Immunofluorescent analysis of hnRNP K in A549 cells using a hnRNP K monoclonal antibody (Product # MA5-33166) at a dilution of 1:43.5. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L). Cells were counter-stained with DAPI.

Western Blot analysis of hnRNP K using a hnRNP K Monoclonal antibody (Product # MA5-33166) at a concentration of 1.3 µg/mL. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, 293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 60 kDa.