Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb 52873S from Cell Signaling Technology

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Frontiers in oncology
January 01 2021

Fig 1: KLK8 decreased protein levels of pro-apoptotic proteins through the activation of PI3K-Akt-mTOR pathway. Mia-paca-2 and Panc-1 cells in Lv-KLK8 (A) and Lv-Control (B) were treated with PI3K inhibitor LY294002(75 μM), Akt inhibitor Deguelin (500 nM), mTOR inhibitor Rapamycin (100 nM) or Notch inhibitor RO4929097 (10 μM). (A, B) protein levels of pro-apoptotic markers cleaved caspase-3, cleaved caspase-9 and Bax were assessed by western blot.
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Molecular Medicine Reports
May 16 2019

Fig 2: miR-30a-5p overexpression promoted the proliferation and inhibited the apoptosis of HPAECs under hypoxic conditions for 48 h. (A) The level of miR-30a-5p was significantly increased in HPAECs transfected with miR-30a-5p mimics ***P<0.001 vs. NC. (B) Effect of miR-30a-5p overexpression on the proliferation of HPAECs under hypoxic conditions. *P<0.05 vs. NC. (C) Effect of miR-30a-5p overexpression on the apoptosis of HPAECs under hypoxic conditions. ***P<0.001 vs. NC. (D) Effect of miR-30a-5p overexpression on the level of cleaved caspase-3, cleaved caspase-9, Bax and Bcl-2 of HPAECs under hypoxia. The left panel includes a representative western blot analysis gel. The lower panel includes the densitometric analysis result of relative protein expression referenced to GAPDH. *P<0.05 vs. NC. miR, microRNA; HPAECs, human pulmonary artery endothelial cells; NC, negative control; OD, optical density; Bax, Bcl-2- associated X protein.
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Journal of Cancer
January 01 0001

Fig 3: Knockdown of PRPS2 induced prostate cancer cell apoptosis and cell cycle arrest. (A) PRPS2 knockdown induced PCa cell cycle arrested in G0/G1 phase. (B) Representative micrographs (left) and quantification (right) of EdU incorporation assay. **P < 0.01, ***P < 0.001. (C) PRPS2 knockdown increased apoptosis rate. *P < 0.05. (D) Western blot showed the protein levels of cell apoptosis-related proteins (cleavage of caspase-3, caspase-9, PARP, Bcl-2, Bax and p53) and cell cycle-related proteins (p27, CDK4 and cyclin D1) after down-regulated expression of PRPS2 in PC-3 (left) and DU145(right) cells. β-actin was served as loading control. NC, negative control.
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Nature Communications
December 16 2021

Fig 4: Engineered cell lines establish hRpn13 requirement for XL5-PROTAC-induced apoptosis.a Chemical structures of XL5 (orange)-PROTACs (VHL, blue; CRBN, green, IAP, burgundy). b RPMI 8226 cells were treated with the indicated concentration of XL5 (orange), XL5-VHL (navy), XL5-VHL-2 (blue), XL5-CRBN (green), XL5-IAP (burgundy), VHL-ligand (light blue), thalidomide (light green), or IAP-ligand (pink) for 48 h and cell metabolism measured by an MTT assay; data represent mean ± SD of n = 6 biological replicates. Viability is plotted as (λ570)sample/(λ570)control*100 (%). IC50 values are listed for XL5 and XL5-PROTAC. c Immunoblot of whole-cell extract from RPMI 8226 WT, trRpn13-MM1, or trRpn13-MM2 cells probing hRpn13 (1 s and 20 min exposure) or β-actin. d Sanger sequencing analyses of hRpn13 cDNA from RPMI 8226 WT, trRpn13-MM1, or trRpn13-MM2 cells denoting the location of the two sgRNAs (red arrow) on hRpn13-encoding gene ADRM1 Exon 2 with cDNA sequence (CDS) labeled. Allele is abbreviated as “A”. e RPMI 8226 WT (blue), trRpn13-MM1 (black) or trRpn13-MM2 (gray) cells were treated with the indicated concentrations of XL5-VHL-2 for 48 h and cell metabolism measured by an MTT assay; data represent mean ± SD of n = 6 biological replicates. Viability is calculated as (λ570)sample/(λ570)control*100 (%). f Immunoblots of whole cell lysate from RPMI 8226 WT, trRpn13-MM1, or trRpn13-MM2 cells treated for 24 h with 40 μM XL5-VHL-2 with comparison to DMSO (vehicle control) immunoprobing for cleaved caspase-9 (top panel), hRpn13 (two middle panels with 1 min or 10 min exposure), or β-actin (as a loading control, bottom panel). A black asterisk indicates cleaved caspase-9 in the 1-min immunoblot for hRpn13, as hRpn13 was probed following cleaved caspase-9 and without stripping the membrane.
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Clinical and translational medicine
June 01 2022

Fig 5: Overexpression of YTHDF2 desensitises ICC cells to cisplatin. (A) Representative images and the IHC scores for YTHDF2 in 12 chemoresisitant and 12 chemosensitive ICC tissues. (B) Cell viability of shControl or shYTHDF2‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (20 or 10 μM, respectively). (C) Representative flow cytometry analysis and mean fluorescent intensity of γ‐H2AX in shControl or shYTHDF2‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (20 or 10 μM, respectively). (D) Apoptosis analysis of shControl or shYTHDF2‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (60 or 40 μM, respectively). (E) Immunoblotting to measure caspase‐9, cleaved caspase‐9, caspase‐3, cleaved caspase‐3 protein levels in shControl or shYTHDF2‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (60 or 40 μM, respectively). (F) Cell viability of control or YTHDF2 vector‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (10 or 5 μM, respectively). (G) Representative flow cytometry analysis and mean fluorescent intensity of γ‐H2AX in control or YTHDF2 vector‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (20 or 10 μM, respectively). (H) Apoptosis analysis of control or YTHDF2 vector‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (60 or 40 μM, respectively). (I) Immunoblotting to measure caspase‐9, cleaved caspase‐9, caspase‐3, cleaved caspase‐3 protein levels in control or YTHDF2 vector‐transfected HuCC‐T1 and HCCC‐9810 cells after treatment with cisplatin (60 or 40 μM, respectively). The data are presented as mean ± SD and compared by t‐test. *p < .05; **p < .01; *** p < .001

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Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb

The Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody to CASP9, and Caspase 9. This antibody recognizes Homo Sapiens, and Human antigen. The Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb Antibody has been shown to work in the following applications: Immunocytochemistry, Immunofluorescence, and Western Blot.

Description

Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb recognizes endogenous levels of caspase-9 protein only when cleaved at Asp330

Product Specs
ItemCleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb
CompanyCell Signaling Technology
Price $401.00 Inquire
Catalog Number52873S
Quantity100 µl
ApplicationsW, IF-IC
ReactivityH
TargetCasp9 (Asp330, 17 kD) cleaved
HostRabbit
NCBI Full Gene Namecaspase 9
NCBI Gene AliasesAPAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
ClonalityMonoclonal
Research AreaApoptosis
Molecular Weight37

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Phone: 978-867-2388
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Website: http://www.cellsignal.com/

Website: Technical Support

(1) A cell death program–based tumor signature stratifies prognosis, immune landscape, and therapeutic response in gliomaFrontiers in OncologyMay 21, 2026Huaichao Zhang, Zijiang Yang, Qiang Xie, Pin Chen, Jinlong Huang, Shuang Liu, Zeyang Li, Wei Sun, Xiaobiao Zhang, Tao Xieanti-cleaved-Caspase-3 (Asp175), #9664, Cell Signaling Technology, Boston, USA; anti-cleaved-Caspase-9 (Asp330), #52873, Cell Signaling Technology, Boston, USA; anti-cleaved-Caspase-8 (Asp387), #8592, Cell Signaling Technology,

(2) Selective elimination of myeloid HIV reservoirs by targeting pro-survival pathwaysJournal of InfectionMay 1, 2026Min Li, Baichao Sun, Laurie J. Minze, Edward A. Graviss, Matthew Vasquez, Hong Zhao, Benjamin B. Gelman, Min Chen, Jin WangLaboratories) and CASPASE-7 (1:1000, M053–3, Medical & Biological Laboratories), cleaved CASPASE-9 (1:1000, 52873s, Cell Signaling), CASPASE-3 (1:1000, 9665s, Cell Signaling), cleaved CASPASE-3 (1:1000, 9501s, Cell Signaling

(3) Nuclear translocation of SLC25A10 isoform 3 promotes chemoresistance in HCC cells via CEBPB/BCL2A1 signalingCell Death & DiseaseApril 9, 2026Dan Liu, Shuang Dong, Siwei Cheng, Yuanyuan Lin, Sheng Chen, Jiaxin Gao, Bo Bi, Na Li, Jun Mi, Wujun XiongCaspase-3 (Cat # 9664, CST, MA, USA), Caspase-9 (Cat # 9508, CST, MA, USA), Cleaved Caspase-9 (Cat # 52873, CST, MA, USA), CEBPB (Cat # PA5-27244, Invitrogen, CA, USA), Cytochrome c (Cat # 4272, CST, MA, USA),

(4) Edelfosine induces cell cycle arrest and apoptosis in vascular smooth muscle cells to suppress neointimal hyperplasiaScientific ReportsMarch 25, 2026Jiaxing Sun, Yu Gui, Yuxin Liu, Yanan Guo, Rosana González Granado, Liam Guetg, Warren Peng, Shenghua Zhou, Xi-Long ZhengGAPDH (#5174), caspase-7 (#12827), cleaved caspase-7 (#8438), caspase-9 (#9508), cleaved caspase-9 (#52873), Bax (#2772), eIF2α (#9722), phospho-eIF2α (#9721), and goat anti-rabbit secondary antibody (#7074P2)

(5) Synaptotagmin-7 drives stress-induced cardiomyocyte necroptosis via the p53-Bak-mPTP axisTheranosticsJanuary 1, 2026Jialei Li, Shuang Wang, Yu Han, Jinhong Liu, Yuhan Li, Jingyu Zhang, Liping Guo, Yue Jin, Jiasong Chang, Ting Liu, et al.anti-Bak (CST, USA, #12105), anti-Cleaved Caspase 3 (CST, USA, #9664), anti-Cleaved Caspase 9 (CST, USA, #52873), anti-Cleaved Caspase 7 (CST, USA, #8438), anti-Bax (CST, USA, #2772), anti-Bim (CST, USA, #2933),

(6) Excavatolide C has oxidative-stress-dependent antiproliferative and apoptotic effects against breast cancer cellsBMC CancerJuly 1, 2025Jun-Ping Shiau, Che-Wei Yang, Wangta Liu, Szu-Yin Yu, Chia-Hung Yen, Fang-Rong Chang, Jyh-Horng Sheu, Hsueh-Wei Changcleaved caspase-8 (Asp374) (18C8), and cleaved caspase-9 (Asp330) (E5Z7N) rabbit mAb (#9664, #9496, and #52873) (Cell Signaling Technology, Danvers, MA, USA), were used to detect the activation (cleavage) of caspases

(7) C1QL1 inhibits breast cancer through the HSP90α/VCP-ERS/UPR axisExperimental & molecular medicineJune 1, 2025Zhang N, Shao Q, Xiang X, Yan C, Tao D, Li Q, Rong H, Zhao Y, Xiang T, Zeng X.CST), anti-Bax (2772, CST), anti-Bcl-2 (4223, CST), anti-Caspase 9 (9508, CST), anti-cleaved Caspase 9 (52873, CST), anti-Caspase 7 (12827, CST), anti-cleaved Caspase 7 (8438, CST), anti-Caspase 3 (14220, CST),

(8) Evernic Acid: A Low-Toxic and Selective Alternative to Chemotherapeutic Agents in the Treatment of Ovarian CancerArch Pharm (Weinheim)May 22, 2025Mine Ensoy, Damla Nur Parıltı, Ayşe Hale Alkan, Kübra Nur Kaplan İlhan, Pelin Mutlu, Bala Gür Dedeoğlu, Demet Cansaran-Duman(1:1,000, CST, Cat#9664), Caspase 9 (1:1,000, CST, Cat# 9508), Cleaved Caspase‐9 (1:1,000, CST, Cat#52873), Survivin (BIRC5) (1:1000, Santa Cruz, Cat#sc‐17779), and β‐Actin (1:1,000, St. John's, Cat# STJ91464).

(9) The Molecular Chaperone TCP1 Affects Carcinogenicity and Is a Potential Therapeutic Target for Acute Myeloid LeukemiaPharmaceuticsApril 24, 2025Yong Wu, Guihui Tu, Yuxia Yuan, Jingwen Liu, Qingna Jiang, Yang Liu, Qiurong Wu, Lixian Wu, Yuanzhong Chenanti-GAPDH monoclonal antibody (ImmunoWay, YM3029); anti-AML1-ETO (CST, 4334); anti-cleaved-caspase-9 (CST, 52873); anti-cleaved-PARP (CST, 5625); anti-PLK1 (Abcam, ab189139); anti-pan-myc (Abcam, Ab195207); anti-mouse

(10) Optimization of the PROTAC linker region of the proteasome substrate receptor hRpn13 rationalized by structural modeling with molecular dynamicsJournal of Biological ChemistryApril 19, 2025Xiuxiu Lu, Venkata R. Sabbasani, Bakar Hassan, Rolf E. Swenson, Kylie J. Walters(Cell Signaling Technology 3700s, 1:6000 or 1:10,000), anti-cleaved caspase-9 (Cell Signaling Technology, 52873s, 1:500), anti-caspase-3 (Cell Signaling Technology, 9662s, 1:1000), and secondary antibodies anti-mouse

(11) Chemical Tools Based on the Tetrapeptide Sequence of IL-18 Reveals Shared Specificities between Inflammatory and Apoptotic Initiator CaspasesbioRxiv : the preprint server for biologyFebruary 27, 2025Bourne CM, Raniszewski NR, Kulkarni M, Exconde PM, Liu S, Yost W, Wrong TJ, Patio RC, Mahale A, Kardhashi M, et al.(mCasp1),Adipogen,AG-20B-0042-C100 Recombinant-Anti-GSDMD (mGSDMD),Abcam,ab209845 Cleaved Caspase-9 (Asp330),Cell Signaling,52873S IRDye 800CW anti-rabbit,LI-COR,Cat#926-32213; RRID:AB_621848 IRDye 800CW anti-mouse,LI-COR,Cat#926-32212;

(12) Transcriptional responses to direct and indirect TGFB1 stimulation in cancerous and noncancerous mammary epithelial cellsCell communication and signaling : CCSOctober 28, 2024Janus P, Kuś P, Jaksik R, Vydra N, Toma-Jonik A, Gramatyka M, Kurpas M, Kimmel M, Widłak W.PARP1 (1:1,000, #5625, CST), cleaved Caspase-7 (1:1,000, #8438, CST), cleaved Caspase-9 (1:1,000, #52873, CST), VIM (1:1,000, #5741, CST), ESR1/ERα (1:2,000, #8644, CST), HSPA8/HSC70 (1:5,000, #sc-7298, Santa Cruz Biotechnology

(13) Investigation of cannabidiol-induced cytotoxicity in human hepatic cellsToxicologyAugust 1, 2024Si Chen, Xilin Li, Qiangen Wu, Yuxi Li, Montserrat Puig, Frederic Moulin, Supratim Choudhuri, Jeremy Gingrich, Lei Guoto probe CDK2 (# 2546), CDK6 (# 3136), cyclin D1 (# 2978), cyclin D3 (# 2936), cleaved-caspase 9 (# 52873), cleaved-caspase 8 (# 9496), cleaved-caspase 3 (# 9664), Bcl-2 (# 15071), γ-H2A.X (# 2577), PARP (#

(14) Combined treatment with cetuximab and STA9090 has synergistic anticancer effects on human non-small cell lung cancerActa Biochimica et Biophysica SinicaJuly 1, 2024Wanjun Lu, Lixia Liu, Xiang Kang, Kangkang Ren, Ye Huang, Minzhang Cheng, Xiaolei Li, Fei Xu, Xinping XuAKT (4691s), PARP (9542s), cleaved-PARP D214 (5625s), caspase-9 (9508s), and cleaved-caspase9 D330 (52873s). Antibodies directed against phosphorylation-specific (Ser473) Akt and phosphorylation-specific (Thr202/Tyr204)

(15) Taenia solium cysticerci's extracellular vesicles Attenuate the AKT/mTORC1 pathway for Alleviating DSS‐induced colitis in a murine modelJournal of Extracellular VesiclesMay 23, 2024Suraj Singh Rawat, Anand Kumar Keshri, Naina Arora, Rimanpreet Kaur, Amit Mishra, Rajiv Kumar, Amit PrasadD1Q5S), LC3 (CST, 4108), β‐Actin (CST, D6A8), Cleaved Caspase‐3 (CST, 9661S), Cleaved Caspase‐9, (CST, 52873S), Goat Anti‐Mouse IgG (BIO‐RAD, 1706516) and IP6K1 (Santa Cruz, sc‐374292), p‐TSC2 (CST, 3617S), TSC2

(16) A structure-based designed small molecule depletes hRpn13(Pru) and a select group of KEN box proteinsNature communicationsMarch 20, 2024Lu X, Chandravanshi M, Sabbasani VR, Gaikwad S, Hughitt VK, Gyabaah-Kessie N, Scroggins BT, Das S, Myint W, Clapp ME, et al.Signaling Technology 3700 s, 8H10D10, 1:6,000 or 1:10,000), anti-cleaved caspase-9 (Cell Signaling, 52873 s, E5Z7N, 1:500), anti-caspase-3 (Cell Signaling, 9662 s, 1:1000), anti-PCLAF (Santa Cruz, sc-390515 HRP

(17) Sleep fragmentation induces heart failure in a hypertrophic cardiomyopathy mouse model by altering redox metabolismiScienceMarch 15, 2024Bose K, Agrawal R, Sairam T, Mil J, Butler MP, Dhandapany PS.Cell Signaling Technology), Vdac (Cat No. 4661, Cell Signaling Technology), Cleaved caspase 9 (Cat No. 52873, Cell Signaling Technology), Cleaved caspase 3 (Cat No.9664, Cell Signaling Technology), Nrf2 (Cat No.

(18) Overcoming brain-derived therapeutic resistance in HER2+ breast cancer brain metastasisbioRxivFebruary 28, 2024Danyyl Ippolitov, Yi-Han Lin, Jeremy Spence, Aleksandra Glogowska, Thatchawan Thanasupawat, Jason Beiko, Marc R. Del Bigio, Xin Xu, Amy Wang, Raul Calvo, et al.20min; 1:100 in 5% NGS - 4C overnight,N/A Cleaved Caspase-9 (Asp330) (E5Z7N),Cell Signaling Technology,52873,AB_2799423,Rabbit,Monoclonal,H,1:1000 in 5% BSA - 4C overnight,N/A,N/A Cleaved PARP (Asp214) (D64E10),Cell Signaling Technology

(19) hRpn13 shapes the proteome and transcriptome through epigenetic factors HDAC8, PADI4, and transcription factor NF-κB p50Molecular CellFebruary 1, 2024Vasty Osei-Amponsa, Monika Chandravanshi, Xiuxiu Lu, Valentin Magidson, Sudipto Das, Thorkell Andresson, Marzena Dyba, Venkata R. Sabbasani, Rolf E. Swenson, Caroline Fromont, et al.,Cell Signaling Technology,Cat#4806; RRID: AB_2282911 Rabbit anti-Cleaved Caspase 9,Cell Signaling Technology,Cat#52873; RRID: AB_2799423 Rabbit anti-NF-κB p105/p50,Abcam,Cat#ab32360 Rabbit anti-TACSTD2,Cell Signaling Technology

(20) Carnosic acid mitigates doxorubicin-induced cardiac toxicity: Evidence from animal and cell model investigationsIran J Basic Med SciJanuary 1, 2024Mahboobeh Ghasemzadeh Rahbardar, Farhad Eisvand, Maryam Rameshrad, Bibi Marjan Razavi, Abbas Tabatabaee Yazdi, Hossein Hosseinzadehanti-caspase 8 (Cell Signaling #4790, 1:1000) 7. Rabbit monoclonal anti-caspase 9 (Cell Signaling #52873, 1:1000) After incubation with the primary antibodies, the blots were washed three times with TBST.

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Frontiers in oncology

Molecular Medicine Reports

Journal of Cancer

Nature Communications

Clinical and translational medicine

Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

Citations (60)

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Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb from Cell Signaling Technology

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