Fig 2: Overexpression of miR-219a-5p suppresses cell viability, migration and invasion and induces apoptosis by targeting SOX5 in NSCLC cells. The mRNA and protein levels of (a and b) SOX5, (c and d) cell viability, (e) colony formation, (f) apoptosis, (g) migration and (h) invasion were detected in A549 and Calu-3 cells transfected with miR-NC, miR-219a-5p, miR-219a-5p and pcDNA or SOX5 by qRT-PCR, western blot, MTT, colony formation, flow cytometry and transwell assays, respectively. *P < 0.05. (a, b, e–h) () miR-NC, () miR-219a-5p, () miR-219a-5p+pcDNA and () miR-219a-5p+SOX5. (c, d) () miR-NC, () miR-219a-5p, () miR-219a-5p+pcDNA and () miR-219a-5p+SOX5.

Fig 3: Knockdown of circCDR1as decreases SOX5 expression by regulating miR-219a-5p in NSCLC cells. The (a) mRNA and (b) protein levels of SOX5 were measured in A549 and Calu-3 cells transfected with si-NC, si-circCDR1as#1, si-circCDR1as#1 and anti-miR-NC or anti-miR-219a-5p by qRT-PCR and western blot. *P < 0.05. () si-NC, () si-circCDR1as#1, () si-circCDR1as#1+anti-miR-NC and () si-circCDR1as#1+anti-miR-219a-5p.