Western blot analysis of SOX5 in pig adipose cells. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with SOX5 polyclonal antibody (Product # PA5-95266) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Western blot analysis of SOX5 in Lane 1: rat liver tissue lysate (50 µg), Lane 2: rat testis tissue lysate (50 µg), Lane 3: rat brain tissue lysate (50 µg), Lane 4: HeLa whole cell lysate (40 µg), Lane 5: A549 whole cell lysate (40 µg). Samples were incubated with SOX5 polyclonal antibody (Product # PA5-95266).