Fig 1: ERK1 governs immunomodulatory properties of MSCs via ETS2/AURKA/NF-κB signaling(A) NF-κB, p-NFκB, IKB, p-IKB, and p-IKK expression levels were detected by western blot analysis.(B) AURKA, p-AURKA, and ETS2 expression levels were detected by western blot analysis.(C) The relative levels of proteins were presented in column.(D) Promoter regions of aurka were examined by PROMO search tools, and ETS2-bound DNA sites were identified by ChIP assay.(E) aurka promoter activities were measured by luciferase assay, and relative light unit was presented in column.(F) Schema indicating of the ERK1/ETS2/AURKA/NF-κB/Fas cascade. Comparisons between means groups were analyzed using ordinary one-way ANOVA with Tukey’s multiple comparisons test. Bars show mean ± SEM. Statistical significance is indicated with ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.