Western blot analysis of METTL3 in Lane 1: human placenta tissue lysates, Lane 2: human HepG2 whole cell lysate, Lane 3: human K562 whole cell lysate, Lane 4: human HeLa whole cell lysate, Lane 5: human Caco-2 whole cell lysate. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with METTL3 polyclonal antibody (Product # PA5-95569) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).