Western blot was performed using Anti-Galectin 9 Polyclonal Antibody (Product # PA5-85496) and a ~36 kDa band corresponding to 36 kDa beta-galactoside-binding lectin was observed across cell lines tested. Whole cell extracts (15 µg lysate) of HUVEC (Lane 1), THP-1 (Lane 2), THP-1 treated with Rec. human TGF beta 1 (2 ng/mL for 24 hrs) (Lane 3), U-937 (Lane 4), MOLT-4 (Lane 5), PANC-1 (Lane 6), SH-SY5Y (Lane 7), Daudi (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). Additional non specific band (*) was observed around 110 kDa.