Immunofluorescence staining of HVEM in Raji cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with TNFRSF14 Recombinant Rabbit Monoclonal Antibody (28) (Product # MA5-30950, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and Cell membrane.