Mouse/Rat Integrin ß1/CD29 Antibody AF2405 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemMouse/Rat Integrin ß1/CD29 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF2405
Quantity100 ug (also 25 ug)
ApplicationsFlow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Frozen,Simple Western,Immunofluorescence,Immunocytochemistry,CyTOF-ready
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
ConcentrationLYOPH
PurityAntigen Affinity-purified
TargetIntegrin beta 1/CD29
ReactivityHuman,Mouse,Rat,Transgenic Mouse
HostGoat
IsotypeIgG
Antibody TypePrimary Antibodies
NCBI Gene AliasesGm9863, CD29, Fnrb, 4633401G24Rik, gpIIa
NCBI Full Gene Nameintegrin beta 1 (fibronectin receptor beta)
ClonalityPolyclonal
ImmunogenChinese hamster ovary cell line CHO-derived recombinant mouse Integrin ß1, Gln21-Ala738, Accession # P09055
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used for immunofluorescence applications in my studies on mesenchymal stem cells?

Yes, the Integrin beta 1/CD29 antibody has been shown to work in immunofluorescence applications. For instance, in a study examining bone marrow-derived vascular precursor cells, the antibody was utilized to detect integrin beta 1 in fixed cells, indicating its suitability for immunofluorescence assays ().

What species does this antibody react with, and is it suitable for studies involving rat models?

The Integrin beta 1/CD29 antibody is reactive with both mouse and rat species. It has been successfully used in various applications, including Western blotting and immunohistochemistry, for both species, making it suitable for studies involving rat models ().

How does this antibody perform in flow cytometry applications?

The Integrin beta 1/CD29 antibody has been utilized in flow cytometry to analyze bone marrow-derived mesenchymal stem cells. In one study, it was employed to characterize cell surface antigens, demonstrating its effectiveness in flow cytometry applications ().

Is there any evidence of this antibody being used in vivo, particularly in wound healing studies?

Yes, the Integrin beta 1/CD29 antibody has been referenced in studies involving in vivo applications. For example, it was used to assess the role of bone marrow-derived vascular precursor cells in wound healing, highlighting its relevance in vivo ().

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) FOXF2 regulates pericyte–endothelial signaling required for vascular homeostasis after neonatal hyperoxic lung injuryNature CommunicationsFebruary 12, 2026Fei Sun, Yuchen Zhao, Jonathan Do, Peng Cheng, Enhong Li, Yanru Liu, Shawyon P. Shirazi, Jennifer M. S. Sucre, Tanya V. Kalin, Hua He, et al.°C with rabbit anti-ANGPTL4 (Thermo Fisher, 40-9800, 1:100) and goat anti-Integrin β1 (R&D Systems, AF2405, 1:200) antibodies diluted in the antibody diluent provided with the kit. After primary antibody incubation,

(2) PI(3)P coordinates SNX17- and SNX27-dependent protein recycling for long-term synaptic plasticityThe Journal of cell biologyNovember 3, 2025Rivero-Ríos P, Uygun T, Chavis GD, Lee H, Duan B, Sutton MA, Weisman LS.Abcam), Phospho-(Ser/Thr) rabbit pAb (1:1000, 9631, Cell Signaling), β1-integrin goat pAb (1:1,000, AF2405; R&D Systems), GluA1 rabbit pAb (1:1,000, ABN241, Millipore Sigma), and GAPDH rabbit mAb (1:2,000, 2118;

(3) Spatiotemporal dynamics exhibited by horizontal basal cells reveal a pro-neurogenic pathway during injury-induced olfactory epithelium regenerationiScienceMay 17, 2024Jonathan D. Louie, Camila M. Barrios-Camacho, Benjamin H. Bromberg, Constantin A. Hintschich, James E. SchwobHybridoma Bank,CPTC-Ezrin-1 Rabbit α-Laminin,Novus Biologicals,NB300-144 Goat α-Itgb1,R&D Systems,AF2405 Rat α-Itgb4,R&D Systems,MAB4054 Chicken α-CK5,BioLegend,Clone Poly9059 Rabbit α-Rac1,Proteintech,24072-1-AP Rabbit

(4) Tissue Inhibitor of Metalloproteinases-1 Overexpression Mediates Chemoresistance in Triple-Negative Breast Cancer CellsCellsJuly 7, 2023Agnello L, d'Argenio A, Caliendo A, Nilo R, Zannetti A, Fedele M, Camorani S, Cerchia L.

(5) Recruitment of the SNX17-Retriever recycling pathway regulates synaptic function and plasticityThe Journal of cell biologyJuly 3, 2023Rivero-Ríos P, Tsukahara T, Uygun T, Chen A, Chavis GD, Giridharan SSP, Iwase S, Sutton MA, Weisman LS.11938-1-AP; Proteintech), GFP rabbit mAb (1:1,000, Ab32146; Abcam), β1-integrin goat pAb (1:1,000, AF2405; R&D Systems), and GAPDH rabbit mAb (1:2,000, 2118; Cell Signaling). Following washes in TBS containing

(6) Mechanical tension mobilizes Lgr6⁺ epidermal stem cells to drive skin growthScience advancesApril 29, 2022Xue Y, Lyu C, Taylor A, Van Ee A, Kiemen A, Choi Y, Khavanian N, Henn D, Lee C, Hwang L, et al.1:400; dsRED; Mybioscience.com, MBS448092), anti-Integrin β1 (ITGB1), (goat; 10 μg/ml; R&D Systems, AF2405), anti-Tenascin C (TNC) (rat; 10 μg/ml; R&D Systems, MAB2138-SP), and anti-vimentin (mouse; 1:50; Santa Cruz Biotechnology

(7) Cdc42 activity in Sertoli cells is essential for maintenance of spermatogenesisCell reportsOctober 26, 2021Heinrich A, Bhandary B, Potter SJ, Ratner N, DeFalco T.

(8) RNF213 gene silencing upregulates transforming growth factor β1 expression in bone marrow-derived mesenchymal stem cells and is involved in the onset of Moyamoya diseaseExperimental and therapeutic medicineSeptember 1, 2021Wang C, Sun C, Zhao Y, Song H, Li Z, Jin F, Cui C.25-30 min with antibodies against the following cell surface antigens: CD29 (dilution, 1:50; cat. no. AF2405; R&D Systems), CD106 (dilution, 1:40; cat. no. ab134047; Abcam), CD34 (dilution, 1:50; cat. no. ab81289;

(9) SCA-1/Ly6A Mesodermal Skeletal Progenitor Subpopulations Reveal Differential Commitment of Early Limb Bud CellsFrontiers in cell and developmental biologyJanuary 1, 2021Marín-Llera JC, Lorda-Diez CI, Hurle JM, Chimal-Monroy J.X-100 in PBS and incubated with anti-CD29 (1:100, R&D Systems, Minneapolis, MN, United States, cat. no. AF2405), PE anti-mouse/human CD44 (1:300, Bio Legend, cat. no. 1030007), anti-CD73 (1:500, Abcam, Cambridge,

(10) Periostin and matrix stiffness combine to regulate myofibroblast differentiation and fibronectin synthesis during palatal healingMatrix biology : journal of the International Society for Matrix BiologyDecember 1, 2020Nikoloudaki G, Snider P, Simmons O, Conway SJ, Hamilton DW.Sigma-Aldrich), anti-fibronectin (ab23750, 1:100, Abcam), vinculin (MAB3574, Millipore), β1-integrin (AF2405, R&D Systems) which was detected with appropriate IgG conjugated to Alexa Fluor secondary antibodies

(11) Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound HealingCell stem cellSeptember 3, 2020Abbasi S, Sinha S, Labit E, Rosin NL, Yoon G, Rahmani W, Jaffer A, Sharma N, Hagner A, Shah P, et al.

(12) Modulation of the secretion of mesenchymal stem cell immunoregulatory factors by hydrolyzed fish collagenExperimental and therapeutic medicineJuly 1, 2020Liu C, Sun J.primary antibodies for the BMSC protein samples at room temperature for 1 h: Anti-CD29 (1:500; cat. no. AF2405, R&D Systems, Inc.), anti-CD90 (1:1,000; cat. no. ab92574; Abcam), anti-CD34 (1:1,000 dilution; cat.

(13) Distinct Roles for Rac1 in Sertoli Cell Function during Testicular Development and SpermatogenesisCell reportsApril 14, 2020Heinrich A, Potter SJ, Guo L, Ratner N, DeFalco T.

(14) The spatiotemporal expression patterns of MSC-associated markers contribute to the identification of progenitor subpopulations in developing limbsThe International journal of developmental biologyJanuary 1, 2020García-Cervera AS, Chimal-Monroy J, Marín-Llera JC.Subsequently, the primary antibodies were used against CD29 (1:100; R&D Systems, Minneapolis, MN, cat. no. AF2405), CD44-PE (1:300; Biolegend, San Diego, CA, cat. no. 400607), CD105 (1:100; R&D Systems, cat. no. AF1320),

(15) Proteomics reveals Rictor as a noncanonical TGF-β signaling target during aneurysm progression in Marfan miceAmerican journal of physiology. Heart and circulatory physiologyNovember 1, 2018Parker SJ, Stotland A, MacFarlane E, Wilson N, Orosco A, Venkatraman V, Madrid K, Gottlieb R, Dietz HC, Van Eyk JE.

(16) Endothelial semaphorin 7A promotes seawater aspiration-induced acute lung injury through plexin C1 and β1 integrinMolecular medicine reportsOctober 1, 2017Zhang M, Yan X, Liu W, Sun R, Xie Y, Jin F.obtained from R&D Systems, Inc. Antibody for plexin C1 (cat. no. AF5375) and β1 integrin (cat. no. AF2405) were obtained from R&D Systems, Inc. Anti‑NF‑κ B p65 (cat. no. 3033), anti‑pNF‑κ B p65 (cat. no. 3036),

(17) Cell-matrix signals specify bone endothelial cells during developmental osteogenesisNature cell biologyMarch 1, 2017Langen UH, Pitulescu ME, Kim JM, Enriquez-Gasca R, Sivaraj KK, Kusumbe AP, Singh A, Di Russo J, Bixel MG, Zhou B, et al.1:500), rabbit anti-GFP conjugated to AF488 (A21311, Invitrogen, 1:250), goat anti-Integrin beta 1 (AF2405, R&D Systems, 1:50), rabbit anti-Laminin α4 (serum 377, 1:500), rat anti-Laminin α5 (clone 4G6, 1:10),

(18) Exon skipping restores dystrophin expression, but fails to prevent disease progression in later stage dystrophic dko miceGene therapySeptember 1, 2014Wu B, Cloer C, Lu P, Milazi S, Shaban M, Shah SN, Marston-Poe L, Moulton HM, Lu QL.

(19) Methamphetamine-associated cleavage of the synaptic adhesion molecule intercellular adhesion molecule-5Journal of neurochemistryAugust 1, 2011Conant K, Lonskaya I, Szklarczyk A, Krall C, Steiner J, Maguire-Zeiss K, Lim ST.Signaling Technologies (Beverly, MA, USA). Anti-β1 integrin antibodies were obtained from R & D Systems (AF2405) and Millipore Corporation (Bedford, MA, USAAB1952). Texas-Red conjugated phalloidin was purchased from

(20) Loss of beta1 integrin in mouse fibroblasts results in resistance to skin scleroderma in a mouse modelArthritis and rheumatismSeptember 1, 2009Liu S, Kapoor M, Denton CP, Abraham DJ, Leask A.

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Integrin beta 1/CD29 Antibody

Integrin beta 1/CD29 Antibody

Integrin beta 1/CD29 Antibody

Integrin beta 1/CD29 Antibody

Integrin beta 1/CD29 Antibody

Mouse/Rat Integrin ß1/CD29 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (23)

Reviews

Mouse/Rat Integrin ß1/CD29 Antibody from R&D Systems, a Bio-Techne Brand

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