TLR2 Antibody AF2616 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemTLR2 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2616
NCBI Gene AliasesTIL4, CD282
ApplicationsCell-based ELISA,Flow Cytometry,Immunohistochemistry,Immunoprecipitation,Western Blot,Immunohistochemistry-Paraffin,ELISA Capture (Matched Antibody Pair),CyTOF-ready
TargetTLR2
Quantity100 ug (also 25 ug)
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
IsotypeIgG
HostGoat
Conjugate/TagUnconjugated
ReactivityHuman
ImmunogenMouse myeloma cell line NS0-derived recombinant human TLR2, Glu21-Leu590, Accession # O60603
ConcentrationLYOPH
PurityAntigen Affinity-purified
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
Antibody TypePrimary Antibodies
ClonalityPolyclonal
NCBI Full Gene Nametoll like receptor 2

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human TLR2 in ELISAs and Western blots. In sandwich ELISAs, less than 1% cross-reactivity with recombinant mouse TLR2 and less than 0.3% cross-reactivity with recombinant human (rh) TLR3 and rhTLR4/MD-2 is observed.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Distinct Effects of Respiratory Viral Infection Models on miR-149-5p, IL-6 and p63 Expression in BEAS-2B and A549 Epithelial CellsCellsMay 26, 2024Nafeesa Shahdab, Christopher Ward, Philip M. Hansbro, Stephen Cummings, John S. Young, Fatemeh Moheimaniantibody (R&D systems, Bio-techne, AF1487, Abingdon, UK), anti-TLR2 antibody (R&D systems, Bio-techne, AF2616), anti-GAPDH antibody (Abcam, ab9483, Cambridge, UK), donkey anti-goat IgG (HRP, Abcam, ab205723) and

(2) NOS inhibition reverses TLR2-induced chondrocyte dysfunction and attenuates age-related osteoarthritisProceedings of the National Academy of Sciences of the United States of AmericaJuly 18, 2023Shen P, Serve S, Wu P, Liu X, Dai Y, Durán-Hernández N, Nguyen DTM, Fuchs M, Maleitzke T, Reisener MJ, et al.

(3) LYSMD3: A mammalian pattern recognition receptor for chitinCell reportsJuly 20, 2021He X, Howard BA, Liu Y, Neumann AK, Li L, Menon N, Roach T, Kale SD, Samuels DC, Li H, et al.

(4) Identification of TLR2 Signalling Mechanisms Which Contribute to Barrett's and Oesophageal Adenocarcinoma Disease ProgressionCancersApril 25, 2021Flis E, Barber G, Nulty C, Keogh B, McGuirk P, Anand A, O'Sullivan J, Quante M, Creagh EM.

(5) Inhibition of osteoclastogenesis by opsonized Porphyromonas gingivalisFASEB bioAdvancesApril 1, 2019Pandruvada SN, Ebersole JL, Huja SS.quantified by densitometry using ImageJ. The primary antibodies were used at: TLR2 (R and D Systems Cat# AF2616, RRID:http://scicrunch.org/resolver/AB_416645), TLR4 (R and D Systems Cat# AF1478, RRID:http://scicrunch.org/resolver/AB_354816)

(6) Soluble Siglec-14 glycan-recognition protein is generated by alternative splicing and suppresses myeloid inflammatory responsesThe Journal of biological chemistryDecember 21, 2018Huang PJ, Low PY, Wang I, Hsu SD, Angata T.

(7) Placental inflammation by HMGB1 activation of TLR4 at the syncytiumPlacentaDecember 1, 2018Tangerås LH, Silva GB, Stødle GS, Gierman LM, Skei B, Collett K, Beversmark AL, Skråstad RB, Thomsen LCV, Bjørge L, et al.min and peroxidase blocking solution for 5 min (#K4007, Dako). Slides were stained for TLR2 (1:500, #AF2616, R&D Systems), TLR4 (1:175, #MAB14783, R&D Systems), IL-8 (1:200, #60141-2-ig, Proteintech), HMGB1 (1:80,

(8) Toll-like receptor 2 is increased in neurons in Parkinson's disease brain and may contribute to alpha-synuclein pathologyActa neuropathologicaFebruary 1, 2017Dzamko N, Gysbers A, Perera G, Bahar A, Shankar A, Gao J, Fu Y, Halliday GM.

(9) Expression and regulation of toll-like receptors (TLRs) in human intervertebral disc cellsEuropean spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research SocietySeptember 1, 2014Klawitter M, Hakozaki M, Kobayashi H, Krupkova O, Quero L, Ospelt C, Gay S, Hausmann O, Liebscher T, Meier U, et al.(Amersham, Switzerland) as previously described [32]. The membrane was incubated with a TLR2 antibody (AF2616, R&D Systems, United Kingdom) followed by incubation with an appropriate HRP secondary antibody before

(10) Metalloproteinase-dependent TLR2 ectodomain shedding is involved in soluble toll-like receptor 2 (sTLR2) productionPloS oneJanuary 1, 2014Langjahr P, Díaz-Jiménez D, De la Fuente M, Rubio E, Golenbock D, Bronfman FC, Quera R, González MJ, Hermoso MA.

(11) ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytesThe Journal of biological chemistryAugust 3, 2012Wilhelmsen K, Mesa KR, Lucero J, Xu F, Hellman J.

(12) Activation of endothelial TLR2 by bacterial lipoprotein upregulates proteins specific for the neutrophil responseInnate immunityAugust 1, 2012Wilhelmsen K, Mesa KR, Prakash A, Xu F, Hellman J.temperature (RT) and then cut in half. The top half was incubated with anti-human TLR2 (0.5 µg/ml; AF2616; R&D Systems, Minneapolis, MN) and the bottom half with anti-actin rabbit polyclonal antibody (1:10000;

(13) Maternal high-fat diet disturbs uteroplacental hemodynamics and increases the frequency of stillbirth in a nonhuman primate model of excess nutritionEndocrinologyJune 1, 2011Frias AE, Morgan TK, Evans AE, Rasanen J, Oh KY, Thornburg KL, Grove KL.

(14) Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivoJournal of immunology (Baltimore, Md. : 1950)January 15, 2011Shin HS, Xu F, Bagchi A, Herrup E, Prakash A, Valentine C, Kulkarni H, Wilhelmsen K, Warren S, Hellman J.monoclonal IgG (1:1000, #05-881, Upstate, Waltham, MA), anti-human TLR2 goat polyclonal IgG (0.5 μg/ml, AF2616, R&D Systems) or anti-actin goat polyclonal antibody (Sigma-Aldrich). After developing the immunoblots,

(15) Cell Type-specific Expression and Function of Toll-like Receptors 2 and 4 in Human Placenta: Implications in Fetal InfectionPlacentaOctober 1, 2007Y. Ma, G. Krikun, V.M. Abrahams, G. Mor, S. Gullerplacental villi. Immunolocalization of TLRs was carried out using goat anti-human antibodies to TLR-2 (AF2616, R&D Systems, Minneapolis, MN, 1:500 dilution) and TLR-4 (AF1478, R&D Systems, 1:1000). For negative

(16) PAUF Induces Migration of Human Pancreatic Cancer Cells Exclusively via the TLR4/MyD88/NF-κB Signaling PathwayInternational journal of molecular sciencesJanuary 1, 0001"Youn SE, Jiang F, Won HY, Hong DE, Kang TH, Park YY, Koh SS."Waltham, MA, USA) was used as a control antibody for the anti-PAUF antibody. Antibodies against TLR2 (cat# AF2616), TLR4 (cat# AF1478), and Goat IgG-phycoerythrin (IgG-PE, cat# F0107) were purchased from R&D Systems, Inc

(17) Evidence of a role for interleukin-6 in anoikis resistance in oral squamous cell carcinoma"Medical oncology (Northwood, London, England)"January 1, 0001"Karavyraki M, Porter RK."

(18) Platelets mediate inflammatory monocyte activation by SARS-CoV-2 spike proteinThe Journal of clinical investigationJanuary 1, 0001"Li T, Yang Y, Li Y, Wang Z, Ma F, Luo R, Xu X, Zhou G, Wang J, Niu J, et al."anti-ACE-2 (R&D Systems, AF933), anti-GRP78 (Novus Biological, NB100-56411), anti-TLR2 (R&D Systems, AF2616), anti-CD42b (Abcam, EPR19204), anti–S-RBD (Sino Biological, 40592-R001), anti–phospho-(Ser) PKC substrates

(19) CCN1 is an opsonin for bacterial clearance and a direct activator of Toll-like receptor signalingNature communicationsJanuary 1, 0001"Jun JI, Lau LF."well, R&D systems) were incubated with coated CCN1 proteins and detected using polyclonal anti-hTLR2 (AF2616), anti-hTLR4 (AF1478), or monoclonal anti-hCD14 antibodies (MAB3832, R&D systems; 1:100 dilution each,)

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Human TLR2 Antibody

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TLR2 Antibody

TLR2 Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (19)

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TLR2 Antibody from R&D Systems, a Bio-Techne Brand

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