Immunocytochemistry-Immunofluorescence analysis of ZNF9 was performed in U-2 OS cells using ZNF9 Polyclonal Antibody (Product # PA5-90229) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of ZNF9 in various lysates. Samples were incubated with ZNF9 Polyclonal antibody (Product # PA5-90229) using a dilution of 1:800, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.