RAT ANTI MOUSE CD206 MCA2235 from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemRAT ANTI MOUSE CD206
CompanyBio-Rad
Price $408.00Supplier Page View Company Product Page
Catalog NumberMCA2235
CloneMR5D3
NCBI Gene AliasesCD206, MR
TargetCD206
Quantity0.25 mg
ApplicationsFlow Cytometry,Immunohistology - Frozen,Immunoprecipitation,Immunofluorescence
FormatPurified IgG - liquid
IsotypeIgG2a
HostRat
ReactivityMouse
ClonalityMonoclonal Antibody
NCBI Full Gene Namemannose receptor, C type 1
ConcentrationIgG concentration 1.0 mg/ml

Questions and Answers

Can this antibody be used to study macrophage polarization in my experimental models?

Yes, the rat anti-mouse CD206 antibody (MCA2235) has been utilized in various studies to investigate macrophage polarization, particularly in the context of M2 macrophage markers. For instance, it was used in research examining the role of macrophages in atherosclerosis regression, where CD206 expression was associated with alternatively activated macrophages (Sharma et al., 2020).

Is this antibody effective for immunofluorescence applications?

The rat anti-mouse CD206 antibody has been shown to work effectively in immunofluorescence applications. In one study, it was employed at a dilution of 1:300 to visualize CD206 in tissue sections, demonstrating its utility in detecting macrophage populations in various experimental settings (Chen et al., 2023).

What specific role does CD206 play in macrophage function that might be relevant to my research?

CD206, also known as the mannose receptor, is involved in the recognition and phagocytosis of glycoproteins and plays a crucial role in mediating the immune response. It is particularly important in the context of alternative macrophage activation, which is associated with tissue repair and anti-inflammatory responses, making it a relevant marker for studies focused on macrophage function in various disease models (Welc et al., 2020).

Are there any specific experimental models where this antibody has been cited?

Yes, the CD206 antibody has been cited in multiple studies involving various experimental models, including those investigating macrophage behavior in renal fibrosis (Jiao et al., 2021) and vascular mimicry in tumors (Barnett et al., 2016). These applications highlight its versatility in studying macrophage-related processes across different biological contexts.

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injuryJournal of Clinical InvestigationApril 15, 2026Miao Wang, Zhentai Huang, Zhihong Du, Jiajing Shan, Qing Ye, Lingxiao Lu, Ming Jiang, Fei Xu, Ziyang Liu, David J.R. Fulton, et al.(Santa Cruz Biotechnology, sc-32790), rabbit anti-NEUN (Sigma-Aldrich, ABN78), rat anti-CD206 (Bio-Rad, MCA2235), and rabbit anti-MAP2 (Sigma-Aldrich, AB5622). For flow cytometry, we used CD11b-BUV737 (BD Biosciences,

(2) Sex-specific APOE4-dependent innate immunity regulates meningeal lymphatics, brain lipids, neuroinflammation, and cognitionNeuronMarch 26, 2026Nickoleta Delivanoglou, Kennedi T. Todd, Francisco Almeida, Shanon Rego, Amogh Changavi, Myriam Spajer, Virginia Estades Ayuso, Liliana M. Pinho-Correia, Guadalupe Sanchez, Sofia P. das Neves, et al.anti-mouse Podocalyxin,R&D Systems,Cat#AF1556 "Rat anti-mouse CD206, clone MR5D3",Bio-Rad Laboratories,Cat#MCA2235 Rabbit anti-mouse hAPOE,Cell Signaling Technology,Cat#13366S Goat anti-mouse IBA1,Abcam,Cat#ab5076 Rat

(3) Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelinationiScienceFebruary 20, 2026Weixing Yan, Dong Xiang, Li Du, Di Zhu, Qi Jia, Yuting Liu, Siyu Wang, Li Liu, Haihao Guan, Yelin Zhao, et al.mouse anti-Iba-1 antibody (1:400, Abcam, Cat: ab283319), rat anti-CD206 antibody (1:200, Bio-Rad, Cat: MCA2235), rat anti-BrdU antibody (1:400, Abcam, Cat: ab6326), mouse anti-phospho-Histone H3 (Ser10) antibody

(4) Th17 effector cytokines induce shared and distinct microglial and endothelial cell responses in post-streptococcal encephalitisbioRxivFebruary 7, 2026Charlotte R. Wayne, Ugur Akcan, Travis E. Faust, Violetta Duran-Laforet, Danny Jamoul, Luca Bremner, Nicole Ampatey, Büşra T. Akcan, Sarah J. Ho, Bogoljub Ciric, et al.,Synaptic Systems,234009,1:400 Iba1,Abcam,AB5076,1:500 Ifitm3,Proteintech,11714-1-AP,1:1000 CD74,BD Biosciences,151002,1:300 CD206,Bio-Rad,MCA2235,1:200 Mfsd2a,Cell Signalling,80302,1:1000 CD68,Abcam,AB53444,1:500 ZO-1,Invitrogen,61-7300,1:100 Claudin-5,Invitrogen,35-2500,1:100

(5) Functional border-associated macrophages limit Alzheimer's Disease progressionbioRxivFebruary 3, 2026Drew Adler, Natália Pinheiro-Rosa, Alon Millet, Zoran Z Gajic, Evan Cheng, Begoña Gamallo-Lana, Yosuke Morizawa, Eric Klann, Wen-Biao Gan, Adam C Mar, et al.019–19741, Wako), Guinea Pig anti-NeuN (1:1000, 266 004, Synaptic systems), Rat anti-CD206 (1:500, MCA2235, Bio-Rad), Rat anti LAMP1 (1:250, 1D4B, DSHB), Rabbit anti-Laminin (1:300, Thermo Fisher, PA116730).

(6) Vascular endothelial-specific loss of TGF-beta signaling as a model for choroidal neovascularization and central nervous system vascular inflammationeLifeDecember 23, 2025Yanshu Wang, Amir Rattner, Zhongming Li, Philip M Smallwood, Jeremy Nathans(Invitrogen 14-0542-82); rat mAb anti-mouse F4/80 (Bio-Rad MCA497G); rat mAb anti-mouse CD206 (Bio-Rad MCA2235); rat mAb anti-mouse PU.1/Spi-1 (R&D Systems MAB7124); mouse mAb anti-alpha SMA, Cy3 conjugate (Sigma-Aldrich

(7) Microglial colonization of the developing mouse brain is controlled by both microglial and neural CSF-1The EMBO JournalNovember 17, 2025Cécile Bridlance, Sarah Viguier, Nicolas Olivié, Edmond Dupont, Dorine Thobois, Benjamin Mathieu, Jean X Jiang, Guillermina López-Bendito, Melanie Greter, Burkhard Becher, et al.solution with the following primary antibodies overnight at 4 °C: rat anti-CD206 (1/200; Bio-Rad Cat# MCA2235, RRID:AB_324622), chicken anti-GFP (1/1000; Aves Labs Cat# GFP-1020, RRID:AB_10000240), rabbit anti-IBA1

(8) Microglia-neuron crosstalk through Hex-GM2-MGL2 maintains brain homeostasisNatureOctober 1, 2025Frosch M, Shimizu T, Wogram E, Amann L, Gruber L, Groisman AI, Fliegauf M, Schwabenland M, Chhatbar C, Zechel S, et al.overnight at a dilution of 1:1,000 for IBA1 (ab178846, Abcam; 234 308, Synaptic Systems), 1:500 for CD206 (MCA2235, Bio-Rad), 1:200 for collagen IV (AB769, Millipore), 1:500 for SOX9 (AF3075, R&D), 1:500 for NeuN (ab104224,

(9) Microglial cyclooxygenase-1 modulates cerebral capillary basal tone in vivo in miceNature communicationsJuly 1, 2025Mills WA 3rd, Savory NA, Lopez-Ortiz AO, Lentferink DH, González Ibáñez F, Agochi P, Rastegar E, Gupta A, Gupta D, Suram A, et al.0.1% Triton X 100 in 1× PBS, with each wash lasting 10 min. Primary antibodies CD206 (BioRad Cat # MCA2235, Lot # 148572) and α-smooth muscle actin (D4k9N) (Cell Signaling Technology Cat. No. 19245 Lot 6) were

(10) Chemogenetic activation of microglial Gi signaling decreases microglial surveillance and impairs neuronal synchronizationSci AdvFebruary 28, 2025Shunyi Zhao, Lingxiao Wang, Dimitrios Kleidonas, Fangfang Qi, Yue Liang, Jiaying Zheng, Anthony D. Umpierre, Long-Jun Wu(1:1000, Aves Labs, GFP-1010), rat anti-P2Y12 (1:100, BioLegend, 848002), rat anti-CD206 (1:250, Bio-Rad, MCA2235), mouse anti-NeuN (1:500, Abcam, Ab104224), mouse anti-GFAP (1:500, Cell Signaling Technology, 3670S),

(11) A clinically relevant model and method to study necrosis as a driving force in glioma restructuring and progressionProceedings of the National Academy of SciencesFebruary 18, 2025Jiabo Li, Ling-Kai Shih, Steven M. Markwell, Cheryl L. Olson, David P. Sullivan, Constadina Arvanitis, James L. Ross, Nicolas G. Lam, Hannah Nuszen, Dolores Hambardzumyan, et al.1:250, IBA1-0020, Ave Labs; mouse anti-HIF1α, 1:400, 610958, BD Biosciences; rat anti-CD206, 1:100, MCA2235, Bio-Rad; mouse anti-CD133, 1:50, HB#7, DSHC; rabbit anti-CXCR4, 1:250, ab181020, Abcam; rat anti-CD44,

(12) Myofibroblast-derived exosomes enhance macrophages to myofibroblasts transition and kidney fibrosisRenal FailureDecember 31, 2024Wenqiang Yu, Jinfang Song, Shuangquan Chen, Jiayi Nie, Chujun Zhou, Jiamin Huang, Hua LiangNJ), anti-F4/80 antibody (1:100, No. MCA497GA, Bio-Rad, Hercules, CA), anti-CD206 antibody (1:100, No. MCA2235, Bio-Rad, Hercules, CA), or anti-αSMA antibody (1:100, No. ab5694, Abcam, Waltham, MA). The immunofluorescence

(13) Early intermittent hyperlipidaemia alters tissue macrophages to fuel atherosclerosisNatureOctober 10, 2024Minoru Takaoka, Xiaohui Zhao, Hwee Ying Lim, Costan G. Magnussen, Owen Ang, Nadine Suffee, Patricia R. Schrank, Wei Siong Ong, Dimitrios Tsiantoulas, Felix Sommer, et al.MCA519G; 1:200), anti-CD68 (rat monoclonal, Bio-Rad, MCA1957, 1:300), anti-CD206 (rat monoclonal, Bio-Rad, MCA2235, 1:200), anti-CD3 (rabbit polyclonal, DAKO/Agilent, A045229-2, 1:200), anti-LYVE1 (rabbit monoclonal,

(14) IRF8 and MAFB drive distinct transcriptional machineries in different resident macrophages of the central nervous systemCommunications biologyJuly 24, 2024Yamasaki A, Imanishi I, Tanaka K, Ohkawa Y, Tsuda M, Masuda T.primary antibody for Iba1 (1:1000 018-28523, FUJIFILM Wako; 1:1000 234 308, Synaptic Systems), CD206 (MCA2235, Biorad), Collagen IV (1:200 AB769, Millipore), CD11b (1:1000 MCA711G, Biorad) and AXL (1:500 AF854,

(15) An inducible genetic tool to track and manipulate specific microglial states reveals their plasticity and roles in remyelinationImmunityJune 1, 2024Kia M. Barclay, Nora Abduljawad, Zuolin Cheng, Min Woo Kim, Lu Zhou, Jin Yang, Justin Rustenhoven, Jose Mazzitelli Mazzitelli, Leon C. D. Smyth, Dvita Kapadia, et al.anti-mDECTIN-1-IgG (clone R1–4E4) (Invivogen, mabg-mdect, 1:30); Rat monoclonal anti-CD206 (clone MR5D3) (BioRad, MCA2235); Armenian hamster monoclonal anti-CD31 (clone 2H8) (Invitrogen, MA3105); Rabbit anti-Olig2 (EMD Milipore

(16) CCR2+ monocytes replenish border-associated macrophages in the diseased mouse brainCell ReportsApril 23, 2024Lingxiao Wang, Jiaying Zheng, Shunyi Zhao, Yushan Wan, Meijie Wang, Dale B. Bosco, Chia-Yi Kuan, Jason R. Richardson, Long-Jun Wutemperature for 1 h. Sections were then incubated with primary antibodies (Rat anti Mouse CD206, BIO-RAD, MCA2235, 1:100; Goat anti Mouse CD206, R&D, AF2535, 1:500; Goat anti Human DTR, R&D, AF-259-NA, 1:300; Rat anti Mouse CD31

(17) A comparative evaluation of the strengths and potential caveats of the microglial inducible CreER mouse modelsCell ReportsJanuary 23, 2024Alicia M. Bedolla, Gabriel L. McKinsey, Kierra Ware, Nicolas Santander, Thomas D. Arnold, Yu Luo,Cell Signaling,Cat#4604s "LYVE1 (1:600, host: rabbit)",Abcam,Cat#ab14917; RRID: AB_301509 "CD206 (1:200, host: rat)",Biorad,Cat#mca2235; RRID: AB_324622 "Donkey anti-Goat IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™

(18) Myeloid cell-derived creatine in the hypoxic niche promotes glioblastoma growthCell metabolismJanuary 2, 2024Rashidi A, Billingham LK, Zolp A, Chia TY, Silvers C, Katz JL, Park CH, Delay S, Boland L, Geng Y, et al.methodology. Samples were stained with primary antibodies for Iba1 (IBA1-0020, Aves, macrophages) and CD206 (MCA2235, BioRad, M2-like macrophages) then counterstained with DAPI (nuclei). Macrophage markers were visualized

(19) An inducible genetic tool for tracking and manipulating specific microglial states in development and diseasebioRxivDecember 4, 2023Kia M. Barclay, Nora Abduljawad, Zuolin Cheng, Min Woo Kim, Lu Zhou, Jin Yang, Justin Rustenhoven, Jose Mazzitelli Mazzitelli Perez, Leon Smyth, Wandy Beatty, et al.anti-mDECTIN-1-IgG (clone R1–4E4) (Invivogen mabg-mdect, 1:30); Rat monoclonal anti-CD206 (clone MR5D3) (BioRad, MCA2235); Armenian hamster monoclonal anti-CD31 (clone 2H8) (Invitrogen, MA3105); Rabbit anti-Olig2 (EMD Milipore

(20) Depletion of perivascular macrophages delays ALS disease progression by ameliorating blood-spinal cord barrier impairment in SOD1G93A miceFrontiers in Cellular NeuroscienceNovember 24, 2023Kazuki Adachi, Kota Miyata, Yukino Chida, Mikako Hirose, Yuta Morisaki, Koji Yamanaka, Hidemi MisawaBirmingham, AL, USA). The primary antibodies and dilutions used were as follows: rat anti-CD206 (1:200, MCA2235, Bio-Rad, Hercules, CA, USA), goat anti-CD31 (1:300, AF3628, R&D Systems, Minneapolis, MN, USA), rabbit

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RAT ANTI MOUSE CD206

RAT ANTI MOUSE CD206

RAT ANTI MOUSE CD206

RAT ANTI MOUSE CD206

RAT ANTI MOUSE CD206

RAT ANTI MOUSE CD206 from Bio-Rad (Formerly AbD Serotec)

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RAT ANTI MOUSE CD206 from Bio-Rad (Formerly AbD Serotec)

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