rabbit monoclonal) anti-Ago1 (D84G10),Cell Signaling Technology,Cat# 5053,WB (1:1000) Antibody,(Rabbit monoclonal) anti-Ago2,Bimake,Cat# A5701,WB (1:3000) Antibody,(Mouse monoclonal)

(16) LncRNA PVT1 promotes the progression of ovarian cancer by activating TGF‐β pathway via miR‐148a‐3p/AGO1 axisJournal of Cellular and Molecular MedicineSeptember 1, 2021Yuxian Wu, Wenqian Gu, Xiao Han, Zhijun Jinovernight at 4°C. The following antibodies and dilutions were used: AGO1 (Cell Signaling, Danvers, USA, 5053; 1:1000 dilution), E‐cadherin (Cell Signaling, 14472; 1:1000 dilution), N‐cadherin (Cell Signaling,

(17) Argonaute binding within human nuclear RNA and its impact on alternative splicingRNASeptember 1, 2021Yongjun Chu, Shinnichi Yokota, Jing Liu, Audrius Kilikevicius, Krystal C. Johnson, David R. Coreyprimary antibodies in blocking buffer at 4 °C on rocking platform overnight: using anti-AGO1, 1:2000 (5053, Cell Signaling), antiAGO2, 1:1500 (015-22031, Fujifilm WAKO), anti-AGO3, 1:500 (39787, Active Motif),

(18) miRNA-mediated loss of m6A increases nascent translation in glioblastomaPLoS geneticsMarch 1, 2021Zepecki JP, Karambizi D, Fajardo JE, Snyder KM, Guetta-Terrier C, Tang OY, Chen JS, Sarkar A, Fiser A, Toms SA, et al.primary antibodies were used for Western blotting following immunoprecipitation: Ago1 (Cell Signaling, 5053S) and ILF3 (ProteinTech, 19887-1-AP). RNase FTO-AGOI RNA co-IP Glioma cells were seeded on tissue-culture

(19) Repressing Ago2 mRNA translation by Trim71 maintains pluripotency through inhibiting let-7 microRNAseLifeFebruary 18, 2021Liu Q, Chen X, Novak MK, Zhang S, Hu W.A5032 WB (1:5000) Antibody Rabbit monoclonal anti-Ago1 (D84G10) Cell Signaling Technology Cat# 5053 WB (1:1000) Antibody Rabbit monoclonal anti-Ago2 (C34C6) Cell Signaling Technology Cat# 2897 WB

(20) Peripherally misfolded proteins exacerbate ischemic stroke-induced neuroinflammation and brain injuryJournal of neuroinflammationJanuary 20, 2021Liu Y, Subedi K, Baride A, Romanova S, Callegari E, Huber CC, Wang X, Wang H.anti-CryAB (1:1000, Abcam; #ab13496), anti-Argonaute 1 (Argo1) (1:1000, Cell Signaling Technology, #5053, Lot 1), anti-Tsg101 (1:1000, Santa Cruz Biotechnology, #sc7964, Lot F0217), anti-ubiquitin (Ub) (1:1000,

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Argonaute 1 (D84G10) XP ® Rabbit mAb

Argonaute 1 (D84G10) XP ® Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (45)

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Argonaute 1 (D84G10) XP ® Rabbit mAb from Cell Signaling Technology

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