Immunocytochemistry-Immunofluorescence analysis of GSK3 alpha was performed in NIH/3T3 cells using GSK3 alpha Polyclonal Antibody (Product # PA5-87470) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of GSK3 alpha in extracts of various cell lines. Samples were incubated with GSK3 alpha Polyclonal antibody (Product # PA5-87470) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.