Fig 1: Effects of radiation on cancer-associated fibroblast (CAF) surface expression of checkpoint molecules. Surface expression of checkpoint ligands PD-L1, CD155, CD112, and HLA-E (MHC-I) were analyzed by flow cytometry on control and irradiated CAFs (iCAFs). Results are expressed as fold non-irradiated CAF median fluorescence intensity (MFI). Bars represent mean (± SD) values from two different CAF donors. Statistical P-values between control CAFs and irradiated CAF-groups were determined using two-way ANOVA with Dunnett correction for multiple comparisons. **P < 0.01 and ***P < 0.001.